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Volume 145, Issue 11

Sequence analysis of three loci carrying PlcR-regulated genes encoding degradative enzymes and enterotoxin

The EMBL accession numbers for the sequences reported in this paper are given in Table 1 T1 .

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Abstract

PlcR is a pleiotropic regulator of extracellular virulence factors in the opportunistic human pathogen and the entomopathogenic , and is induced in cells entering stationary phase. Among the genes regulated by PlcR are: , encoding phosphatidylinositol-specific phospholipase C (PI-PLC); , encoding phosphatidylcholine-preferring phospholipase C (PC-PLC); , encoding the non-haemolytic enterotoxin; , encoding haemolytic enterotoxin BL (HBL); and genes specifying a putativeS-layer like surface protein and a putative extracellular RNase. By analysing 371 kb of DNA sequence surrounding , and , 28 ORFs were predicted. Three novel genes putatively regulated by PlcR and encoding a neutral protease (NprB), a subtilase family serine protease (Sfp) and a putative cell-wall hydrolase (Cwh) were identified. The corresponding and genes were located in the immediate upstream region of and could both be regulated by a putative PlcR-binding site positioned between the inversely transcribed genes. Similarly, was positioned directly upstream and transcribed in the opposite orientation to . Genes surrounding , and that were lacking an upstream PlcR regulatory sequence did not appear to serve functions apparently related to PlcR and did not exhibit a conserved organization in .

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): Bacillus cereus , HBL enterotoxin , PC-PLC, phosphatidylcholine-preferring phospholipase C , PI-PLC, phosphatidylinositol-specific phospholipase C , PlcR regulator and virulence
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1999-11-01
2024-04-23
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Sequence analysis of three Bacillus cereus loci carrying PlcR-regulated genes encoding degradative enzymes and enterotoxinThe EMBL accession numbers for the sequences reported in this paper are given in Table 1T1.
Microbiology 145, 3129 (1999); https://doi.org/10.1099/00221287-145-11-3129
/content/journal/micro/10.1099/00221287-145-11-3129
/content/journal/micro/10.1099/00221287-145-11-3129
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