1887

Abstract

Several different species of produce -acylhomoserine lactones (AHLs), quorum-sensing signal molecules which are involved in the cell-density-dependent control of secondary metabolite and virulence gene expression. When F113 was cross-streaked against AHL biosensors capable of sensitively detecting either short (C–C) or long (C–C) acyl chain AHLs, no activity was detectable. However, by extracting cell-free stationary-phase culture supernatants with dichloromethane followed by reverse-phase HPLC, three distinct fractions were obtained capable of activating the AHL biosensors. Three AHLs were subsequently characterized using high-resolution MS and chemical synthesis. These were (i) -(3-hydroxy-7--tetradecenoyl)homoserine lactone (3OH,C-HSL), a molecule previously known as the bacteriocin as a consequence of its growth inhibitory properties, (ii) -decanoylhomoserine lactone (C-HSL) and (iii) -hexanoylhomoserine lactone (C-HSL). A gene () capable of directing synthesis of all three AHLs in was cloned and sequenced. transcription/translation of yielded a protein of approximately 33 kDa capable of directing the synthesis of 3OH,C-HSL, C-HSL and C-HSL in . HdtS does not belong to either of the known AHL synthase families (LuxI or LuxM) and is related to the lysophosphatidic acid acyltransferase family. HdtS may therefore constitute a member of a third protein family capable of AHL biosynthesis.

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2000-10-01
2020-10-22
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