1887

Abstract

The intranasal route was used to study infections in mice. Mice from two different inbred strains were challenged intranasally with and the level of local and systemic colonization was monitored. DBA/2 mice were highly susceptible to challenge and viable disseminated from the lungs to deeper tissues, including kidneys, liver and spleen within 48 h. In contrast, in BALB/c mice challenged in the same manner, were retained within the lungs and cleared. Local and systemic anti- immune responses were investigated. BALB/c mice exhibited higher titres of serum and mucosal anti- IgA than DBA/2 mice. Splenocytes from BALB/c mice, but not from DBA/2 mice, produced detectable levels of interleukin-4 and -5 following stimulation with antigens. Both DBA/2- and BALB/c-derived splenocytes produced interferon-γ and interleukin-10 in response to similar stimulation. In conclusion, the intranasal route provided a simple, non-invasive murine model for investigating infection through mucosal surfaces.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-144-8-2291
1998-08-01
2021-07-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/144/8/mic-144-8-2291.html?itemId=/content/journal/micro/10.1099/00221287-144-8-2291&mimeType=html&fmt=ahah

References

  1. Abbas A.K., Murphy K.M., Sher A. 1996; Functional diversity of helper T-lymphocytes.. Nature 383:787–793
    [Google Scholar]
  2. Ashman R.B., Kay P.H., Lynch D.M., Papadimitriou J.M. 1991; Murine candidiasis: sex differences in the severity of tissue lesions are not associated with levels of C3 and C5.. Immunol Cell Biol 69:7–10
    [Google Scholar]
  3. Ashman R.B., Bolitho E.M., Papadimitriou J.M. 1993; Patterns of resistance to Candida albicans in inbred mouse strains.. Immunol Cell Biol 71:221–225
    [Google Scholar]
  4. Barry E.M., Gomez-Duarte O., Chatfield S., Rappuoli R., Pizza M., Losonsky G., Galen J., Levine M.M. 1996; Expression and immunogenicity of pertussis toxin S1 subunit-tetanus toxin fragment C fusion in Salmonella typhi vaccine strain CVD908.. Infect Immun 64:4172–4181
    [Google Scholar]
  5. Barzu S., Fontaine A., Sansonetti P., Phalipou A. 1996; Induction of a local anti-Ipa C antibody response in mice by use of a Shigella flexneri 2a vaccine candidate: implications for use of IpaC as a protein carrier.. Infect Immun 64:1190–1196
    [Google Scholar]
  6. Bistoni F., Cenci E., Mencacci A., Schiaffella E., Mosci P., Puccetti P., Romani L. 1993; Mucosal and systemic T helper cell function after intragastric colonization of adult mice with Candida albicans.. J Infect Dis 168:1449–1457
    [Google Scholar]
  7. Cenci E., Mencacci A., Spaccapelo R., Tonnetti L., Mosci P., Enssle K.H., Puccetti P., Romani L., Bistoni F. 1995; T helper cell type 1 (Thl)-and Th2-like responses are present in mice with gastric candidiasis but protective immunity is associated with Thl development.. J Infect Dis 171:1279–1288
    [Google Scholar]
  8. Chakir J., Cote L., Coulombe C., Deslauriers N. 1994; Differential pattern of infection and immune response during experimental oral candidiasis in BALB/c and DBA/2 (H-2d) mice.. Oral Microbiol Immunol 9:88–94
    [Google Scholar]
  9. Douce G., Turcotte C., Cropley I., Roberts M., Pizza M., Domenghini M., Rappuoli R., Dougan G. 1995; Mutants of E. coli heat-labile toxin lacking ADP-ribosyltransferase activity act as nontoxic mucosal adjuvants.. Proc Natl Acad Sci USA 92:1644–1648
    [Google Scholar]
  10. Dupont P.F. 1995; Candida albicans, the opportunist. A cellular and molecular perspective.. J Am Podiatr Med Assoc 85:104–115
    [Google Scholar]
  11. Elson C.O., Ealding W., Lefkowitz J. 1984; A lavage technique allowing repeated measurement of IgA antibody in mouse intestinal secretions.. J Immunol Methods 67:101–108
    [Google Scholar]
  12. Fallon K., Bausch K., Noonan J., Huguenel E., Tamburini P. 1997; Role of aspartic proteases in disseminated Candida albicans infection in mice.. Infect Immun 65:551–556
    [Google Scholar]
  13. Fedorka-Cray P.J., Kelley L.C., Stabel T.J., Gray J.T., Laufer J.A. 1995; Alternate routes of invasion may affect pathogenesis of Salmonella typhimurium in swine.. Infect Immun 63:2658–2664
    [Google Scholar]
  14. Galen J.E., Gomez-Duarte O.G., Losonsky G.A., Halpern J.L, Lauderbaugh C.S., Kaintuck S., Reymann M.K., Levine M.M. 1997; A murine model of intranasal immunisation to assess the immunogenicity of attenuated Salmonella typhi live vector vaccines in stimulating serum antibody responses to expressed foreign antigens.. Vaccine 15:700–708
    [Google Scholar]
  15. Gray J.T., Fedorka-Cray P.J., Stabel T.J., Ackermann M.R. 1995; Influence of inoculation route on the carrier state of Salmonella choleraesuis in swine.. Vet Microbiol 47:43–59
    [Google Scholar]
  16. Greenfield R.A., Joyce W.A. 1993; Gastric colonization with Candida albicans.. Mycopathologia 122:1–5
    [Google Scholar]
  17. Hector R.F., Domer J.E., Carrow E.W. 1982; Immune responses to Candida albicans in genetically distinct mice.. Infect Immun 38:1020–1028
    [Google Scholar]
  18. Helstrom P.B., Balish E. 1979; Effect of oral tetracycline, the microbial flora, and the athymic state on gastrointestinal colonization and infection of BALB/c mice with Candida albicans.. Infect Immun 23:764–774
    [Google Scholar]
  19. Hurley R. 1966; Effect of route of entry of Candida albicans on the histogenesis of the lesions in experimental candidosis in the mouse.. J Pathol Bacteriol 92:578–583
    [Google Scholar]
  20. Jensen J., Warner T., Balish E. 1993; Resistance of SCID mice to Candida albicans administered intravenously or colonizing the gut: role of polymorphonuclear leukocytes and macrophages.. J Infect Dis 167:912–919
    [Google Scholar]
  21. Jensen J., Warner T., Balish E. 1994; The role of phagocytic cells in resistance to disseminated candidiasis in granulocytopenic mice.. J Infect Dis 170:900–905
    [Google Scholar]
  22. Jensen J., Warner T., Johnson C., Balish E. 1996; Oral immunization of mice against candidiasis.. J Infect Dis 174:133–140
    [Google Scholar]
  23. Klein R.S., Harris C.A., Small C.B., Moll B., Lesser M., Friedland G.H. 1984; Oral candidiasis in high risk patients as the initial manifestation of the acquired immunodeficiency syndrome.. N Engl J Med 311:354–358
    [Google Scholar]
  24. Klingspor L., Stintzing G., Fasth A., Tollemar J. 1996; Deep Candida infection in children receiving allogeneic bone marrow transplants: incidence, risk factors and diagnosis.. Bone Marrow Transplant 17:1043–1049
    [Google Scholar]
  25. Lacasse M., Fortier C., Chakir J., Cote L., Deslauriers N. 1993; Acquired resistance and persistence of Candida albicans following oral candidiasis in the mouse: a model of the carrier state in humans.. Oral Microbiol Immunol 8:313–318
    [Google Scholar]
  26. Mallett C.P., Van de Verg L., Collins H.H., Hale T.L. 1993; Evaluation of Shigella vaccine safety and efficacy in an intranasally challenged mouse model.. Vaccine 11:190–196
    [Google Scholar]
  27. Mallett C.P., Hale T.L., Kaminski R.W., Larsen T., Orr N., Cohen D., Lowell G.H. 1995; Intranasal or intragastric immunisation with proteosome-Shigella lipopolysaccharide vaccines protects against lethal pneumonia in a murine model of Shigella infection.. Infect Immun 63:2382–2386
    [Google Scholar]
  28. Noriega F.R., Losonsky G., Lauderbaugh C., Liao F.M., Wang J.Y., Levine M. 1996; Engineered △guaBAvirG Shigella flexneri 2a strain CVD1205: construction, safety, immunogenicity and potential efficacy as a mucosal vaccine.. Infect Immun 64:3055–3061
    [Google Scholar]
  29. Nuget K.M., Onofrio J.M. 1983; Pulmonary tissue resistance to Candida albicans in normal and immunosuppressed mice.. Am Rev Respir Dis 128:909–914
    [Google Scholar]
  30. Papadimitriou J.M., Ashman R.B. 1986; The pathogenesis of acute systemic candidiasis in a susceptible inbred mouse strain.. J Pathol 150:257–265
    [Google Scholar]
  31. Pfaller M.A. 1996; Nosocomial candidiasis: emerging species, reservoirs, and modes of transmission.. Clin Infect Dis 22:S89–S94
    [Google Scholar]
  32. Phalipon A., Kaufmann M., Michetti P., Cavaillon J., Huerre M., Sansonetti P., Kraehenbuhl J.P. 1995; Monoclonal immunoglobulin A antibody directed against serotype-specific epitope of Shigella flexneri lipopolysaccharide protects against murine experimental shigellosis.. J Exp Med 182:769–778
    [Google Scholar]
  33. Romani L., Mencacci A., Cenci E., Spaccapelo R., Mosci P., Puccetti P., Bistoni F. 1993; CD4+ subset expression in murine candidiasis. Th responses correlate directly with genetically determined susceptibility or vaccine-induced resistance.. J Immunol 150:925–931
    [Google Scholar]
  34. Romani L., Cenci E., Menacci A., Bistoni F., Puccetti P. 1995; T helper cell dichotomy to Candida albicans: implications for pathology, therapy, and vaccine design.. Immunol Res 14:148–162
    [Google Scholar]
  35. Roof M.B., Doitchinoff D.D. 1995; Safety, efficacy and duration of immunity induced in swine by use of an avirulent live Salmonella choleraesuis-containing vaccine.. Am J Vet Res 56:39–44
    [Google Scholar]
  36. Samaranayake L.P., Holmstrop P. 1989; Oral candidiasis and human immunodeficiency virus infection.. J Oral Pathol Med 18:554–564
    [Google Scholar]
  37. Samonis G., Dassiou M., Anastassiadou H. 1994; Antibiotics affecting colonisation of mice by yeasts.. J Chemother 6:50–52
    [Google Scholar]
  38. Sawyer R.T. 1990; Experimental pulmonary candidiasis.. Mycopathologia 109:99–109
    [Google Scholar]
  39. Sizemore D.R., Bramstrom A.A., Sadoff J.C. 1997; Attenuated bacteria as a DNA delivery vehicle for DNA-mediated immunisation.. Vaccine 15:804–807
    [Google Scholar]
  40. Stabel T.J., Fedorka-Cray P.J., Gray J.T. 1995; Tumour necrosis factor-α production in swine after oral or respiratory challenge exposure with live Salmonella typhimurium or Salmonella choleraesuis.. Am J Vet Res 56:1012–1018
    [Google Scholar]
  41. van de Verg L.L., Mallet C.P., Collins H.H., Larsen T., Hammack C., Hale T.L. 1995; Antibody and cytokine response in a mouse pulmonary model of Shigella flexneri serotype 2a infection.. Infect Immun 63:1947–1954
    [Google Scholar]
  42. Voino-Yasenetsky M.V., Voino-Yasenetsky M.K. 1962; Experimental pneumonia caused by bacteria of the Shigellagroup.. Acta Morphol Acad Sci Hung 11:439–454
    [Google Scholar]
  43. Welkos S., O̓Brien A. 1994; Determination of median lethal and infectious doses in animal model systems.. In Bacterial Pathogenesis pp. 29–39 Clark V.L., Bavoil P.M. Edited by London:: Academic Press;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-144-8-2291
Loading
/content/journal/micro/10.1099/00221287-144-8-2291
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error