1887

Abstract

The roles of cyclic formaldehyde oxidation via 6-phosphogluconate dehydrogenase and linear oxidation via the tetrahydromethanopterin (HMPT)-linked pathway were assessed in an obligate methylotroph, KT, by cloning, sequencing and mutating two chromosomal regions containing genes encoding enzymes specifically involved in these pathways:6-phosphogluconate dehydrogenase, glucose-6-phosphate dehydrogenase and methenyl HMPT cyclohydrolase (, and ). No null mutants were obtained in or , implying that the cyclic oxidation of formaldehyde is required for C metabolism in this obligate methylotroph, probably as the main energy-generating pathway. In contrast, null mutants were generated in , indicating that the HMPT-linked pathway is dispensable. These mutants showed enhanced sensitivity to formaldehyde, suggesting that this pathway plays a secondary physiological role in this methylotroph. This function is in contrast to AM1, in which the HMPT-linked pathway is essential.

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2000-01-01
2019-11-20
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