1887

Abstract

The gene encoding factor C (), an extracellular signal protein involved in cellular differentiation, was cloned from 45H, and the complete nucleotide sequence was determined. The deduced amino acid sequence was confirmed by HPLC/electrospray ionization-mass spectrometry analysis. The full-length protein consists of 324 amino acids and has a predicted molecular mass of 34523 Da. The mature extracellular 286 amino acid protein (31038 Da) is probably produced by cleaving off a 38 amino acid secretion signal sequence. Southern hybridization detected in several other strains, but database searches failed to identify a protein with significant homology to factor C. Expression of from a low-copy-number vector in 52-1 resulted in a phenotypic effect similar to that given by exogenously added factor C protein.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-145-9-2245
1999-09-01
2020-04-05
Loading full text...

Full text loading...

/deliver/fulltext/micro/145/9/1452245a.html?itemId=/content/journal/micro/10.1099/00221287-145-9-2245&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Madden T. L., Schaffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J.. 1997; Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res25:3389–3402[CrossRef]
    [Google Scholar]
  2. Bibb M. J., Findlay P. R., Johnson M. W.. 1984; The relationship between base composition and codon usage in bacterial genes and its use for the simple and reliable identification of protein coding sequences. Gene30:157–166[CrossRef]
    [Google Scholar]
  3. Biró S., Békési, I., Vitális S., Szabó G.. 1980; A substance affecting differentiation in Streptomyces griseus. Eur J Biochem103:359–363[CrossRef]
    [Google Scholar]
  4. Chater K. F.. 1998; Taking a genetic scalpel to the Streptomyces colony. Microbiology144:1465–1478[CrossRef]
    [Google Scholar]
  5. Cheo D. L., Bayles K. W., Yasbin R. E.. 1991; Cloning and characterization of DNA damage-inducible promoter regions from Bacillus subtilis.. J Bacteriol173:1696–1703
    [Google Scholar]
  6. Cheo D. L., Bayles K. W., Yasbin R. E.. 1993; Elucidation of regulatory elements that control damage induction and competence induction of the Bacillus subtilis SOS system. J Bacteriol175:5907–5915
    [Google Scholar]
  7. Daza A., Martin J. F., Dominguez A., Gil J. A.. 1989; Sporulation of several species of Streptomyces in submerged culture after a nutritional downshift. J Gen Microbiol135:2483–2491
    [Google Scholar]
  8. von Heijne G.. 1986; A new method for predicting signal sequence cleavage sites. Nucleic Acids Res14:4683–4690[CrossRef]
    [Google Scholar]
  9. Hopwood D. A., Bibb M. J., Chater K. F., Kieser T., Bruton C. J., Lydiate D. J., Smith C. P., Ward J. M., Schrempf H.. 1985; Genetic Manipulation of Streptomyces. A Laboratory Manual Norwich: John Innes Foundation;
    [Google Scholar]
  10. Horinouchi S.. 1996; Streptomyces genes involved in aerial mycelium formation. FEMS Microbiol Lett141:1–9[CrossRef]
    [Google Scholar]
  11. Horinouchi S., Beppu T.. 1992; Autoregulatory factors and communication in Actinomycetes. Annu Rev Microbiol46:377–398[CrossRef]
    [Google Scholar]
  12. Janssen G. R., Bibb M. J.. 1993; Derivatives of pUC18 that have BglII sites flanking a modified multiple cloning site and that retain the ability to identify recombinant clones by visual screening of Escherichia coli colonies. Gene124:133–134[CrossRef]
    [Google Scholar]
  13. Kawamoto S., Ensign J. C.. 1995; Cloning and characterization of a gene involved in regulation of sporulation and cell division in Streptomyces griseus. Actinomycetes9:136–151[CrossRef]
    [Google Scholar]
  14. Kawamoto S., Watanabe H., Hesketh A., Ensign J. C., Ochi K.. 1997; Expression of the ssgA gene product, associated with sporulation and cell division in Streptomyces griseus. Microbiology143:1077–1086[CrossRef]
    [Google Scholar]
  15. Kele Z., Janáky T., Mészáros T., Fehér A, Dudits D., Szabó P. T.. 1998; Capillary chromatography/microelectrospray mass spectrometry used for the identification of putative cyclin-dependent kinase inhibitory protein in Medicago. Rapid Commun Mass Spectrom12:1564–1568[CrossRef]
    [Google Scholar]
  16. Kendrick K. E., Ensign J. C.. 1983; Sporulation of Streptomyces griseus in submerged culture. J Bacteriol155:357–366
    [Google Scholar]
  17. Khokhlov A. S.. 1958; Microbial Autoregulators Chur: Harwood Academic Publishers;
    [Google Scholar]
  18. Larson J. L., Herschberger C. L.. 1986; The minimal replicon of a streptomycete plasmid produces an ultrahigh level of plasmid DNA. Plasmid15:199–209[CrossRef]
    [Google Scholar]
  19. Lydiate D. J., Malpartida F., Hopwood D. A.. 1985; The Streptomyces plasmid SCP2*: its functional analysis and development into useful cloning vectors. Gene35:223–235[CrossRef]
    [Google Scholar]
  20. Mann M., Hojrup P., Roepstorff P.. 1993; Use of mass spectrometric molecular weight information to identify proteins in sequence database. Biol Mass Spectrom22:338–345[CrossRef]
    [Google Scholar]
  21. Mauël C, Young M., Karamata D.. 1991; Genes concerned with synthesis of poly(glycerol phosphate), the essential teichoic acid in Bacillus subtilis strain 168, are organized in two divergent transcription units. J Gen Microbiol137:929–941[CrossRef]
    [Google Scholar]
  22. Mauël C., Young M., Monsutti-Grecescu A., Marriott S. A., Karamata D.. 1994; Analysis of Bacillus subtilis tag gene expression using transcriptional fusions. Microbiology140:2279–2288[CrossRef]
    [Google Scholar]
  23. Messing J., Crea R., Seeburg P. H.. 1981; A system for shotgun DNA sequencing. Nucleic Acids Res9:309–321[CrossRef]
    [Google Scholar]
  24. Novotny M.. 1988; Recent advances in microcolumn liquid chromatography. Anal Chem60:500A–510A[CrossRef]
    [Google Scholar]
  25. Onaka H., Nakagawa T., Horinouchi S.. 1998; Involvement of two A-factor receptor homologues in Streptomyces coelicolor A3(2) in the regulation of secondary metabolism and morphogenesis. Mol Microbiol28:743–753
    [Google Scholar]
  26. Pugsley A. P.. 1993; The complete general secretory pathway in gram-negative bacteria. Microbiol Rev57:50–108
    [Google Scholar]
  27. Sambrook J., Fritsch E. F., Maniatis T.. 1989; Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  28. Sanger F., Nicklen S., Coulson A. R.. 1977; DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA74:5463–5467[CrossRef]
    [Google Scholar]
  29. Szabó G., Barabás G., Vályi-Nagy T.. 1961; Comparison of Streptomyces griseus strains which produce streptomycin and those which do not. Arch Mikrobiol40:261–274[CrossRef]
    [Google Scholar]
  30. Szabó G., Vályi-Nagy T., Vitális S.. 1962; A new factor regulating life cycle of Streptomyces griseus. In Genetics of Microorganisms, Proceedings of a Symposium on Heredity and Variability of Microorganisms pp282–292Edited by Timakova V. D.. Moscow: State Publishing House of Medical Literature;
    [Google Scholar]
  31. Szabó G., Biró S., Trón L, Valu G., Vitális S.. 1984; Mode of action of factor C upon the differentiation process of Streptomyces griseus. In Biochemical and Biomedical Aspects of Actinomycetes pp197–214Edited by Ortiz-Ortiz L., Bojalil L. F., Yakoleff V.. Orlando, FL: Academic Press;
    [Google Scholar]
  32. Szabó G., Szeszák F., Vitális S., Tóth F.. 1988; New data on the formation and mode of action of factor C. In Biology of Actinomycetes ’88 pp324–329Edited by Okami Y., Beppu T., Ogawara H.. Tokyo: Japan Scientific Societies Press;
    [Google Scholar]
  33. Szabó G., Vitális, S., Szeszák F., Biró S.. 1995; Developmental heterogeneity of Sterptomyces griseus conidia. In Sekundärmetabolismus bei Mikroorganismen pp109–122Edited by Kuhn W., Fiedler H.-P.. Tübingen: Attempto Verlag;
    [Google Scholar]
  34. Szeszák F., Szabó G.. 1973; Alteration of RNA synthesis in vitro with an endogenous regulating factor of cytodifferentiation of Streptomyces griseus. Acta Biol Acad Sci Hung24:11–17
    [Google Scholar]
  35. Szeszák F., Vitális S., Szabó G.. 1989; Factor C, a regulatory protein of Streptomyces griseus, induces release of potassium from the mycelium. J Basic Microbiol29:233–240[CrossRef]
    [Google Scholar]
  36. Szeszák F., Vitális S., Tóth F., Valu G, Fachet J., Szabó G.. 1990; Detection and determination of factor C – a regulatory protein – in Streptomyces strains by antiserum and monoclonal antibody. Arch Microbiol154:82–84
    [Google Scholar]
  37. Szeszák F., Vitális S., Szabó G.. 1991; Presence of factor C in streptomycetes and other bacteria. In Genetics and Product Formation in Streptomyces pp11–18Edited by Baumberg S., Krügel H., Noack D.. New York: Plenum;
    [Google Scholar]
  38. Szeszák F., Vitális S., Biró S., Dalmi L.. 1997; Amino acid sequence homology of factor C produced by Streptomyces griseus with regulatory proteins of zinc finger type. Acta Biol Acad Sci Hung48:265–273
    [Google Scholar]
  39. Vitális S., Szabó G.. 1969; Cytomorphological effect of factor C in submerged cultures on the hyphae of Streptomyces griseus strain No. 52-1. Acta Biol Acad Sci Hung20:85–92
    [Google Scholar]
  40. Vitális S., Szabó G., Vályi-Nagy T.. 1963; Comparison of the morphology of streptomycin producing and non-producing strains of Streptomyces griseus. Acta Biol Acad Sci Hung14:1–15[CrossRef]
    [Google Scholar]
  41. Vitális S., Biró S., Vargha G., Békési I., Szabó G.. 1981; Differentiation and its regulation in submerged culture of Streptomyces griseus. In Actinomycetes (Zentbl Bakteriol Suppl 11) pp153–156Edited by Schaal K. P., Pulverer G.. New York: Springer;
    [Google Scholar]
  42. Vitális S., Valu G., Békési I., Szeszák F., Szabó G.. 1988; Changes of protein pattern of Streptomyces griseus strains that characterize differentiation of mycelia in submerged culture. J Basic Microbiol28:393–407[CrossRef]
    [Google Scholar]
  43. Yanisch-Perron C, Vieira J., Messing J.. 1985; Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13 mp18 and pUC19 vectors. Gene33:103–119[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-145-9-2245
Loading
/content/journal/micro/10.1099/00221287-145-9-2245
Loading

Data & Media loading...

Most cited this month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error