1887

Abstract

PlcR is a pleiotropic regulator of extracellular virulence factors in the opportunistic human pathogen and the entomopathogenic , and is induced in cells entering stationary phase. Among the genes regulated by PlcR are: , encoding phosphatidylinositol-specific phospholipase C (PI-PLC); , encoding phosphatidylcholine-preferring phospholipase C (PC-PLC); , encoding the non-haemolytic enterotoxin; , encoding haemolytic enterotoxin BL (HBL); and genes specifying a putativeS-layer like surface protein and a putative extracellular RNase. By analysing 371 kb of DNA sequence surrounding , and , 28 ORFs were predicted. Three novel genes putatively regulated by PlcR and encoding a neutral protease (NprB), a subtilase family serine protease (Sfp) and a putative cell-wall hydrolase (Cwh) were identified. The corresponding and genes were located in the immediate upstream region of and could both be regulated by a putative PlcR-binding site positioned between the inversely transcribed genes. Similarly, was positioned directly upstream and transcribed in the opposite orientation to . Genes surrounding , and that were lacking an upstream PlcR regulatory sequence did not appear to serve functions apparently related to PlcR and did not exhibit a conserved organization in .

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1999-11-01
2019-10-20
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