1887

Microbiology

Volume 145, Issue 12

AUD4, a new amplifiable element from

The GenBank accession number for the sequence reported in this paper is AF072709.

Free

Abstract

After transformation of the chloramphenicol-sensitive, arginine-auxotrophic mutant strain AJ100 with a derivative of plasmid SCP2, some of the regenerated protoplasts contained an 82 kb DNA sequence amplified to several hundred copies per chromosome. The corresponding non-amplified sequence, called AUD4, was isolated from a λ phage genomic library of 1326. Two cytosine residues were the only directly repeated nucleotides at the ends of the element, indicating that AUD4 is a class I amplifiable sequence. The element mapped in the I-D fragment of the chromosome, where other class I amplifications have been described. The complete element was sequenced and 10 ORFs were identified. Some of the deduced proteins are highly conserved in other organisms but a putative function could be attributed to only a few of them. Duplication of AUD4 by integration of an plasmid carrying various parts of AUD4 and a thiostrepton-resistance gene in AJ100, ZX7 or TK64 induced amplification of the integrated plasmid, AUD4 or both at high frequency.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): ADS, amplified DNA sequence , AUD, amplifiable unit of DNA , class I amplifiable elements , deletion , DNA amplification , genetic instability and HT, Hickey–Tresner
© Microbiology Society
Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-145-12-3331
1999-12-01
2021-10-22
Loading full text...

Full text loading...

/deliver/fulltext/micro/145/12/1453331a.html?itemId=/content/journal/micro/10.1099/00221287-145-12-3331&mimeType=html&fmt=ahah

References

  1. Aigle B., Schneider D., Morilhat C., Vandewiele D., Dary A., Holl A.-C., Simonet J.-M., Decaris B. 1996; An amplifiable and deletable locus of Streptomyces ambofaciens RP181110 contains a very large gene homologous to polyketide synthase genes. Microbiology 142:2815–2824 [CrossRef]
     [Google Scholar]
  2. Altenbuchner J. 1993; A new λ RES vector with a built-in Tn1721-encoded excision system. Gene 123:63–68 [CrossRef]
     [Google Scholar]
  3. Altenbuchner J., Cullum J. 1985; Structure of an amplifiable DNA sequence in Streptomyces lividans 66. Mol Gen Genet 201:192–197 [CrossRef]
     [Google Scholar]
  4. Altenbuchner J., Eichenseer Ch. 1991; A new system to study DNA amplification in Streptomyces lividans. In Genetics and Product Formation in Streptomyces pp. 253–264Edited by Baumberg S., Krügel H., Noack D. New York & London: Plenum;
     [Google Scholar]
  5. Altenbuchner J., Eichenseer Ch., Brüderlein M. 1988; DNA amplification and deletion in Streptomyces lividans. In Biology of Actinomycetes pp. 139–144Edited by Okami Y. , Beppu T., Ogawara H. Tokyo: Japan Scientific Press;
     [Google Scholar]
  6. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. 1990; Basic local alignment search tool. J Mol Biol 215:403–410 [CrossRef]
     [Google Scholar]
  7. Betzler M., Tlolka I., Schrempf H. 1997; Amplification of a Streptomyces lividans 4·3 kb DNA element causes overproduction of a novel hypha- and vesicle-associated protein. Microbiology 143:1243–1252 [CrossRef]
     [Google Scholar]
  8. Chung C. T., Niemela S. L., Miller R. H. 1989; One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution. Proc Natl Acad Sci USA 86:2172–2175 [CrossRef]
     [Google Scholar]
  9. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 12:387–395 [CrossRef]
     [Google Scholar]
  10. Dharmalingam K., Cullum J. 1996; Genetic instability in Streptomyces. J Biosci 21:433–444 [CrossRef]
     [Google Scholar]
  11. Eichenseer Ch., Altenbuchner J. 1994; The very large amplifiable element AUD2 from Streptomyces lividans 66 has insertion-sequence-like repeats at its ends. J Bacteriol 176:7107–7112
     [Google Scholar]
  12. Fischer G., Decaris B., Leblond P. 1997; Occurrence of deletions, associated with genetic instability in Streptomyces ambofaciens, is independent of the linearity of the chromosomal DNA. J Bacteriol 179:4533–4558
     [Google Scholar]
  13. Fishman S. E., Hershberger C. L. 1983; Amplified DNA in Streptomyces fradiae. J Bacteriol 155:459–466
     [Google Scholar]
  14. Hopwood D. A., Kieser T., Wright H. M., Bibb M. J. 1983; Plasmids, recombination and chromosome mapping in S. lividans 66. J Gen Microbiol 129:2257–2269
     [Google Scholar]
  15. Hopwood D. A., Bibb M. J., Chater K. F.7 other authors 1985 Genetic Manipulation of Streptomyces: a Laboratory Manual Norwich, UK: John Innes Foundation;
     [Google Scholar]
  16. Hütter R., Eckhardt T. 1988; Genetic manipulation. In Actinomycetes in Biotechnology pp. 89–184Edited by Goodfellow M., Williams S. T., Modarski M. London: Academic Press;
     [Google Scholar]
  17. Karn J., Brenner S., Barnett L., Cesareni G. 1980; Novel bacteriophage cloning vector. Proc Natl Acad Sci USA 77:5172 [CrossRef]
     [Google Scholar]
  18. Katz E., Thompson C. J., Hopwood D. A. 1983; Cloning and expression of the tyrosinase gene from Streptomyces antibioticus in Streptomyces lividans. J Gen Microbiol 129:2703–2714
     [Google Scholar]
  19. Leblond P., Decaris B. 1994; New insights into the genetic instability of Streptomyces. FEMS Microbiol Lett 123:225–232 [CrossRef]
     [Google Scholar]
  20. Leblond P., Redenbach M., Cullum J. 1993; Physical map of the Streptomyces lividans 66 genome and comparison with that of the related strain Streptomyces coelicolor A3(2). J Bacteriol 175:3422–3429
     [Google Scholar]
  21. Lin Y-S., Chen C. W. 1997; Instability of artificially circularized chromosomes of Streptomyces lividans. Mol Microbiol 26:709–719 [CrossRef]
     [Google Scholar]
  22. Lin, Y.-S., Kieser H. M., Hopwood D. A., Chen C. 1993; The chromosomal DNA of Streptomyces lividans 66 is linear. Mol Microbiol 10:923–933 [CrossRef]
     [Google Scholar]
  23. Marsh J. L., Erfle M., Weykes E. J. 1984; The pIC plasmid and phage vectors with versatile cloning sites for recombinant selection by insertional inactivation. Gene 32:481–485 [CrossRef]
     [Google Scholar]
  24. Pridham T. G., Anderson P., Foly C., Linderfelser C. W., Hesseltime C. W., Benedict R. C. 1957; A selection of media for maintenance and taxonomic studies of Streptomyces. . Antibiot Annu 1956:1957947–953
     [Google Scholar]
  25. Rauland U., Glocker I., Redenbach M., Cullum J. 1995; DNA amplifications and deletions in Streptomyces lividans 66 and the loss of one end of the linear chromosome. Mol Gen Genet 246:37–44 [CrossRef]
     [Google Scholar]
  26. Redenbach M., Flett F., Piendl W., Glocker I., Rauland U., Wafzig O., Kliem R., Leblond P., Cullum J. 1993; The Streptomyces lividans 66 chromosome contains a 1 Mb deletogenic region flanked by two amplifiable regions. Mol Gen Genet 241:255–262
     [Google Scholar]
  27. Romero D., Palacios R. 1997; Gene amplification and genomic plasticity in prokaryotes. Annu Rev Genet 31:91–111 [CrossRef]
     [Google Scholar]
  28. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
     [Google Scholar]
  29. Sedlmeier R., Altenbuchner J. 1992; Cloning and DNA sequence analysis of the mercury-resistance genes of Streptomyces lividans. Mol Gen Genet 236:76–85
     [Google Scholar]
  30. Smith G. E., Summers M. D. 1980; The bidirectional transfer of DNA and RNA to nitrocellulose or diazobenzyloxymethyl paper. Anal Biochem 109:123–129 [CrossRef]
     [Google Scholar]
  31. Strohl W. R. 1992; Compilation and analysis of DNA sequences associated with apparent streptomycete promoters. Nucleic Acids Res 20:961–974 [CrossRef]
     [Google Scholar]
  32. Tsai J. F.-Y., Chen C. W. 1987; Isolation and characterization of Streptomyces lividans mutants deficient in intra-plasmid recombination. Mol Gen Genet 208:211–218 [CrossRef]
     [Google Scholar]
  33. Volff J.-N., Altenbuchner J. 1998; Genetic instability of the Streptomyces chromosome. Mol Microbiol 27:239–246 [CrossRef]
     [Google Scholar]
  34. Volff, J.-N., Eichenseer, C., Viell, P., Piendl W., Altenbuchner J. 1996; Nucleotide sequence and role in DNA amplification of the direct repeats composing the amplifiable element AUD1 of Streptomyces lividans 66. . Mol Microbiol 21:1037–1047 [CrossRef]
     [Google Scholar]
  35. Volff, J.-N., Viell P., Altenbuchner J. 1997; Artificial circularization of the chromosome with concomitant deletion of its terminal inverted repeats enhances genetic instability and genome rearrangement in Streptomyces lividans. Mol Gen Genet 253:753–760 [CrossRef]
     [Google Scholar]
  36. Yanisch-Perron C., Vieira J., Messing J. 1985; Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103–119 [CrossRef]
     [Google Scholar]
  37. Zhou X., Deng Z., Firmin J. L., Hopwood D. A., Kieser T. 1988; Site-specific degradation of Streptomyces lividans DNA during electrophoresis in buffers contaminated with ferrous iron. Nucleic Acids Res 16:4341–4352 [CrossRef]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-145-12-3331
Loading
AUD4, a new amplifiable element from Streptomyces lividansThe GenBank accession number for the sequence reported in this paper is AF072709.
Microbiology 145, 3331 (1999); https://doi.org/10.1099/00221287-145-12-3331
/content/journal/micro/10.1099/00221287-145-12-3331
/content/journal/micro/10.1099/00221287-145-12-3331
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error