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Volume 145, Issue 9

Evidence for a general-purpose genotype in , highly prevalent in multiple geographical regions, patient types and types of infection Free

Abstract

Epidemiological studies, using the probe Ca3, have shown that in a given patient population a single cluster of genetically related isolates usually predominates. The authors have investigated whether these local clusters are part of a single group, geographically widespread and highly prevalent as an aetiological agent of various types of candidiasis. An unrooted neighbour-joining tree of 266 infection-causing . isolates (each from a different individual) from 12 geographical regions in 6 countries was created, based on genetic distances generated by Ca3 fingerprinting. Thirty-seven per cent of all isolates formed a single genetically homogeneous cluster (cluster A). The remainder of isolates were genetically diverse. Using the maximum branch length within cluster A as a cut-off, they could be divided into 37 groups, whose prevalence ranged between 03% and 9%. Strains from cluster A were highly prevalent in all but one geographical region, with a mean prevalence across all regions of 41%. When isolates were separated into groups based on patient characteristics or type of infection, strains from cluster A had a prevalence exceeding 27% in each group, and their mean prevalence was 43% across all patient characteristics. These data provide evidence that cluster A constitutes a general-purpose genotype, which is geographically widespread and acts as a predominant aetiological agent of all forms of candidiasis in all categories of patients surveyed.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): evolution , Ca3 fingerprinting , Candida albicans , general-purpose genotype , pathogenesis and UPGMA, unweighted pair group method using arithmetic averages
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1999-09-01
2024-04-18
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References

  1. Caugant D. A., Sandven P. 1993; Epidemiological analysis of Candida albicans strains by multilocus enzyme electrophoresis. . J Clin Microbiol 31:215–220
     [Google Scholar]
  2. Dawkins R. , Krebs J. R. 1979; Arms races between and within species. In The Evolution of Adaptation by Natural Selection pp 55–77Edited by Smith J. M., Holliday R. Cambridge: Cambridge University Press;
     [Google Scholar]
  3. Fox J. A., Dybdahl M. F., Jokela J. , Lively C. M. 1996; Genetic structure of coexisting sexual and clonal subpopulations in a freshwater snail ( Potamopyrgus antipodarum). Evolution 50:1541–1548 [CrossRef]
     [Google Scholar]
  4. Giblin L., Edelmann A., Maltzahn N. B. v., Cleland S. B., Schmid J. 1998; A DNA fragment associated with the success of Candida albicans strains as pathogens. Abstracts, Sixth International Mycological Congress, Jerusalem, Israel, p 12
     [Google Scholar]
  5. Gräser Y., Volovsek M., Arrington J., Schönian G., Presber W., Mitchell T. G., Vilgalys R. 1996; Molecular markers reveal that population structure of the human pathogen Candida albicans exhibits both clonality and recombination. Proc Natl Acad Sci USA 93:12473–12477 [CrossRef]
     [Google Scholar]
  6. Hellstein J. , Vawter-Hugart, H. , Fotos, P. , Schmid, J. , Soll D. R. 1993; Genetic similarity and phenotypic diversity of commensal and pathogenic strains of Candida albicans isolated from the oral cavity. J Clin Microbiol 31:3190–3199
     [Google Scholar]
  7. Hermanutz L. A., Weaver S. E. 1996; Agroecotypes or phenotypic plasticity? Comparison of agrestal and ruderal populations of the weed Solanum ptycanthum. Oecologia 105:271–280 [CrossRef]
     [Google Scholar]
  8. Hunter P. R. 1991; The sub-specific numerical analysis of Candida albicans. J Med Vet Mycol 29:105–115 [CrossRef]
     [Google Scholar]
  9. Jacobsen R. , Forbes V. E. 1997; Clonal variation in life-history traits and feeding rates in the gastropod, Potamopyrgus antipodarum: performance across a salinity gradient. . Funct Ecol 11:260–267 [CrossRef]
     [Google Scholar]
  10. Lanyon S. M. 1985; Detecting internal inconsistencies in distance data. Syst Zool 34:397–403 [CrossRef]
     [Google Scholar]
  11. Lapointe F.-J. , Kirsch, J. A. W. , Bleiweiss R. 1994; Jackknifing of weighted trees: validation of phylogenies reconstructed from distance matrices. . Mol Phylogenet Evol 3:256–267 [CrossRef]
     [Google Scholar]
  12. Odds F. C. 1958 Candida and Candidosis, 2nd edn. London: Baillière Tindall;
     [Google Scholar]
  13. Ørskov F., Ørskov I. 1983; Summary of a workshop on the clone concept in the epidemiology, taxonomy, and evolution of the enterobacteriaceae and other bacteria. J Infect Dis 148:346–357 [CrossRef]
     [Google Scholar]
  14. Parker E. D. J. , Selander R. K. 1975; The organization of genetic diversity in the parthenogenetic lizard Cnemidophorus tesselatus. Genetics 84:791–805
     [Google Scholar]
  15. Parker E. D. J., Selander R. K., Hudson R. O., Lester L. J. 1977; Genetic diversity in colonizing parthenogenetic cockroaches. Evolution 31:836–842 [CrossRef]
     [Google Scholar]
  16. Pfaller M. A., Lockhart S. R., Pujol C., Swails-Wenger J. A., Messer S. A., Edmont M. B., Jones R. N., Wenzel R. P., Soll D. R. 1998; Hospital specificity, region specificity, and fluconazole resistance of Candida albicans bloodstream isolates. J Clin Microbiol 36:1518–1529
     [Google Scholar]
  17. Pujol C., Reynes J., Renaud F., Raymond M., Tibayrenc M., Ayala F. J., Janbon F., Mallié M., Bastide J.-M. 1993; The yeast Candida albicans has a clonal mode of reproduction in a population of infected human immunodeficiency virus-positive patients. Proc Natl Acad Sci USA 90:9456–9459 [CrossRef]
     [Google Scholar]
  18. Pujol C. , Joly, S. , Lockhart, S. R. , Noel, S. , Tibayrenc, M. , Soll D. R. 1997; Parity among the randomly amplified polymorphic DNA method, multilocus enzyme electrophoresis and Southern blot hybridization with the moderately repetitive DNA probe Ca3 for fingerprinting Candida albicans. . J Clin Microbiol 35:2348–2358
     [Google Scholar]
  19. Schmid J. 1993; Candidiasis: conclusions from DNA fingerprinting. Clin Adv Treatment Fungal Infect 4:12–16
     [Google Scholar]
  20. Schmid J. , Voss, E. , Soll D. R. 1990; Computer-assisted methods for assessing strain relatedness in Candida albicans by fingerprinting with the moderately repetitive sequence Ca3. . J Clin Microbiol 28:1236–1243
     [Google Scholar]
  21. Schmid J. , Odds, F. C. , Wiselka, M. J. , Nicholson, K. G. , Soll D. R. 1992; Genetic similarity and maintenance of Candida albicans strains from a group of AIDS patients, demonstrated by DNA fingerprinting. J Clin Microbiol 30:935–941
     [Google Scholar]
  22. Schmid J. , Rotman, M. , Reed, B. , Pierson, C. L. , Soll D. R. 1993; Genetic similarity of Candida albicans strains from vaginitis patients and their partners. J Clin Microbiol 31:39–46
     [Google Scholar]
  23. Schmid J. , Hunter, P. R. , White, G. C. , Nand, A. K. , Cannon R. D. 1995a; Physiological traits associated with success of Candida albicans strains as commensal colonisers and pathogens. J Clin Microbiol 33:2920–2926
     [Google Scholar]
  24. Schmid J. , Tay, Y. P. , Wan, L. , Carr, M. , Parr, D. , McKinney W. 1995b; Evidence for nosocomial transmission of Candida albicans obtained by Ca3 fingerprinting. J Clin Microbiol 33:1223–1230
     [Google Scholar]
  25. Semlitsch R. D., Hotz H. , Guex G. D. 1997; Competition among tadpoles of coexisting hemiclones of hybridogenetic Rana esculenta : support for the frozen niche variation model. Evolution 51:1249–1261 [CrossRef]
     [Google Scholar]
  26. Swofford D. L., Olsen G. J., Waddell P. J., Hillis D. M. 1996; Phylogenetic inference. In Molecular Systematics pp 407–514Edited by Hillis D. M., Moritz C., Mable B. K. Sunderland, MA: Sinauer Associates;
     [Google Scholar]
  27. Tibayrenc M. 1997; Are Candida albicans natural populations subdivided?. Trends Microbiol 5:253–257 [CrossRef]
     [Google Scholar]
  28. Tibayrenc M. , Ayala F. J. 1988; Isozyme variability in Trypanosoma cruzi, the agent of Chagas’ disease: genetical, taxonomical and epidemiological significance. . Evolution 42:277–292 [CrossRef]
     [Google Scholar]
  29. Whittam T. S., Ochman H. , Selander R. K. 1983; Multilocus genetic structure in natural populations of Escherichia coli. . Proc Natl Acad Sci 80:1751–1755 [CrossRef]
     [Google Scholar]
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Evidence for a general-purpose genotype in Candida albicans , highly prevalent in multiple geographical regions, patient types and types of infection
Microbiology 145, 2405 (1999); https://doi.org/10.1099/00221287-145-9-2405
/content/journal/micro/10.1099/00221287-145-9-2405
/content/journal/micro/10.1099/00221287-145-9-2405
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