1887

Abstract

To survive and multiply in different environments, has to co-ordinately regulate the expression of genes involved in adaptive responses. In many pathogens, adaptive responses, including pathogenic responses, are regulated by two-component regulator (TCR) systems. It is likely that members of a TCR family play a role in the regulation of processes involved in intestinal colonization, and therefore pathogenesis, in . We have identified and characterized a TCR system of : this system is a homologue of PhoBR. The presence of a putative Pho box suggests that the operon is regulated by inorganic phosphate levels. The and genes are organized the same way as in . Mutation of the gene affected the expression of the putative Pho regulon, including PhoA, but did not affect the production of cholera toxin. mutants are less able to colonize rabbit intestine than wild-type . The addition of inorganic phosphate at a high concentration to the inoculum only partially restored the ability of the mutants to colonize the intestine, suggesting that the Pho regulon may not be regulated by inorganic phosphate levels alone.

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1999-09-01
2019-10-15
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