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Volume 145, Issue 10

Genetic organization and characteristics of the 3-(3-hydroxyphenyl)propionic acid degradation pathway of TA441 Free

Abstract

TA441 degrades 3-(3-hydroxyphenyl)propionate (3HPP) via the pathway. A gene cluster required for degradation of 3HPP was cloned from strain TA441 and sequenced. The genes encoding six catabolic enzymes, a flavin-type hydroxylase (), extradiol dioxygenase (), 2-keto-4-pentenoate hydratase (), acetaldehyde dehydrogenase (acylating) (), 4-hydroxy-2-ketovalerate aldolase () and the cleavage compound hydrolase (), were found in this cluster, encoded in this order. and were separated by two genes, and , which were not necessary for growth on 3HPP. The gene , encoding a putative transcriptional activator of the IclR family, was located adjacent to in the opposite orientation. Disruption of the or genes affected growth on 3HPP or -3-hydroxycinnamate. The and gene products showed high specificity for 3-(2,3-dihydroxyphenyl)propionate (DHPP) and the cleavage compound produced from DHPP, respectively.

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Keyword(s): 3-(3-hydroxyphenyl)propionate , 3HCI, trans-3-hydroxycinnamate , 3HPA, 3-hydroxyphenylacetate , 3HPP, 3-(3-hydroxyphenyl)propionate , 3MC, 3-methylcatechol , 4MC, 4-methylcatechol , biodegradation , CFE, cell-free extract , Comamonas testosteroni , DHBP, 2,3-dihydroxybiphenyl , DHCI, trans-2,3-dihydroxycinnamate , DHPP, 3-(2,3-dihydroxyphenyl)propionate , meta pathway and trans-3-hydroxycinnamate
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1999-10-01
2024-04-18
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Genetic organization and characteristics of the 3-(3-hydroxyphenyl)propionic acid degradation pathway of Comamonas testosteroni TA441
Microbiology 145, 2813 (1999); https://doi.org/10.1099/00221287-145-10-2813
/content/journal/micro/10.1099/00221287-145-10-2813
/content/journal/micro/10.1099/00221287-145-10-2813
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