1887

Abstract

The PepR1 protein from subsp. DSM 7290 shares extensive homology with catabolite-control proteins from various Gram-positive bacteria. Expression of the subcloned gene allowed for partial complementation of a defect in . The influence of PepR1 on transcription of the prolidase gene , which is located adjacent to , was examined by use of reporter gene fusions in . PepR1 stimulated transcription initiation at the promoter about twofold, and this effect required the integrity of a 14 bp palindromic -like sequence located 74 nt upstream of . In gel-mobility-shift assays, PepR1 specifically interacted with the promoter region and also with DNA fragments covering the promoters of the , and genes of subsp. , which encode two additional peptidases and a branched-chain amino acid transporter, respectively. -like elements were identified in each of these DNA fragments. Catabolite control of PepQ was demonstrated in subsp. . During growth with lactose the enzyme activity was twofold higher than in the presence of glucose, and corresponding differences were also detected in the level of transcription.

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1999-11-01
2021-10-16
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