1887

Abstract

The cyclic tetrapeptide HC-toxin is required for pathogenicity of the filamentous fungus on maize. HC- toxin production is controlled by a complex locus, . The isolation and characterization of a new gene of the locus, , is reported. It is shown that is specifically required for HC-toxin production and pathogenicity. It is present as two or three copies in all HC-toxin-producing (Tox2) isolates and is absent in toxin-non-producing strains. The deduced amino acid sequence of has moderate homology to many known or putative branched-chain-amino-acid transaminases from various species. A strain of with all copies of disrupted grew normally but lost HC-toxin production and pathogenicity. It is proposed that has a biosynthetic role in HC-toxin synthesis, perhaps to aminate a precursor of Aeo (2- amino-9,10-epoxi-8-oxodecanoic acid).

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1999-12-01
2019-10-14
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