1887

Abstract

is an opportunistic human pathogen which poses a major threat to patients with cystic fibrosis (CF). Excessive amounts of mucus present in the lungs of CF patients promotes the colonization of . The gene, encoding a putative glycosyltransferase, has been shown to be highly inducible by respiratory mucus derived from CF patients. In this study, it is further demonstrated by population transcript analysis that the gene is highly expressed in the CF lung environment. Deletion analysis of the promoter identified a -box-like sequence commonly found in promoters that are responsive to quorum sensing regulation. Further analysis of expression in quorum-sensing-defective strains, as well as its expression in response to autoinducer molecules, demonstrated that is regulated by the RhlI/RhlR quorum sensing regulatory system. Functionally, as the MigA sequence homology data suggested, the gene indeed affects the structure of LPS in . Increased expression of the gene results in a loss of core-plus-one LPS, while having no obvious effect on the long-chain O-antigen-bearing LPS. Although the exact biological role of the core-plus-one LPS is not clear, these experimental results suggest that up-regulation in the CF lung environment is part of the adaptive response which confers on a survival advantage.

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2000-10-01
2019-10-18
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