1887

Abstract

In , Hsp18, a protein belonging to the family of small heat-shock proteins, can be detected only at high temperature. Disruption of , located upstream and in the opposite orientation to , resulted in an elevated level of mRNA at low temperature. Genetic and biochemical experiments indicated that the product of , now called RheA (epressor of ighteen), directly represses . In , an transcriptional fusion was repressed in a strain expressing RheA. DNA-binding experiments with crude extracts of overproducing RheA indicated that RheA interacts specifically with the promoter. Transcription analysis of in the wild-type and in mutant strains suggested that RheA represses transcription not only of but also of itself.

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1999-09-01
2020-04-02
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