@article{mbs:/content/journal/micro/10.1099/00221287-145-10-2987, author = "Bridge, Toni A. M. and White, Chris and Gadd, Geoffrey M.", title = "Extracellular metal-binding activity of the sulphate-reducing bacterium Desulfococcus multivorans", journal= "Microbiology", year = "1999", volume = "145", number = "10", pages = "2987-2995", doi = "https://doi.org/10.1099/00221287-145-10-2987", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-145-10-2987", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", keywords = "SRB, sulphate-reducing bacteria", keywords = "Desulfococcus multivorans", keywords = "zinc binding", keywords = "metal binding", keywords = "copper binding", keywords = "sulphate-reducing bacteria", abstract = "Polarography was used to measure the copper-binding ability of culture filtrates from a range of sulphate-reducing bacteria (SRB), including pure cultures and environmental isolates. Of those tested, Desulfococcus multivorans was shown to have the greatest copper-binding capacity and this organism was used for further experiments. Extracellular copper- and zinc-binding activities of Dc. multivorans culture filtrates from batch cultures increased over time and reached a maximum after 10 d growth. The culture filtrate was shown to bind copper reversibly and zinc irreversibly. Twelve-day-old Dc. multivorans culture filtrates were shown to have a copper-binding capacity of 3·64±0·33 μmol ml−1 with a stability constant, log10 K, of 5·68±0·64 (n=4). The metal-binding compound was partially purified from culture growth media by dichloromethane extraction followed by HPLC using an acetonitrile gradient.", }