TA441 degrades 3-(3-hydroxyphenyl)propionate (3HPP) via the pathway. A gene cluster required for degradation of 3HPP was cloned from strain TA441 and sequenced. The genes encoding six catabolic enzymes, a flavin-type hydroxylase (), extradiol dioxygenase (), 2-keto-4-pentenoate hydratase (), acetaldehyde dehydrogenase (acylating) (), 4-hydroxy-2-ketovalerate aldolase () and the cleavage compound hydrolase (), were found in this cluster, encoded in this order. and were separated by two genes, and , which were not necessary for growth on 3HPP. The gene , encoding a putative transcriptional activator of the IclR family, was located adjacent to in the opposite orientation. Disruption of the or genes affected growth on 3HPP or -3-hydroxycinnamate. The and gene products showed high specificity for 3-(2,3-dihydroxyphenyl)propionate (DHPP) and the cleavage compound produced from DHPP, respectively.


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