1887

Abstract

A region of the A3(2) chromosome was identified and cloned by using as a probe the lipase gene from M11. The cloned region consisted of 6286 bp, and carried a complete lipase gene, , as well as a gene encoding a transcriptional activator (). The A3(2) gene encodes a functional extracellular lipase 82% identical to the M11 lipase; the partially purified enzyme showed a preference for substrates of short to medium chain length. Transcription of was completely dependent on the presence of , and occurred from a single promoter similar to the promoters of M11 and G. These three promoters have well-conserved −10 and −35 regions, as well as additional conserved sequences upstream of the −35 region, which could function as targets for transcriptional activation by the cognate LipR regulators. The LipR activators are related to other bacterial regulators of a similar size, constituting a previously unidentified family of proteins that includes MalT, AcoK, AlkS, AfsR, five mycobacterial proteins of unknown function and some regulators in antibiotic synthesis clusters. A lipase-deficient strain of was constructed and found to be slightly affected in production of the polyketide antibiotic actinorhodin.

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1999-09-01
2022-01-17
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