1887

Abstract

The gene encoding factor C (), an extracellular signal protein involved in cellular differentiation, was cloned from 45H, and the complete nucleotide sequence was determined. The deduced amino acid sequence was confirmed by HPLC/electrospray ionization-mass spectrometry analysis. The full-length protein consists of 324 amino acids and has a predicted molecular mass of 34523 Da. The mature extracellular 286 amino acid protein (31038 Da) is probably produced by cleaving off a 38 amino acid secretion signal sequence. Southern hybridization detected in several other strains, but database searches failed to identify a protein with significant homology to factor C. Expression of from a low-copy-number vector in 52-1 resulted in a phenotypic effect similar to that given by exogenously added factor C protein.

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1999-09-01
2022-01-18
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