1887

Microbiology

Volume 145, Issue 12

AUD4, a new amplifiable element from

The GenBank accession number for the sequence reported in this paper is AF072709.

Free

Abstract

After transformation of the chloramphenicol-sensitive, arginine-auxotrophic mutant strain AJ100 with a derivative of plasmid SCP2, some of the regenerated protoplasts contained an 82 kb DNA sequence amplified to several hundred copies per chromosome. The corresponding non-amplified sequence, called AUD4, was isolated from a λ phage genomic library of 1326. Two cytosine residues were the only directly repeated nucleotides at the ends of the element, indicating that AUD4 is a class I amplifiable sequence. The element mapped in the I-D fragment of the chromosome, where other class I amplifications have been described. The complete element was sequenced and 10 ORFs were identified. Some of the deduced proteins are highly conserved in other organisms but a putative function could be attributed to only a few of them. Duplication of AUD4 by integration of an plasmid carrying various parts of AUD4 and a thiostrepton-resistance gene in AJ100, ZX7 or TK64 induced amplification of the integrated plasmid, AUD4 or both at high frequency.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): ADS, amplified DNA sequence , AUD, amplifiable unit of DNA , class I amplifiable elements , deletion , DNA amplification , genetic instability and HT, Hickey–Tresner
© Microbiology Society
Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-145-12-3331
1999-12-01
2020-01-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/145/12/1453331a.html?itemId=/content/journal/micro/10.1099/00221287-145-12-3331&mimeType=html&fmt=ahah

References

  1. Aigle B., Schneider D., Morilhat C., Vandewiele D., Dary A., Holl A.-C., Simonet J.-M., Decaris B.. 1996; An amplifiable and deletable locus of Streptomyces ambofaciens RP181110 contains a very large gene homologous to polyketide synthase genes. Microbiology142:2815–2824[CrossRef]
     [Google Scholar]
  2. Altenbuchner J.. 1993; A new λ RES vector with a built-in Tn1721-encoded excision system. Gene123:63–68[CrossRef]
     [Google Scholar]
  3. Altenbuchner J., Cullum J.. 1985; Structure of an amplifiable DNA sequence in Streptomyces lividans 66. Mol Gen Genet201:192–197[CrossRef]
     [Google Scholar]
  4. Altenbuchner J., Eichenseer Ch.. 1991; A new system to study DNA amplification in Streptomyces lividans. In Genetics and Product Formation in Streptomyces pp.253–264Edited by Baumberg S., Krügel H., Noack D.. New York & London: Plenum;
     [Google Scholar]
  5. Altenbuchner J., Eichenseer Ch., Brüderlein M.. 1988; DNA amplification and deletion in Streptomyces lividans. In Biology of Actinomycetes pp.139–144Edited by Okami Y. , Beppu T., Ogawara H.. Tokyo: Japan Scientific Press;
     [Google Scholar]
  6. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J.. 1990; Basic local alignment search tool. J Mol Biol215:403–410[CrossRef]
     [Google Scholar]
  7. Betzler M., Tlolka I., Schrempf H.. 1997; Amplification of a Streptomyces lividans 4·3 kb DNA element causes overproduction of a novel hypha- and vesicle-associated protein. Microbiology143:1243–1252[CrossRef]
     [Google Scholar]
  8. Chung C. T., Niemela S. L., Miller R. H.. 1989; One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution. Proc Natl Acad Sci USA86:2172–2175[CrossRef]
     [Google Scholar]
  9. Devereux J., Haeberli P., Smithies O.. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res12:387–395[CrossRef]
     [Google Scholar]
  10. Dharmalingam K., Cullum J.. 1996; Genetic instability in Streptomyces. J Biosci21:433–444[CrossRef]
     [Google Scholar]
  11. Eichenseer Ch., Altenbuchner J.. 1994; The very large amplifiable element AUD2 from Streptomyces lividans 66 has insertion-sequence-like repeats at its ends. J Bacteriol176:7107–7112
     [Google Scholar]
  12. Fischer G., Decaris B., Leblond P.. 1997; Occurrence of deletions, associated with genetic instability in Streptomyces ambofaciens, is independent of the linearity of the chromosomal DNA. J Bacteriol179:4533–4558
     [Google Scholar]
  13. Fishman S. E., Hershberger C. L.. 1983; Amplified DNA in Streptomyces fradiae. J Bacteriol155:459–466
     [Google Scholar]
  14. Hopwood D. A., Kieser T., Wright H. M., Bibb M. J.. 1983; Plasmids, recombination and chromosome mapping in S. lividans 66. J Gen Microbiol129:2257–2269
     [Google Scholar]
  15. Hopwood D. A., Bibb M. J., Chater K. F..7 other authors 1985; Genetic Manipulation of Streptomyces: a Laboratory Manual Norwich, UK: John Innes Foundation;
     [Google Scholar]
  16. Hütter R., Eckhardt T.. 1988; Genetic manipulation. In Actinomycetes in Biotechnology pp.89–184Edited by Goodfellow M., Williams S. T., Modarski M.. London: Academic Press;
     [Google Scholar]
  17. Karn J., Brenner S., Barnett L., Cesareni G.. 1980; Novel bacteriophage cloning vector. Proc Natl Acad Sci USA77:5172[CrossRef]
     [Google Scholar]
  18. Katz E., Thompson C. J., Hopwood D. A.. 1983; Cloning and expression of the tyrosinase gene from Streptomyces antibioticus in Streptomyces lividans. J Gen Microbiol129:2703–2714
     [Google Scholar]
  19. Leblond P., Decaris B.. 1994; New insights into the genetic instability of Streptomyces. FEMS Microbiol Lett123:225–232[CrossRef]
     [Google Scholar]
  20. Leblond P., Redenbach M., Cullum J.. 1993; Physical map of the Streptomyces lividans 66 genome and comparison with that of the related strain Streptomyces coelicolor A3(2). J Bacteriol175:3422–3429
     [Google Scholar]
  21. Lin Y-S., Chen C. W.. 1997; Instability of artificially circularized chromosomes of Streptomyces lividans. Mol Microbiol26:709–719[CrossRef]
     [Google Scholar]
  22. Lin, Y.-S., Kieser H. M., Hopwood D. A., Chen C.. 1993; The chromosomal DNA of Streptomyces lividans 66 is linear. Mol Microbiol10:923–933[CrossRef]
     [Google Scholar]
  23. Marsh J. L., Erfle M., Weykes E. J.. 1984; The pIC plasmid and phage vectors with versatile cloning sites for recombinant selection by insertional inactivation. Gene32:481–485[CrossRef]
     [Google Scholar]
  24. Pridham T. G., Anderson P., Foly C., Linderfelser C. W., Hesseltime C. W., Benedict R. C.. 1957; A selection of media for maintenance and taxonomic studies of Streptomyces. . Antibiot Annu1956:1957–947–953
     [Google Scholar]
  25. Rauland U., Glocker I., Redenbach M., Cullum J.. 1995; DNA amplifications and deletions in Streptomyces lividans 66 and the loss of one end of the linear chromosome. Mol Gen Genet246:37–44[CrossRef]
     [Google Scholar]
  26. Redenbach M., Flett F., Piendl W., Glocker I., Rauland U., Wafzig O., Kliem R., Leblond P., Cullum J.. 1993; The Streptomyces lividans 66 chromosome contains a 1 Mb deletogenic region flanked by two amplifiable regions. Mol Gen Genet241:255–262
     [Google Scholar]
  27. Romero D., Palacios R.. 1997; Gene amplification and genomic plasticity in prokaryotes. Annu Rev Genet31:91–111[CrossRef]
     [Google Scholar]
  28. Sambrook J., Fritsch E. F., Maniatis T.. 1989; Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
     [Google Scholar]
  29. Sedlmeier R., Altenbuchner J.. 1992; Cloning and DNA sequence analysis of the mercury-resistance genes of Streptomyces lividans. Mol Gen Genet236:76–85
     [Google Scholar]
  30. Smith G. E., Summers M. D.. 1980; The bidirectional transfer of DNA and RNA to nitrocellulose or diazobenzyloxymethyl paper. Anal Biochem109:123–129[CrossRef]
     [Google Scholar]
  31. Strohl W. R.. 1992; Compilation and analysis of DNA sequences associated with apparent streptomycete promoters. Nucleic Acids Res20:961–974[CrossRef]
     [Google Scholar]
  32. Tsai J. F.-Y., Chen C. W.. 1987; Isolation and characterization of Streptomyces lividans mutants deficient in intra-plasmid recombination. Mol Gen Genet208:211–218[CrossRef]
     [Google Scholar]
  33. Volff J.-N., Altenbuchner J.. 1998; Genetic instability of the Streptomyces chromosome. Mol Microbiol27:239–246[CrossRef]
     [Google Scholar]
  34. Volff, J.-N., Eichenseer, C., Viell, P., Piendl W., Altenbuchner J.. 1996; Nucleotide sequence and role in DNA amplification of the direct repeats composing the amplifiable element AUD1 of Streptomyces lividans 66. . Mol Microbiol21:1037–1047[CrossRef]
     [Google Scholar]
  35. Volff, J.-N., Viell P., Altenbuchner J.. 1997; Artificial circularization of the chromosome with concomitant deletion of its terminal inverted repeats enhances genetic instability and genome rearrangement in Streptomyces lividans. Mol Gen Genet253:753–760[CrossRef]
     [Google Scholar]
  36. Yanisch-Perron C., Vieira J., Messing J.. 1985; Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene33:103–119[CrossRef]
     [Google Scholar]
  37. Zhou X., Deng Z., Firmin J. L., Hopwood D. A., Kieser T.. 1988; Site-specific degradation of Streptomyces lividans DNA during electrophoresis in buffers contaminated with ferrous iron. Nucleic Acids Res16:4341–4352[CrossRef]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-145-12-3331
Loading
AUD4, a new amplifiable element from Streptomyces lividansThe GenBank accession number for the sequence reported in this paper is AF072709.
Microbiology 145, 3331 (1999); https://doi.org/10.1099/00221287-145-12-3331
/content/journal/micro/10.1099/00221287-145-12-3331
/content/journal/micro/10.1099/00221287-145-12-3331
Loading

Data & Media loading...

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error