aprX is a 1326 bp gene of Bacillus subtilis strain 168 that encodes a serine protease, probably intracellular, characterized by significant similarity with subtilisins, thermitases and pyrolysins. Transcription analysis, performed by RT-PCR and primer extension, allowed the localization of the active promoter and showed that aprX is expressed in stationary phase. The pattern of expression of aprX and its dependence on various transition state regulatory genes (degU, degQ, hpr, abrB, sinR), monitored by lacZ transcriptional fusions, are distinctive from those of subtilisin and other degradative enzymes. aprX is not essential for either growth or sporulation.
DevineK. M., NooneD.1998; Regulation of expression of the serine protease htrA in Bacillus subtilis. In Abstracts of the International Conference on Bacilli pp. 69Edited byYoshikawaH., SaitouH., KobayashiY., ImamuraI. Senri-Chuo, Osaka, Japan:
FerrariE., JarnaginA. S., SchmidtB. F.1993; Commercial production of extracellular enzymes. In Bacillus subtilis and Other Gram-positive Bacteria pp. 917–937Edited bySonensheinA. L., HochJ. A., LosickR. Washington, DC: American Society for Microbiology;
GlaserP., KunstF., ArnaudM.14 other authors1993; Bacillus subtilis genome project: cloning and sequencing of the 97 kb region from 325 degrees to 333 degrees. Mol Microbiol 10:371–384[CrossRef]
KoideY., NakamuraA., UozumiT., BeppuT.1986; Cloning and sequencing of the major intracellular serine protease gene of Bacillus subtilis. . J Bacteriol 167:110–116
KunstF., OgasawaraN., MoszerI.148 other authors1997; The complete genome sequence of the Gram-positive bacterium. Bacillus subtilis. Nature 390:249–256[CrossRef]
PeregoM.1993; Integrational vectors for genetic manipulations in Bacillus subtilis. In Bacillus subtilis and Other Gram-positive Bacteria pp. 615–624Edited bySonensheinA. L., HochJ. A., LosickR. Washington, DC: American Society for Microbiology;
PeroJ., SlomaA.1993; Proteases. In Bacillus subtilis and Other Gram-positive Bacteria pp. 939–952Edited bySonensheinA. L., HochJ. A., LosickR. Washington, DC: American Society for Microbiology;
ScottiC., ValbuzziA., PeregoM., GalizziA., AlbertiniA. M.1996; The Bacillus subtilis genes for ribonucleotide reductase are similar to the genes for the second class I NrdE/NrdF enzymes of Enterobacteriaceae. . Microbiology 142:2995–3004[CrossRef]
ThompsonJ. D., HigginsD. G., GibsonT. J.1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680[CrossRef]
ValbuzziA.1998Analisi genetica e funzionale dei geni nrdEF (RRasi) ed aprX (subtilasi) di Bacillus subtilis nella regione compresa tra 159 ° e 180 ° del genoma PhD thesis Università degli Studi di; Pavia: