1887

Abstract

The genes and , encoding glycerol kinase and the glycerol facilitator of , a member of the Thermus/Deinococcus group, have recently been identified. The protein encoded by exhibited an unusually high degree of sequence identity (806%) when compared to the sequence of glycerol kinase from and a similar high degree of sequence identity (648%) was observed when the sequences of the glycerol facilitators of the two organisms were compared. The work presented in this paper demonstrates that is capable of taking up glycerol, that and are expressed constitutively and that glucose exerts a repressive effect on the expression of these genes. was found to possess the general components of the phosphoenolpyruvate (PEP):sugar phosphotransferase system (PTS) enzyme I and histidine-containing protein (HPr). These proteins catalyse the phosphorylation of glycerol kinase, which contains a histidyl residue equivalent to His-232, the site of PEP-dependent, PTS-catalysed phosphorylation in glycerol kinase of . Purified glycerol kinase from could also be phosphorylated with enzyme I and HPr from . Similar to enterococcal glycerol kinases, phosphorylated glycerol kinase exhibited an electrophoretic mobility on denaturing and non-denaturing polyacrylamide gels that is different from the electrophoretic mobility of non-phosphorylated glycerol kinase. However, in contrast to PEP-dependent phosphorylation of enterococcal glycerol kinases, which stimulated glycerol kinase activity about 10-fold, phosphorylation of glycerol kinase caused only a slight increase in enzyme activity.

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1999-11-01
2019-10-20
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