1887

Abstract

The gene encoding factor C (), an extracellular signal protein involved in cellular differentiation, was cloned from 45H, and the complete nucleotide sequence was determined. The deduced amino acid sequence was confirmed by HPLC/electrospray ionization-mass spectrometry analysis. The full-length protein consists of 324 amino acids and has a predicted molecular mass of 34523 Da. The mature extracellular 286 amino acid protein (31038 Da) is probably produced by cleaving off a 38 amino acid secretion signal sequence. Southern hybridization detected in several other strains, but database searches failed to identify a protein with significant homology to factor C. Expression of from a low-copy-number vector in 52-1 resulted in a phenotypic effect similar to that given by exogenously added factor C protein.

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1999-09-01
2024-03-29
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References

  1. Altschul S. F., Madden T. L., Schaffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. 1997; Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402 [CrossRef]
    [Google Scholar]
  2. Bibb M. J., Findlay P. R., Johnson M. W. 1984; The relationship between base composition and codon usage in bacterial genes and its use for the simple and reliable identification of protein coding sequences. Gene 30:157–166 [CrossRef]
    [Google Scholar]
  3. Biró S., Békési, I., Vitális S., Szabó G. 1980; A substance affecting differentiation in Streptomyces griseus. Eur J Biochem 103:359–363 [CrossRef]
    [Google Scholar]
  4. Chater K. F. 1998; Taking a genetic scalpel to the Streptomyces colony. Microbiology 144:1465–1478 [CrossRef]
    [Google Scholar]
  5. Cheo D. L., Bayles K. W., Yasbin R. E. 1991; Cloning and characterization of DNA damage-inducible promoter regions from Bacillus subtilis.. J Bacteriol 173:1696–1703
    [Google Scholar]
  6. Cheo D. L., Bayles K. W., Yasbin R. E. 1993; Elucidation of regulatory elements that control damage induction and competence induction of the Bacillus subtilis SOS system. J Bacteriol 175:5907–5915
    [Google Scholar]
  7. Daza A., Martin J. F., Dominguez A., Gil J. A. 1989; Sporulation of several species of Streptomyces in submerged culture after a nutritional downshift. J Gen Microbiol 135:2483–2491
    [Google Scholar]
  8. von Heijne G. 1986; A new method for predicting signal sequence cleavage sites. Nucleic Acids Res 14:4683–4690 [CrossRef]
    [Google Scholar]
  9. Hopwood D. A., Bibb M. J., Chater K. F., Kieser T., Bruton C. J., Lydiate D. J., Smith C. P., Ward J. M., Schrempf H. 1985 Genetic Manipulation of Streptomyces. A Laboratory Manual Norwich: John Innes Foundation;
    [Google Scholar]
  10. Horinouchi S. 1996; Streptomyces genes involved in aerial mycelium formation. FEMS Microbiol Lett 141:1–9 [CrossRef]
    [Google Scholar]
  11. Horinouchi S., Beppu T. 1992; Autoregulatory factors and communication in Actinomycetes. Annu Rev Microbiol 46:377–398 [CrossRef]
    [Google Scholar]
  12. Janssen G. R., Bibb M. J. 1993; Derivatives of pUC18 that have BglII sites flanking a modified multiple cloning site and that retain the ability to identify recombinant clones by visual screening of Escherichia coli colonies. Gene 124:133–134 [CrossRef]
    [Google Scholar]
  13. Kawamoto S., Ensign J. C. 1995; Cloning and characterization of a gene involved in regulation of sporulation and cell division in Streptomyces griseus. Actinomycetes 9:136–151 [CrossRef]
    [Google Scholar]
  14. Kawamoto S., Watanabe H., Hesketh A., Ensign J. C., Ochi K. 1997; Expression of the ssgA gene product, associated with sporulation and cell division in Streptomyces griseus. Microbiology 143:1077–1086 [CrossRef]
    [Google Scholar]
  15. Kele Z., Janáky T., Mészáros T., Fehér A, Dudits D., Szabó P. T. 1998; Capillary chromatography/microelectrospray mass spectrometry used for the identification of putative cyclin-dependent kinase inhibitory protein in Medicago. Rapid Commun Mass Spectrom 12:1564–1568 [CrossRef]
    [Google Scholar]
  16. Kendrick K. E., Ensign J. C. 1983; Sporulation of Streptomyces griseus in submerged culture. J Bacteriol 155:357–366
    [Google Scholar]
  17. Khokhlov A. S. 1958 Microbial Autoregulators Chur: Harwood Academic Publishers;
    [Google Scholar]
  18. Larson J. L., Herschberger C. L. 1986; The minimal replicon of a streptomycete plasmid produces an ultrahigh level of plasmid DNA. Plasmid 15:199–209 [CrossRef]
    [Google Scholar]
  19. Lydiate D. J., Malpartida F., Hopwood D. A. 1985; The Streptomyces plasmid SCP2*: its functional analysis and development into useful cloning vectors. Gene 35:223–235 [CrossRef]
    [Google Scholar]
  20. Mann M., Hojrup P., Roepstorff P. 1993; Use of mass spectrometric molecular weight information to identify proteins in sequence database. Biol Mass Spectrom 22:338–345 [CrossRef]
    [Google Scholar]
  21. Mauël C, Young M., Karamata D. 1991; Genes concerned with synthesis of poly(glycerol phosphate), the essential teichoic acid in Bacillus subtilis strain 168, are organized in two divergent transcription units. J Gen Microbiol 137:929–941 [CrossRef]
    [Google Scholar]
  22. Mauël C., Young M., Monsutti-Grecescu A., Marriott S. A., Karamata D. 1994; Analysis of Bacillus subtilis tag gene expression using transcriptional fusions. Microbiology 140:2279–2288 [CrossRef]
    [Google Scholar]
  23. Messing J., Crea R., Seeburg P. H. 1981; A system for shotgun DNA sequencing. Nucleic Acids Res 9:309–321 [CrossRef]
    [Google Scholar]
  24. Novotny M. 1988; Recent advances in microcolumn liquid chromatography. Anal Chem 60:500A–510A [CrossRef]
    [Google Scholar]
  25. Onaka H., Nakagawa T., Horinouchi S. 1998; Involvement of two A-factor receptor homologues in Streptomyces coelicolor A3(2) in the regulation of secondary metabolism and morphogenesis. Mol Microbiol 28:743–753
    [Google Scholar]
  26. Pugsley A. P. 1993; The complete general secretory pathway in gram-negative bacteria. Microbiol Rev 57:50–108
    [Google Scholar]
  27. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  28. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467 [CrossRef]
    [Google Scholar]
  29. Szabó G., Barabás G., Vályi-Nagy T. 1961; Comparison of Streptomyces griseus strains which produce streptomycin and those which do not. Arch Mikrobiol 40:261–274 [CrossRef]
    [Google Scholar]
  30. Szabó G., Vályi-Nagy T., Vitális S. 1962; A new factor regulating life cycle of Streptomyces griseus. In Genetics of Microorganisms, Proceedings of a Symposium on Heredity and Variability of Microorganisms pp 282–292Edited by Timakova V. D. Moscow: State Publishing House of Medical Literature;
    [Google Scholar]
  31. Szabó G., Biró S., Trón L, Valu G., Vitális S. 1984; Mode of action of factor C upon the differentiation process of Streptomyces griseus. In Biochemical and Biomedical Aspects of Actinomycetes pp 197–214Edited by Ortiz-Ortiz L., Bojalil L. F., Yakoleff V. Orlando, FL: Academic Press;
    [Google Scholar]
  32. Szabó G., Szeszák F., Vitális S., Tóth F. 1988; New data on the formation and mode of action of factor C. In Biology of Actinomycetes ’88 pp 324–329Edited by Okami Y., Beppu T., Ogawara H. Tokyo: Japan Scientific Societies Press;
    [Google Scholar]
  33. Szabó G., Vitális, S., Szeszák F., Biró S. 1995; Developmental heterogeneity of Sterptomyces griseus conidia. In Sekundärmetabolismus bei Mikroorganismen pp 109–122Edited by Kuhn W., Fiedler H.-P. Tübingen: Attempto Verlag;
    [Google Scholar]
  34. Szeszák F., Szabó G. 1973; Alteration of RNA synthesis in vitro with an endogenous regulating factor of cytodifferentiation of Streptomyces griseus. Acta Biol Acad Sci Hung 24:11–17
    [Google Scholar]
  35. Szeszák F., Vitális S., Szabó G. 1989; Factor C, a regulatory protein of Streptomyces griseus, induces release of potassium from the mycelium. J Basic Microbiol 29:233–240 [CrossRef]
    [Google Scholar]
  36. Szeszák F., Vitális S., Tóth F., Valu G, Fachet J., Szabó G. 1990; Detection and determination of factor C – a regulatory protein – in Streptomyces strains by antiserum and monoclonal antibody. Arch Microbiol 154:82–84
    [Google Scholar]
  37. Szeszák F., Vitális S., Szabó G. 1991; Presence of factor C in streptomycetes and other bacteria. In Genetics and Product Formation in Streptomyces pp 11–18Edited by Baumberg S., Krügel H., Noack D. New York: Plenum;
    [Google Scholar]
  38. Szeszák F., Vitális S., Biró S., Dalmi L. 1997; Amino acid sequence homology of factor C produced by Streptomyces griseus with regulatory proteins of zinc finger type. Acta Biol Acad Sci Hung 48:265–273
    [Google Scholar]
  39. Vitális S., Szabó G. 1969; Cytomorphological effect of factor C in submerged cultures on the hyphae of Streptomyces griseus strain No. 52-1. Acta Biol Acad Sci Hung 20:85–92
    [Google Scholar]
  40. Vitális S., Szabó G., Vályi-Nagy T. 1963; Comparison of the morphology of streptomycin producing and non-producing strains of Streptomyces griseus. Acta Biol Acad Sci Hung 14:1–15 [CrossRef]
    [Google Scholar]
  41. Vitális S., Biró S., Vargha G., Békési I., Szabó G. 1981; Differentiation and its regulation in submerged culture of Streptomyces griseus. In Actinomycetes (Zentbl Bakteriol Suppl 11) pp 153–156Edited by Schaal K. P., Pulverer G. New York: Springer;
    [Google Scholar]
  42. Vitális S., Valu G., Békési I., Szeszák F., Szabó G. 1988; Changes of protein pattern of Streptomyces griseus strains that characterize differentiation of mycelia in submerged culture. J Basic Microbiol 28:393–407 [CrossRef]
    [Google Scholar]
  43. Yanisch-Perron C, Vieira J., Messing J. 1985; Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13 mp18 and pUC19 vectors. Gene 33:103–119 [CrossRef]
    [Google Scholar]
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