1887

Abstract

A 3·9 kb DNA fragment containing the gene region of was cloned and its nucleotide sequence was determined. Nucleotide sequence analyses indicated that this region encodes three genes in the order (ribosomal protein L34), (the putative initiator protein) and (the β subunit of DNA polymerase III). The intergenic regions between the and genes were found to contain several putative DnaA boxes, 9 nt long DnaA protein recognition sequences. A DNA fragment containing the 3′ but not the 5′ flanking region of the gene when cloned in plasmids, which are otherwise non-replicative in mycobacteria, exhibited autonomous replication activity in but not in BCG and . The 5′ flanking region of , on the other hand, exhibited autonomous replication activity in BCG but not in and . The implications of these results for the understanding of the replication initiation process are discussed.

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1999-10-01
2019-10-17
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