1887

Abstract

An insertion sequence designated IS was isolated and characterized from a clinical strain. IS was detected by high-stringency hybridization with the pMB22/S12 probe from IS of . IS is 1418 bp in size and has a G+C content of 65 mol%. It has neither terminal inverted repeats nor flanking direct repeats. Analysis of three IS insertion sites in the strain and the corresponding potential insertion sites in two IS-free strains indicated a consensus sequence of CATGCNTCCTNG for IS insertion. In the three clones examined, IS has the same orientation with respect to this target site. IS has two major ORFs. ORF1179 encodes a predicted protein of 393 amino acids. ORF930, on the complementary strand of ORF1179, encodes a protein of 310 amino acids. The Shine–Dalgarno sequence for ORF930 is partially located in the flanking region, similar to other IS-related elements. Analysis of the comparable features of insertion sequences and their variable occurrence in related organisms is useful for studying the evolution of these elements and their hosts.

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1999-11-01
2019-12-13
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