1887

Abstract

Cytological analysis of a series of A3(2) mutants with disruptions of early sporulation (, for white aerial mycelium) genes in an isogenic background has provided new information about the role of , and confirmed and extended previous knowledge about , and . The characteristic straight aerial hyphae of mutants contained normally spaced vegetative-like septa, while mutants in or had abnormally long and coiled aerial hyphae almost devoid of septation. , and were all absolutely required for sporulation septation, and for all visible signs of nucleoid condensation and partitioning and other changes associated with later stages of sporulation. On the other hand, appeared to enhance low basal levels of these processes. Thus, mutant aerial hyphae were divided into loosely coiled fragments of variable sizes by what appeared to be a few sporulation septa. These fragments showed some spore-like characteristics and contained condensed and aberrantly partitioned nucleoids. , and were epistatic to on the criterion that they prevented such fragments from forming in double mutants. These spore-like features and the synthesis of clearly detectable levels of the -directed grey spore pigment were not due to any residual activity of previously studied alleles since they were retained by a constructed null mutant. A model is presented that explains the mutant phenotypes by proposing two early developmental decision points involved in commitment to sporulation septation, one requiring and the other requiring and .

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1999-09-01
2019-10-13
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