1887

Abstract

is a 1326 bp gene of strain 168 that encodes a serine protease, probably intracellular, characterized by significant similarity with subtilisins, thermitases and pyrolysins. Transcription analysis, performed by RT-PCR and primer extension, allowed the localization of the active promoter and showed that is expressed in stationary phase. The pattern of expression of and its dependence on various transition state regulatory genes (, ), monitored by transcriptional fusions, are distinctive from those of subtilisin and other degradative enzymes. is not essential for either growth or sporulation.

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1999-11-01
2020-09-27
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