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Volume 145, Issue 12

Sequences and evolutionary analyses of eukaryotic-type protein kinases from A3(2)

The GenBank accession numbers for the sequences determined in this work are AB016932, AB018799, AB019394 and AB021679.

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Abstract

Four eukaryotic-type protein serine/threonine kinases from A3(2) were cloned and sequenced. To explore evolutionary relationships between these and other protein kinases, the distribution of protein serine/threonine kinase genes in prokaryotes was examined with the program. Genes of this type were detected in only a few species of prokaryotes and their distribution was uneven; , , and each contained more than three such genes. Homology analyses by and Rdf2 programs suggested that some kinases from one species were closely related, whilst others were only remotely related. This was confirmed by examining phylogenetic trees constructed by the neighbour-joining and other methods. For each species, analysis of the coding regions indicated that the G+C content of protein kinase genes was similar to that of other genes. Considered with the fact that in phylogenetic trees the amino acid sequences of STPK from and some other eukaryotic-type protein kinases in prokaryotes form a cluster with protein kinases from eukaryotes, this suggests that the eukaryotic-type protein kinases were present originally in both prokaryotes and eukaryotes, but that most of these genes have been lost during the evolutionary process in prokaryotes because they are not needed. This conclusion is supported by the observation that the prokaryotes retaining several of these kinases undergo complicated morphological and/or biochemical differentiation.

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  • Accepted:
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  • Published Online:
Keyword(s): CaMPK, calmodulin-dependent protein kinase , cAMPPK, cAMP-dependent protein kinase , eukaryotic-type protein kinase , evolution , phylogenetic tree , protein serine/threonine kinase , S6-2PK, S6-2 protein kinase and Streptomyces
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1999-12-01
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Sequences and evolutionary analyses of eukaryotic-type protein kinases from Streptomyces coelicolor A3(2)The GenBank accession numbers for the sequences determined in this work are AB016932, AB018799, AB019394 and AB021679.
Microbiology 145, 3343 (1999); https://doi.org/10.1099/00221287-145-12-3343
/content/journal/micro/10.1099/00221287-145-12-3343
/content/journal/micro/10.1099/00221287-145-12-3343
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