1887

Abstract

After a shift of from aerobic to anaerobic growth conditions, nitrate ammonification and various fermentative processes replace oxygen-dependent respiration. Cell-free extracts prepared from wild-type and from mutants of the regulatory loci and grown under aerobic and various anaerobic conditions were compared by two-dimensional gel electrophoresis. Proteins involved in the adaptation process were identified by their N-terminal sequence. Induction of cytoplasmic lactate dehydrogenase (LctE) synthesis under anaerobic fermentative conditions was dependent on and . Anaerobic nitrate repression of LctE formation required -mediated expression of , encoding respiratory nitrate reductase. Anaerobic induction of the flavohaemoglobin Hmp required and nitrite. The general anaerobic induction of , encoding a protein of unknown function, was modulated by and . The gene shares its upstream region with the gene, encoding the fermentative enzyme acetyl-CoA:orthophosphate acetyltransferase. Anaerobic repression of the synthesis of a potential membrane-associated NADH dehydrogenase (YjlD, Ndh), and anaerobic induction of fructose-1,6-bisphosphate aldolase (FbaA) and dehydrolipoamide dehydrogenase (PhdD, Lpd) formation, did not require or participation. Synthesis of glycerol kinase (GlpK) was decreased under anaerobic conditions. Finally, the effect of anaerobic stress induced by the immediate shift from aerobic to strictly anaerobic conditions was analysed. The induction of various systems for the utilization of alternative carbon sources such as inositol (IolA, IolG, IolH, IolI), melibiose (MelA) and 6-phospho-α-glucosides (GlvA) indicated a catabolite-response-like stress reaction.

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2000-01-01
2020-01-21
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