1887

Abstract

The DNA gyrases from , and bv. , which are species naturally resistant, moderately susceptible and susceptible to fluoroquinolones, respectively, were purified by affinity chromatography on novobiocin-Sepharose columns. The DNA gyrase inhibiting activities (IC values) of classical quinolones and fluoroquinolones were determined from the purified enzymes and were compared to the corresponding antibacterial activities (MICs). Regarding bv. , which is nearly as susceptible as , the corresponding MIC and IC values of quinolones were significantly lower than those found for and (e.g. for ofloxacin, MICs of 025 versus 32 and 1 μg ml, and IC values of 1 versus 8 and 6 μg ml, respectively). Such a result could be related to the presence of Ser-83 in the quinolone-resistance-determining region of the gyrase A subunit of bv. , as found in wild-type , instead of Ala-83 in and , as found in fluoroquinolone-resistant mutants. The IC values of quinolones against the and DNA gyrases were similar, while the corresponding MICs were 32-fold higher for when compared to , suggesting that an additional mechanism, such as a low cell wall permeability or a drug efflux, could contribute to the low antibacterial potency of quinolones against .

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1999-09-01
2019-10-19
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