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Volume 145, Issue 10

The gene region of and the autonomous replication activities of its 5′ and 3′ flanking regions Free

Abstract

A 3·9 kb DNA fragment containing the gene region of was cloned and its nucleotide sequence was determined. Nucleotide sequence analyses indicated that this region encodes three genes in the order (ribosomal protein L34), (the putative initiator protein) and (the β subunit of DNA polymerase III). The intergenic regions between the – and – genes were found to contain several putative DnaA boxes, 9 nt long DnaA protein recognition sequences. A DNA fragment containing the 3′ but not the 5′ flanking region of the gene when cloned in plasmids, which are otherwise non-replicative in mycobacteria, exhibited autonomous replication activity in but not in BCG and . The 5′ flanking region of , on the other hand, exhibited autonomous replication activity in BCG but not in and . The implications of these results for the understanding of the replication initiation process are discussed.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): dnaA , Km, kanamycin , Mycobacterium avium , OAD, oleic acid–albumin–dextrose , oriC and replication initiation
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1999-10-01
2022-01-21
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The dnaA gene region of Mycobacterium avium and the autonomous replication activities of its 5′ and 3′ flanking regions
Microbiology 145, 2913 (1999); https://doi.org/10.1099/00221287-145-10-2913
/content/journal/micro/10.1099/00221287-145-10-2913
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