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Volume 145, Issue 9

Disruption of in permits expression of a heterologous α-amylase gene in the presence of glucose

The EMBL accession number for the sequence reported in this paper is AJ223365.

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Abstract

In a transposition mutant of TK24, the usually glucose-repressible expression of a heterologous α-amylase gene () became resistant to glucose repression. The transposon had inserted into an ORF called which encodes a 274 aa product sharing significant sequence similarities with various phosphatases that act on small phosphorylated substrates. was transcribed as a monocistronic mRNA and its transcription was enhanced at the transition phase. Because its transcriptional and putative translational start points coincide, is likely to be translated in the absence of a conventional RBS. The -disrupted mutant is characterized by early growth arrest in glucose-grown cultures and by partial relief of glucose repression of expression.

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Keyword(s): Amp, ampicillin , Apr, apramycin , CBS, Cibachron blue starch , glucose repression , IMP, inositol monophosphatase , induction , inositol monophosphatase , Nos, nosiheptide and transition phase
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1999-09-01
2022-01-20
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Disruption of sblA in Streptomyces lividans permits expression of a heterologous α-amylase gene in the presence of glucoseThe EMBL accession number for the sequence reported in this paper is AJ223365.
Microbiology 145, 2303 (1999); https://doi.org/10.1099/00221287-145-9-2303
/content/journal/micro/10.1099/00221287-145-9-2303
/content/journal/micro/10.1099/00221287-145-9-2303
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