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Volume 145, Issue 9

Genetic suppression analysis of non-antibiotic-producing mutants of the locus Free

Abstract

The locus in A3(2) was identified because mutations in it uncoupled sporulation from antibiotic synthesis: mutants failed to produce any of the four antibiotics characteristic of . These mutants are now shown to contain point mutations in the gene which encodes the histidine kinase sensor-transmitter protein of a two-component signalling system. The non-antibiotic-producing mutants, which are unpigmented, spontaneously acquire pigmented colony sectors. Genetic analysis established that the pigmented sectors contain second-site suppressive mutations, (for uppressor of ). Phenotypic characterization showed that strains produce all four antibiotics; some overproduce antibiotics and are designated Pha, for recocious yperproduction of ntibiotics. A set of mutations responsible for suppression was localized by plasmid-mediated and protoplast fusion mapping techniques to the vicinity of the locus. DNA cloned from this region was used to construct phage that could transduce mutations. Sequence analysis of strains defined mutations in both the gene and the gene; the latter encodes the two-component system’s response regulator.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): Act, actinorhodin , antibiotic biosynthesis and regulation , CDA, calcium-dependent antibiotic , genetic suppression , Mmy, methylenomycin , NF, normal fertility , Red, undecylprodigiosin , Streptomyces coelicolor , two-component system and UF, ultra fertility
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1999-09-01
2024-04-19
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Genetic suppression analysis of non-antibiotic-producing mutants of the Streptomyces coelicolor absA locus
Microbiology 145, 2343 (1999); https://doi.org/10.1099/00221287-145-9-2343
/content/journal/micro/10.1099/00221287-145-9-2343
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