@article{mbs:/content/journal/micro/10.1099/00221287-145-10-2635, author = "Pujol, Claude and Joly, Sophie and Nolan, Bridgid and Srikantha, Thyagarajan and Soll, David R.", title = "Microevolutionary changes in Candida albicans identified by the complex Ca3 fingerprinting probe involve insertions and deletions of the full-length repetitive sequence RPS at specific genomic sites ", journal= "Microbiology", year = "1999", volume = "145", number = "10", pages = "2635-2646", doi = "https://doi.org/10.1099/00221287-145-10-2635", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-145-10-2635", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", keywords = "Ca3 fingerprinting", keywords = "Candida albicans", keywords = "microevolution", keywords = "RPS repetitive units", abstract = "The 11 kb complex DNA fingerprinting probe Ca3 is effective both in cluster analyses of Candida albicans isolates and in identifying microevolutionary changes in the size of hypervariable genomic fragments. A 2·6 kb EcoRI fragment of Ca3, the C fragment, retains the capacity to identify these microevolutionary changes, and when the C fragment is cleaved with SacI, the capacity is retained exclusively by a 1 kb subfragment, C1, which contains a partial RPS repeat element. The microevolutionary changes identified by Ca3, therefore, may involve reorganization of RPS elements dispersed throughout the genome. To test this possibility, hypervariable fragments from several strains of C. albicans were sequenced and compared. The results demonstrate that the microevolutionary changes identified by Ca3 are due to the insertion and deletion of full-length tandem RPS elements at specific genomic sites dispersed throughout the C. albicans genome. The RPS elements at these dispersed sites are bordered by the same upstream and downstream sequences. The frequency of recombination was estimated to be one recombination per 1000 cell divisions by following RPS reorganization in vitro. The results are inconsistent with unequal recombination between homologous or heterologous chromosomes, but consistent with intrachromosomal recombination. Two alternative models of intrachromosomal recombination are proposed: unequal sister-chromatid exchange and slipped misalignment at the replication fork.", }