1887

Abstract

After transformation of the chloramphenicol-sensitive, arginine-auxotrophic mutant strain AJ100 with a derivative of plasmid SCP2, some of the regenerated protoplasts contained an 82 kb DNA sequence amplified to several hundred copies per chromosome. The corresponding non-amplified sequence, called AUD4, was isolated from a λ phage genomic library of 1326. Two cytosine residues were the only directly repeated nucleotides at the ends of the element, indicating that AUD4 is a class I amplifiable sequence. The element mapped in the I-D fragment of the chromosome, where other class I amplifications have been described. The complete element was sequenced and 10 ORFs were identified. Some of the deduced proteins are highly conserved in other organisms but a putative function could be attributed to only a few of them. Duplication of AUD4 by integration of an plasmid carrying various parts of AUD4 and a thiostrepton-resistance gene in AJ100, ZX7 or TK64 induced amplification of the integrated plasmid, AUD4 or both at high frequency.

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1999-12-01
2020-01-28
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