1887

Abstract

Adhesion of to saliva-coated surfaces is an important early step in the colonization of the oral cavity. cells also adhere to several species of oral streptococci including , and in what are believed to be multi-modal interactions. It is now demonstrated that incubation of streptococcal cells of these species with human parotid saliva further promotes the adhesion of cells by up to 23-fold. Various species of streptococci were shown to adsorb different protein components of parotid saliva to their cell surfaces. The basic proline-rich proteins (bPRPs), to which cells bind on nitrocellulose blot overlay, were strongly adsorbed to the surface of cells but not to cells. Parotid saliva that was pre-adsorbed with cells and then applied to hydroxylapatite beads was <50% effective at supporting adhesion of compared with control (non-adsorbed) saliva, demonstrating that bPRPs are major pellicle receptors. cells did not adsorb bPRPs from fluid-phase parotid saliva. Following size-exclusion chromatography of parotid saliva samples, pooled fractions enriched in bPRPs promoted maximal adhesion of to cells. The results demonstrate that cells recognize only surface-bound forms of bPRPs and suggest that these proteins adsorbed to enamel or to streptococcal surfaces promote adhesion and oral colonization.

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2000-01-01
2020-01-18
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References

  1. Arendorf T. M., Walker D. M.. 1979; Oral candidal populations in health and disease. Br Dent J147:267–272[CrossRef]
    [Google Scholar]
  2. Arendorf T. M., Walker D. M.. 1980; The prevalence and intra-oral distribution of Candida albicans in man. Arch Oral Biol25:1–10[CrossRef]
    [Google Scholar]
  3. Branting C., Sund M. L., Linder L. E.. 1989; The influence of Streptococcus mutans on adhesion of Candida albicans to acrylic surfaces in vitro. Arch Oral Biol34:347–353[CrossRef]
    [Google Scholar]
  4. Cannon R. D., Chaffin W. L.. 1999; Oral colonization by Candida albicans. Crit Rev Oral Biol Med10:359–383[CrossRef]
    [Google Scholar]
  5. Cannon R. D., Holmes A. R., Mason A. B., Monk B. C.. 1995a; Oral Candida: clearance, colonization, or candidiasis?. J Dent Res74:1152–1161[CrossRef]
    [Google Scholar]
  6. Cannon R. D., Nand A. K., Jenkinson H. F.. 1995b; Adherence of Candida albicans to human salivary components adsorbed to hydroxylapatite. Microbiology141:213–219[CrossRef]
    [Google Scholar]
  7. Douglas C. W., Russell R. R.. 1984; The adsorption of human salivary components to strains of the bacterium Streptococcus mutans. Arch Oral Biol29:751–757[CrossRef]
    [Google Scholar]
  8. Douglas C. W. I., Pease A. A., Whiley R. A. 1990; Amylase-binding as a discriminator among oral streptococci. FEMS Microbiol Lett66:193–198[CrossRef]
    [Google Scholar]
  9. Edgerton M., Scannapieco F. A., Reddy M. S., Levine M. J.. 1993; Human submandibular-sublingual saliva promotes adhesion of Candida albicans to polymethylmethacrylate. Infect Immun61:2644–2652
    [Google Scholar]
  10. Gibbons R. J., Hay D. I.. 1988; Human salivary acidic proline-rich proteins and statherin promote the attachment of Actinomyces naeslundii LY7 to apatitic surfaces. Infect Immun56:439–445
    [Google Scholar]
  11. Gibbons R. J., Hay D. I., Childs W. C. 3rd, Davis G.. 1990; Role of cryptic receptors (cryptitopes) in bacterial adhesion to oral surfaces. Arch Oral Biol35:107S–114S[CrossRef]
    [Google Scholar]
  12. Gibbons R. J., Hay D. I., Schlesinger D. H.. 1991; Delineation of a segment of adsorbed salivary acidic proline-rich proteins which promotes adhesion of Streptococcus gordonii to apatitic surfaces. Infect Immun59:2948–2954
    [Google Scholar]
  13. Grimaudo N. J., Nesbitt W. E., Clark W. B.. 1996; Coaggregation of Candida albicans with oral Actinomyces species. Oral Microbiol Immunol11:59–61[CrossRef]
    [Google Scholar]
  14. Hodson J. J., Craig G. T.. 1972; The incidence of Candida albicans in the plaques of teeth of children. Dent Pract Dent Rec22:296–301
    [Google Scholar]
  15. Holmes A. R., Shepherd M. G.. 1988; Nutritional factors determine germ tube formation in Candida albicans. J Med Vet Mycol26:127–131[CrossRef]
    [Google Scholar]
  16. Holmes A. R., Cannon R. D., Jenkinson H. F.. 1995a; Interactions of Candida albicans with bacteria and salivary molecules in oral biofilms. J Ind Microbiol15:208–213[CrossRef]
    [Google Scholar]
  17. Holmes A. R., Gopal P. K., Jenkinson H. F.. 1995b; Adherence of Candida albicans to a cell surface polysaccharide receptor on Streptococcus gordonii. Infect Immun63:1827–1834
    [Google Scholar]
  18. Holmes A. R., McNab R., Jenkinson H. F.. 1996; Candida albicans binding to the oral bacterium Streptococcus gordonii involves multiple adhesin-receptor interactions. Infect Immun64:4680–4685
    [Google Scholar]
  19. Iontcheva I., Oppenheim F. G., Troxler R. F.. 1997; Human salivary mucin MG1 selectively forms heterotypic complexes with amylase, proline-rich proteins, statherin, and histatins. J Dent Res76:734–743[CrossRef]
    [Google Scholar]
  20. Jenkinson H. F., Demuth D. R.. 1997; Structure, function, and immunogenicity of streptococcal antigen I/II polypeptides. Mol Microbiol23:183–190[CrossRef]
    [Google Scholar]
  21. Jenkinson H. F., Lamont R. J.. 1997; Streptococcal adhesion and colonization. Crit Rev Oral Biol Med8:175–200[CrossRef]
    [Google Scholar]
  22. Jenkinson H. F., Lala H. C., Shepherd M. G.. 1990; Coaggregation of Streptococcus sanguis and other streptococci with Candida albicans. Infect Immun58:1429–1436
    [Google Scholar]
  23. Kimura L. H., Pearsall N. N.. 1978; Adherence of Candida albicans to human buccal epithelial cells. Infect Immun21:64–68
    [Google Scholar]
  24. Kolenbrander P. E., London J.. 1993; Adhere today, here tomorrow: oral bacterial adherence. J Bacteriol175:3247–3252
    [Google Scholar]
  25. Laemmli U. K.. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature227:680–685[CrossRef]
    [Google Scholar]
  26. Lamont R. J., Rosan B.. 1990; Adherence of mutans streptococci to other oral bacteria. Infect Immun58:1738–1743
    [Google Scholar]
  27. Lynch D. P., Tenn M.. 1994; Oral candidiasis. History, classification, and clinical presentation. Oral Surg Oral Med Oral Pathol78:189–193[CrossRef]
    [Google Scholar]
  28. McNab R., Jenkinson H. F., Loach D. M., Tannock G. W.. 1994; Cell-surface-associated polypeptides CshA and CshB of high molecular mass are colonization determinants in the oral bacterium Streptococcus gordonii. Mol Microbiol14:743–754[CrossRef]
    [Google Scholar]
  29. Moreno E. C., Kresak M., Hay D. I.. 1984; Adsorption of molecules of biological interest onto hydroxyapatite. Calcif Tissue Int36:48–59[CrossRef]
    [Google Scholar]
  30. Newman F., Beeley J. A., MacFarlane T. W., Gailbraith J., Buchanan L.. 1993; Salivary protein interactions with oral bacteria: an electrophoretic study. Electrophoresis14:1322–1327[CrossRef]
    [Google Scholar]
  31. Newman F., Beeley J. A., MacFarlane T. W.. 1996; Adherence of oral micro-organisms to human parotid salivary proteins. Electrophoresis17:266–270[CrossRef]
    [Google Scholar]
  32. Nikawa H., Hamada T.. 1990; Binding of salivary or serum proteins to Candida albicans in vitro. Arch Oral Biol35:571–573[CrossRef]
    [Google Scholar]
  33. Nikawa H., Hayashi S., Nikawa Y., Hamada T., Samaranayake L. P.. 1993; Interactions between denture lining material, protein pellicles and Candida albicans. Arch Oral Biol38:631–634[CrossRef]
    [Google Scholar]
  34. Odds F. C.. 1988; Candida and Candidosis: a Review and Bibliography London: Baillière Tindall;
    [Google Scholar]
  35. O’Sullivan J. M., Cannon R. D., Sullivan P. A., Jenkinson H. F.. 1997; Identification of salivary basic proline-rich proteins as receptors for Candida albicans adhesion. Microbiology143:341–348[CrossRef]
    [Google Scholar]
  36. Pakula R., Walczak W.. 1963; On the nature of competence of transformable streptococci. J Gen Microbiol31:125–133[CrossRef]
    [Google Scholar]
  37. Rams T. E., Slots J.. 1991; Candida biotypes in human adult periodontitis. Oral Microbiol Immunol6:191–192[CrossRef]
    [Google Scholar]
  38. Rogers J. D., Haase E. M., Brown A. E., Douglas C. W., Gwynn J. P., Scannapieco F. A.. 1998; Identification and analysis of a gene (abpA) encoding a major amylase-binding protein in Streptococcus gordonii. Microbiology144:1223–1233[CrossRef]
    [Google Scholar]
  39. Rudney J. D., Ji Z., Larson C. J., Liljemark W. F., Hickey K. L.. 1995; Saliva protein binding to layers of oral streptococci in vitro and in vivo. J Dent Res74:1280–1288[CrossRef]
    [Google Scholar]
  40. Samaranayake L. P.. 1992; Oral mycoses in HIV infection. Oral Surg Oral Med Oral Pathol73:171–180[CrossRef]
    [Google Scholar]
  41. Samaranayake L. P., MacFarlane T. W.. 1982; Factors affecting the in vitro adherence of the fungal oral pathogen Candida albicans to epithelial cells of human origin. Arch Oral Biol27:869–873[CrossRef]
    [Google Scholar]
  42. Scannapieco F. A., Bergey E. J., Reddy M. S., Levine M. J.. 1989; Characterization of salivary α-amylase binding to Streptococcus sanguis. Infect Immun57:2853–2863
    [Google Scholar]
  43. Shannon I. L., Prigmore J. R., Chauncey H. H.. 1962; Modified Carlson–Crittenden device for the collection of parotid fluid. J Dent Res41:778–783[CrossRef]
    [Google Scholar]
  44. Slots J., Rams T. E., Listgarten M. A.. 1988; Yeasts, enteric rods and pseudomonads in the subgingival flora of severe adult periodontitis. Oral Microbiol Immunol3:47–52[CrossRef]
    [Google Scholar]
  45. Stinson M. W., Haraszthy G. G., Zhang X. L., Levine M. J.. 1992; Inhibition of Porphyromonas gingivalis adhesion to Streptococcus gordonii by human submandibular-sublingual saliva. Infect Immun60:2598–2604
    [Google Scholar]
  46. Towbin H., Staehelin T., Gordon J.. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA76:4350–4354[CrossRef]
    [Google Scholar]
  47. Vasilas A., Molina L., Hoffman M., Haidaris C. G.. 1992; The influence of morphological variation on Candida albicans adhesion to denture acrylic in vitro. Arch Oral Biol37:613–622[CrossRef]
    [Google Scholar]
  48. Verran J., Motteram K. L.. 1987; The effect of adherent oral streptococci on the subsequent adherence of Candida albicans to acrylic in vitro. J Dent15:73–76[CrossRef]
    [Google Scholar]
  49. Whittaker C. J., Klier C. M., Kolenbrander P. E.. 1996; Mechanisms of adhesion by oral bacteria. Annu Rev Microbiol50:513–552[CrossRef]
    [Google Scholar]
  50. Wright P. S., Clark P., Hardie J. M.. 1985; The prevalence and significance of yeasts in persons wearing complete dentures with soft-lining materials. J Dent Res64:122–125[CrossRef]
    [Google Scholar]
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