1887

Abstract

Purified glutamate synthase (GOGAT) from was characterized as a high-molecular-mass polypeptide, a distinction shared with previously described GOGATs from other eukaryotic micro-organisms. Using degenerate deoxyoligonucleotides, designed from conserved regions of the alfalfa, maize and GOGAT genes, a 300 bp PCR fragment from the GOGAT gene was obtained. This fragment was used to construct null GOGAT mutants of by gene replacement. These mutants showed no growth defect phenotype and were able to grow on ammonium as sole nitrogen source. Double mutants obtained from a cross between a previously described mutant and the null GOGAT strain were full glutamate auxotrophs. These results indicate that glutamate biosynthesis in . is afforded through the combined action of and products.

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2000-01-01
2019-10-13
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