1887

Abstract

The genes from A3(2) and , encoding the guanine-nucleotide exchange factor EF-Ts, were cloned and sequenced. Streptomycetes have multiple and highly divergent EF-Tu species, with EF-Tu1 and EF-Tu3 showing only about 65% amino acid sequence identity, and yet these can apparently interact with a single EF-Ts species. lies in an operon with , which encodes ribosomal protein S2. The amino acid sequence of S2 from differs from most other bacterial S2 homologues in having a C-terminal extension of 70 aa residues with a highly repetitive organization, the function of which is unknown. Transcription analysis of the operon of by promoter probing, nuclease S1 mapping and Northern blotting revealed that the genes give rise to a bicistronic transcript from a single promoter upstream of . An attenuator was identified in the intergenic region; it results in an approximately 2:1 ratio of vs transcripts. Although , encoding the major EF-Tu, is located in the ribosomal protein operon, an additional promoter in the intergenic region leads to a significant excess of EF-Tu over ribosomes. Most amino acid residues known from the crystal structure of the EF-TuEF-Ts complex to be directly involved in interaction between the two elongation factors are conserved between and . However, whenever interaction residues in the EF-Tu moiety show divergence among EF-Tu1, EF-Tu2 and EF-Tu3, the single EF-Ts exhibits compensatory substitutions of the corresponding residues. These apparently enable productive interaction to occur with all three EF-Tus.

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1999-09-01
2022-01-22
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