1887

Abstract

A strain of cultured in butyric acid media was found to take up phosphate following a period of phosphate release. PCR was used to clone the polyphosphate kinase () gene from the strain. The promoter for the gene was functional in the heterologous host. Using RT-PCR, transcription of the gene was found to be regulated by phosphate concentration.

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1999-10-01
2024-04-23
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References

  1. Ahn, K. & Kornberg, A. (1990). Polyphosphate kinase from Escherichia coli. J Biol Chem 265, 11734-11739. [Google Scholar]
  2. Akiyama, M. E., Crooke, E. & Kornberg, A. (1992). The polyphosphate kinase gene of Escherichia coli. Isolation and sequence of the ppk gene and membrane location of the protein. J Biol Chem 268, 633-639. [Google Scholar]
  3. Akiyama, M. E., Crooke, E. & Kornberg, A. (1993). An exopolyphosphatase of Escherichia coli. The enzyme and its ppx gene in a polyphosphate operon. J Biol Chem 268, 633-639. [Google Scholar]
  4. Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A., & Struhl, K. (1987).Current Protocols in Molecular Biology. New York: Greene Publishing Associates & Wiley Interscience.
  5. Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. Anal Biochem 72, 248-254.[CrossRef] [Google Scholar]
  6. Castuma, C. E., Huang, R., Kornberg, A. & Reusch, R. N. (1995). Inorganic polyphosphates in the aquisition of competence in Escherichia coli. J Biol Chem 270, 12980-12983.[CrossRef] [Google Scholar]
  7. Deinema, M. H., Habets, L. H. A., Scholten, J., Turksta, E. & Webers, H. A. A. M. (1980). The accumulation of polyphosphate in Acinetobacter spp. FEMS Microbiol Lett 9, 275-279.[CrossRef] [Google Scholar]
  8. Engelke, G., Gutowski-Eckel, Z., Hammelmann, M. & Entian, K.-D. (1992). Biosynthesis of the lantiobiotic nisin: genomic organization and membrane localization of the NisB protein. Appl Environ Microbiol 58, 3730-3743. [Google Scholar]
  9. Geißdörfer, W., Rarajczak, A. & Hillen, W. (1998). Transcription of ppk from Acinetobacter sp. strain ADP1, encoding a putative polyphosphate kinase, is induced by phosphate starvation. Appl Environ Microbiol 64, 896-901. [Google Scholar]
  10. van Groenestijn, J. W., Bentvelsen, M. M. A., Deinema, M. H. & Zehnder, A. J. B. (1989). Polyphosphate-degrading enzymes in Acinetobacter species and activated sludge. Appl Environ Microbiol 55, 219-223. [Google Scholar]
  11. Harold, F. M. (1966). Inorganic polyphosphate in biology: structure, metabolism and function. Bacteriol Rev 30, 772-794. [Google Scholar]
  12. Henriksen, S. D. (1976).Moraxella, Neisseria, Brahmella and Acinetobacter. Annu Rev Microbiol 30, 63-83.[CrossRef] [Google Scholar]
  13. Kato, J., Yamada, K., Muramatsu, A., Hardoyo & Ohtake, H. (1993a). Genetic improvement of Escherichia coli for enhanced biological removal of phosphate from wastewater. Appl Environ Microbiol 59, 3744–3749. [Google Scholar]
  14. Kato, J., Yamamoto, T., Yamada, K. & Ohtake, H. (1993b). Cloning, sequence and characterization of the polyphosphate-encoding gene (ppk) of Klebsiella aerogenes. Gene 137, 237-242.[CrossRef] [Google Scholar]
  15. Keasling, J. D. & Hupf, G. A. (1996). Genetic manipulation of polyphosphate metabolism affects cadmium tolerance in Escherichia coli. Appl Environ Microbiol 62, 743-746. [Google Scholar]
  16. Kornberg, A. (1995). Inorganic polyphosphate: toward making a forgotten polymer unforgettable. J Bacteriol 177, 491-496. [Google Scholar]
  17. Korstee, K. J. J., Appledoorn, K. J., Bonting, C. F. C., van Niel, E. W. J. & van Veen, H. W. (1994). Biology of polyphosphate-accumulating bacteria involved in enhanced biological phosphorus removal. FEMS Microbiol Rev 15, 137-153.[CrossRef] [Google Scholar]
  18. Kulaev, I. S. (1979).The Biochemistry of Inorganic Polyphosphates. New York: Wiley.
  19. McLean, B. W., Wiseman, S. L. & Kropinski, A. M. (1997). Functional analysis of σ-70 consensus promoters in Pseudomonas aeruginosa and Escherichia coli. Can J Microbiol 43, 981-985.[CrossRef] [Google Scholar]
  20. Martin, F. & Tolbert, N. E. (1983). Factors which affect the amount of inorganic phosphate, phosphorylcholine and phosphorylethanolamine in xylem exudate of tomato plants. Plant Physiol 73, 464-470.[CrossRef] [Google Scholar]
  21. Miller, J. H. (1972).Experiments in Molecular Genetics. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  22. Mino, T., van Loosdrecht, M. C. M. & Heijnen, J. J. (1998). Microbiology and biochemistry of the enhanced biological phosphate removal process. Water Res 11, 3193-3207. [Google Scholar]
  23. Ohtake, H., Takahashi, K., Tsuzuki, Y. & Toda, K. (1985). Uptake and release of phosphate by a pure culture of Acinetobacter calcoaceticus. Water Res 19, 1587-1594.[CrossRef] [Google Scholar]
  24. Primus, D. J. (1998).Efficient biological nutrient removal from wastewater streams in the Irish food industry. MSc thesis, National University of Ireland, Cork.
  25. Reusch, R. N. & Sadoff, H. L. (1988). Putative functions and structures of a β-hydroxybutyrate/calcium polyphosphate channel in bacterial plasma membranes. Proc Natl Acad Sci USA 85, 4176-4180.[CrossRef] [Google Scholar]
  26. Rosenberg, H., Gerdes, H. R. G. & Chegwidden, K. (1977). Two systems for the uptake of phosphate in Escherichia coli. J Bacteriol 131, 505-511. [Google Scholar]
  27. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989).Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  28. Sanger, F., Nicklen, S. & Coulson, A. R. (1977). DNA sequencing with chain-terminating inhibitors. Proc Natl Scad Sci USA 74, 5463-5467.[CrossRef] [Google Scholar]
  29. Schembri, M. A., Bayly, R. C. & Davies, J. K. (1995). Phosphate concentration regulates transcription of the Acinetobacter polyhydroxyalkanoic acid biosynthetic genes. J Bacteriol 177, 4501-4507. [Google Scholar]
  30. Torein, D. F., Gerber, A., Lotter, L. H. & Cloete, T. E. (1990). Enhanced biological phosphorus removal in activated sludge systems. Adv Microb Ecol 11, 173-230. [Google Scholar]
  31. Torriani-Gorini, A. (1994). Regulation of phosphate metabolism and transport. In Phosphate in Microorganisms, pp. 1-4. Edited by A. Torriani-Gorini, E. Yagil & S. Silver. Washington, DC: American Society for Microbiology.
  32. van Veen, H. W., Abee, T., Kortstee, G. J. J., Konings, W. N. & Zehnder, A. J. B. (1993). Characterization of two phosphate transport systems in Acinetobacter johnsonii 210A. J Bacteriol 155, 200-206. [Google Scholar]
  33. Wanner, B. L. (1996). Phosphorus assimilation and control of the phosphate regulon. In Escherichia coli and Salmonella: Cellular and Molecular Biology, 2nd edn, vol. I, pp. 1357–1381. Edited by F. C. Neidhardt and others. Washington, DC: American Society for Microbiology.
  34. Willsky, G. R. & Malamy, M. H. (1980). Characterization of two genetically separable inorganic phosphate transport systems in Escherichia coli. J Bacteriol 144, 356-365. [Google Scholar]
  35. Wood, H. G. & Clark, J. E. (1988). Biological aspects of inorganic polyphosphates. Annu Rev Biochem 57, 235-260.[CrossRef] [Google Scholar]
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