1887

Abstract

The region of the genome was sequenced, revealing an incomplete set of five genes encoding all-but-one of the enzymes involved in the biosynthesis of mycarose. The latter is a 6-deoxyhexose sugar required during production of the macrolide antibiotic, tylosin. The missing mycarose-biosynthetic gene, , was found about 50 kb distant from its functional partners, on the other side of the (polyketide synthase) gene complex. Mutational analysis, involving targeted gene transplacement, was employed to confirm the functions of specific genes, including . Particularly interesting was the similarity between the tylosin-biosynthetic mycarosyltransferase enzyme, TylCV, and proteins of the macrolide glycosyltransferase (MGT) family that inactivate macrolides via glycosylation of attached sugar residues and are involved in resistance and/or antibiotic efflux. The arrangement of genes within the ‘mycarose cluster’ would allow their expression as two short operons with divergent, and perhaps co-regulated, promoters. Whether displacement of relative to the other genes provides additional regulatory opportunities remains to be established.

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2000-01-01
2020-01-27
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