1887

Abstract

Epidemiological studies, using the probe Ca3, have shown that in a given patient population a single cluster of genetically related isolates usually predominates. The authors have investigated whether these local clusters are part of a single group, geographically widespread and highly prevalent as an aetiological agent of various types of candidiasis. An unrooted neighbour-joining tree of 266 infection-causing . isolates (each from a different individual) from 12 geographical regions in 6 countries was created, based on genetic distances generated by Ca3 fingerprinting. Thirty-seven per cent of all isolates formed a single genetically homogeneous cluster (cluster A). The remainder of isolates were genetically diverse. Using the maximum branch length within cluster A as a cut-off, they could be divided into 37 groups, whose prevalence ranged between 03% and 9%. Strains from cluster A were highly prevalent in all but one geographical region, with a mean prevalence across all regions of 41%. When isolates were separated into groups based on patient characteristics or type of infection, strains from cluster A had a prevalence exceeding 27% in each group, and their mean prevalence was 43% across all patient characteristics. These data provide evidence that cluster A constitutes a general-purpose genotype, which is geographically widespread and acts as a predominant aetiological agent of all forms of candidiasis in all categories of patients surveyed.

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1999-09-01
2019-12-08
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References

  1. Caugant, D. A. & Sandven, P. ( 1993; ). Epidemiological analysis of Candida albicans strains by multilocus enzyme electrophoresis. J Clin Microbiol 31, 215-220.
    [Google Scholar]
  2. Dawkins, R. & Krebs, J. R. ( 1979; ). Arms races between and within species. In The Evolution of Adaptation by Natural Selection, pp. 55-77. Edited by J. M. Smith & R. Holliday. Cambridge: Cambridge University Press.
  3. Fox, J. A. , Dybdahl, M. F. , Jokela, J. & Lively, C. M. ( 1996; ). Genetic structure of coexisting sexual and clonal subpopulations in a freshwater snail ( Potamopyrgus antipodarum). Evolution 50, 1541-1548 .[CrossRef]
    [Google Scholar]
  4. Giblin, L., Edelmann, A., Maltzahn, N. B. v., Cleland, S. B. & Schmid, J. (1998). A DNA fragment associated with the success of Candida albicans strains as pathogens. Abstracts, Sixth International Mycological Congress, Jerusalem, Israel, p. 12.
  5. Gräser, Y. , Volovsek, M. , Arrington, J. , Schönian, G. , Presber, W. , Mitchell, T. G. & Vilgalys, R. ( 1996; ). Molecular markers reveal that population structure of the human pathogen Candida albicans exhibits both clonality and recombination. Proc Natl Acad Sci USA 93, 12473-12477 .[CrossRef]
    [Google Scholar]
  6. Hellstein, J. , Vawter-Hugart, H. , Fotos, P. , Schmid, J. & Soll, D. R. ( 1993; ). Genetic similarity and phenotypic diversity of commensal and pathogenic strains of Candida albicans isolated from the oral cavity. J Clin Microbiol 31, 3190-3199 .
    [Google Scholar]
  7. Hermanutz, L. A. & Weaver, S. E. ( 1996; ). Agroecotypes or phenotypic plasticity? Comparison of agrestal and ruderal populations of the weed Solanum ptycanthum. Oecologia 105, 271-280.[CrossRef]
    [Google Scholar]
  8. Hunter, P. R. ( 1991; ). The sub-specific numerical analysis of Candida albicans. J Med Vet Mycol 29, 105-115.[CrossRef]
    [Google Scholar]
  9. Jacobsen, R. & Forbes, V. E. ( 1997; ). Clonal variation in life-history traits and feeding rates in the gastropod, Potamopyrgus antipodarum: performance across a salinity gradient. Funct Ecol 11, 260-267.[CrossRef]
    [Google Scholar]
  10. Lanyon, S. M. ( 1985; ). Detecting internal inconsistencies in distance data. Syst Zool 34, 397-403.[CrossRef]
    [Google Scholar]
  11. Lapointe, F.-J. , Kirsch, J. A. W. & Bleiweiss, R. ( 1994; ). Jackknifing of weighted trees: validation of phylogenies reconstructed from distance matrices. Mol Phylogenet Evol 3, 256-267.[CrossRef]
    [Google Scholar]
  12. Odds, F. C. (1988). Candida and Candidosis, 2nd edn. London: Baillière Tindall.
  13. Ørskov, F. & Ørskov, I. ( 1983; ). Summary of a workshop on the clone concept in the epidemiology, taxonomy, and evolution of the enterobacteriaceae and other bacteria. J Infect Dis 148, 346-357.[CrossRef]
    [Google Scholar]
  14. Parker, E. D. J. & Selander, R. K. ( 1975; ). The organization of genetic diversity in the parthenogenetic lizard Cnemidophorus tesselatus. Genetics 84, 791-805.
    [Google Scholar]
  15. Parker, E. D. J. , Selander, R. K. , Hudson, R. O. & Lester, L. J. ( 1977; ). Genetic diversity in colonizing parthenogenetic cockroaches. Evolution 31, 836-842.[CrossRef]
    [Google Scholar]
  16. Pfaller, M. A. , Lockhart, S. R. , Pujol, C. , Swails-Wenger, J. A. , Messer, S. A. , Edmont, M. B. , Jones, R. N. , Wenzel, R. P. & Soll, D. R. ( 1998; ). Hospital specificity, region specificity, and fluconazole resistance of Candida albicans bloodstream isolates. J Clin Microbiol 36, 1518-1529 .
    [Google Scholar]
  17. Pujol, C. , Reynes, J. , Renaud, F. , Raymond, M. , Tibayrenc, M. , Ayala, F. J. , Janbon, F. , Mallié, M. & Bastide, J.-M. ( 1993; ). The yeast Candida albicans has a clonal mode of reproduction in a population of infected human immunodeficiency virus-positive patients. Proc Natl Acad Sci USA 90, 9456-9459 .[CrossRef]
    [Google Scholar]
  18. Pujol, C. , Joly, S. , Lockhart, S. R. , Noel, S. , Tibayrenc, M. & Soll, D. R. ( 1997; ). Parity among the randomly amplified polymorphic DNA method, multilocus enzyme electrophoresis and Southern blot hybridization with the moderately repetitive DNA probe Ca3 for fingerprinting Candida albicans. J Clin Microbiol 35, 2348-2358 .
    [Google Scholar]
  19. Schmid, J. ( 1993; ). Candidiasis: conclusions from DNA fingerprinting. Clin Adv Treatment Fungal Infect 4, 12-16.
    [Google Scholar]
  20. Schmid, J. , Voss, E. & Soll, D. R. ( 1990; ). Computer-assisted methods for assessing strain relatedness in Candida albicans by fingerprinting with the moderately repetitive sequence Ca3. J Clin Microbiol 28, 1236-1243 .
    [Google Scholar]
  21. Schmid, J. , Odds, F. C. , Wiselka, M. J. , Nicholson, K. G. & Soll, D. R. ( 1992; ). Genetic similarity and maintenance of Candida albicans strains from a group of AIDS patients, demonstrated by DNA fingerprinting. J Clin Microbiol 30, 935-941.
    [Google Scholar]
  22. Schmid, J. , Rotman, M. , Reed, B. , Pierson, C. L. & Soll, D. R. ( 1993; ). Genetic similarity of Candida albicans strains from vaginitis patients and their partners. J Clin Microbiol 31, 39-46.
    [Google Scholar]
  23. Schmid, J. , Hunter, P. R. , White, G. C. , Nand, A. K. & Cannon, R. D. ( 1995a; ). Physiological traits associated with success of Candida albicans strains as commensal colonisers and pathogens. J Clin Microbiol 33, 2920-2926 .
    [Google Scholar]
  24. Schmid, J. , Tay, Y. P. , Wan, L. , Carr, M. , Parr, D. & McKinney, W. ( 1995b; ). Evidence for nosocomial transmission of Candida albicans obtained by Ca3 fingerprinting. J Clin Microbiol 33, 1223-1230 .
    [Google Scholar]
  25. Semlitsch, R. D. , Hotz, H. & Guex, G. D. ( 1997; ). Competition among tadpoles of coexisting hemiclones of hybridogenetic Rana esculenta : support for the frozen niche variation model. Evolution 51, 1249-1261 .[CrossRef]
    [Google Scholar]
  26. Swofford, D. L. , Olsen, G. J. , Waddell, P. J. & Hillis, D. M. ( 1996; ). Phylogenetic inference. In Molecular Systematics, pp. 407-514. Edited by D. M. Hillis, C. Moritz & B. K. Mable. Sunderland, MA: Sinauer Associates.
  27. Tibayrenc, M. ( 1997; ). Are Candida albicans natural populations subdivided? Trends Microbiol 5, 253-257.[CrossRef]
    [Google Scholar]
  28. Tibayrenc, M. & Ayala, F. J. ( 1988; ). Isozyme variability in Trypanosoma cruzi, the agent of Chagas’ disease: genetical, taxonomical and epidemiological significance. Evolution 42, 277-292.[CrossRef]
    [Google Scholar]
  29. Whittam, T. S. , Ochman, H. & Selander, R. K. ( 1983; ). Multilocus genetic structure in natural populations of Escherichia coli. Proc Natl Acad Sci 80, 1751-1755 .[CrossRef]
    [Google Scholar]
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