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Volume 146, Issue 10

Regulatory interactions between the Hrp type III protein secretion system and coronatine biosynthesis in pv. DC3000 Free

Abstract

In , the co-ordinated regulation of different systems required for pathogenicity and virulence seems logical but has not been established. This question was addressed in the present study by analysing production of the phytotoxin coronatine (COR) in defined / mutants of pv. DC3000. COR was produced by mutants of DC3000 defective in , which encodes an outer-membrane protein required for type III-mediated secretion. When inoculated in plants, mutants produced chlorotic regions indicative of COR production, but lacked the necrotic lesions produced by the wild-type DC3000. Furthermore, a DC3000 mutant containing a polar mutation in , which inactivates , and , produced significantly higher amounts of COR than the wild-type strain . This mutant was able to produce COR earlier and at lower cell densities than the wild-type. The results indicate that the / secretion system is not required for COR production, but mutations in this system may have regulatory effects on the production of virulence factors such as COR.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): CFA, coronafacic acid , CMA, coronamic acid , COR, coronatine , GUS, glucuronidase , HR, hypersensitive response , HSS medium, Hoitink–Sinden medium amended with sucrose , MG medium, mannitol-glutamate medium , phytotoxin , type III secretion system and virulence
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2000-10-01
2024-04-20
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Regulatory interactions between the Hrp type III protein secretion system and coronatine biosynthesis in Pseudomonas syringae pv. tomato DC3000
Microbiology 146, 2447 (2000); https://doi.org/10.1099/00221287-146-10-2447
/content/journal/micro/10.1099/00221287-146-10-2447
/content/journal/micro/10.1099/00221287-146-10-2447
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