1887

Abstract

The effect of manganous ions [Mn(II)] and ferrous ions [Fe(II)] on expression of photosynthesis genes in was investigated. The presence of Mn(II) during phototrophic (anaerobic) and chemotrophic (aerobic) growth of caused a decrease in the amount of bacteriochlorophyll and carotenoid pigments which were synthesized and this was associated mainly with a decrease in the level of light-harvesting complex II. Mn(II) was shown to cause a decrease in expression of the operon, which encodes the polypeptides of light-harvesting complex II. Expression of the operon is controlled by the central repressor of photosynthesis gene expression, PpsR. In a mutant there was no effect of Mn(II) on photosynthesis gene expression. It is concluded that Mn(II) may act as a compressor in the action of PpsR or act via an as yet uncharacterized protein that interacts with PpsR. In contrast to the effects of Mn(II), Fe(II) was required for high levels of photosynthesis gene expression. This requirement for Fe(II) was shown to be related to the regulation of , a gene under the control of the transcriptional regulator, FnrL. Mn(II) did not affect FnrL-dependent gene expression.

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1998-08-01
2024-04-25
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References

  1. Aagaard J., Sistrom W.R. 1972; Control of synthesis of reaction center bacteriochlorophyll in Rhodopseudomonas sphaeroides.. Photochem Photobiol 15:209–225
    [Google Scholar]
  2. Beinert H., Kiley P. 1996; Redox control of gene expression involving iron-sulfur proteins. Change of oxidation-state or assembly/disassembly of Fe-S clusters?. FEBS Lett 382:218–219
    [Google Scholar]
  3. Bonnett T.C., Cobine P., Sockett R.E., McEwan A.G. 1995; Phenotypic characterization and genetic complementation of dimethylsulfoxide respiratory mutants of Rhodobacter sphaeroides and Rhodobacter capsulatus.. FEMS Microbiol Lett 133:163–168
    [Google Scholar]
  4. Boyd J., Oza M.N., Murphy J.R. 1990; Molecular cloning and DNA sequence of a diphtheria tox iron-dependent regulatory element (dtxR) from Corynebacterium diphtheriae.. Proc Natl Acad Sci USA 87:5968–5972
    [Google Scholar]
  5. Burke D.H., Alberti M., Hearst J.E. 1993; The Rhodobacter capsulatus chlorin reductase-encoding locus, bchA, consists of three genes, bchX, bchY and bchZ.. J Bacterial 175:2407–2413
    [Google Scholar]
  6. Burnham B.F., Lascelles J. 1963; Control of porphyrin biosynthesis through a negative feedback mechanism.. Biochem J 87:462–472
    [Google Scholar]
  7. Chen L., Helmann J.D. 1995; Bacillus subtilis MrgA is a Dps (PexB) homologue: evidence for metalloregulation of oxidativestress gene.. Mol Microbiol 18:295–300
    [Google Scholar]
  8. Chen L., James L.P., Helmann J.D. 1993; Metalloregulation in Bacillus subtilis: isolation and characterization of two genes differentially repressed by metal ions.. J Bacterial 175:5428–5437
    [Google Scholar]
  9. Chen L., Keramati L., Helmann J.D. 1995; Coordinate regulation of Bacillus subtilis peroxide stress genes by hydrogen peroxide and metal ions.. Proc Natl Acad Sci USA 92:8190–8194
    [Google Scholar]
  10. Clark J.M. Jr Switzer R.L. 1977 Experimental Biochemistry p. 97 New York: W. H. Freeman;
    [Google Scholar]
  11. Clayton R.K. 1970 Light and Living Matter, vol. 1 The Physical Part. New York: McGraw-Hill;
    [Google Scholar]
  12. Eraso J.M., Kaplan S. 1994; prrA, a putative response regulator involved in oxygen regulation of photosynthesis gene expression in Rhodobacter sphaeroides.. J Bacteriol 176:32–43
    [Google Scholar]
  13. Eraso J.M., Kaplan S. 1995; Oxygen-insensitive synthesis of the photosynthetic membranes of Rhodobacter sphaeroides: a mutant histidine kinase.. J Bacteriol 177:2695–2706
    [Google Scholar]
  14. Ernst J.F., Bennett R.L., Rothfield L.I. 1978; Constitutive expression of the iron-enterochelin and ferrichrome uptake systems in a mutant strain of Salmonella Typhimurium.. J Bacteriol 135:928–934
    [Google Scholar]
  15. Gomelsky M., Kaplan S. 1995a; Genetic evidence that PpsR from Rhodobacter sphaeroides 2.4.1 functions as a repressor of puc and bchF expression.. J Bacteriol 177:1634–1637
    [Google Scholar]
  16. Gomelsky M., Kaplan S. 1995b; appA, a novel gene encoding a trans-acting factor involved in the regulation of photosynthesis gene expression in Rhodobacter sphaeroides 2.4.1.. J Bacteriol 172:4609–4618
    [Google Scholar]
  17. Gomelsky M., Kaplan S. 1997; A molecular genetic analysis suggesting interactions between AppA and PpsR in the regulation of photosynthesis gene expression in Rhodobacter sphaeroides 2.4.1.. J Bacteriol 179:128–134
    [Google Scholar]
  18. Hantke K. 1987; Selection procedure for deregulated iron transport mutants (fur) in Escherichia coli K12: fur not only affects iron metabolism.. Mol Gen Genet 210:135–139
    [Google Scholar]
  19. Hennecke H. 1990; Regulation of bacterial gene expression by metal-protein complexes.. Mol Microbiol 4:1621–1628
    [Google Scholar]
  20. Hickey E.K., Ciancotto N.P. 1994; Cloning and sequencing of the Legionella pneumophila fur gene.. Gene 143:117–121
    [Google Scholar]
  21. Horne I.M., Pemberton J.M., McEwan A.G. 1996; Photo-synthesis gene expression in Rhodobacter sphaeroides is regulated by redox changes which are linked to electron transport.. Microbiology 142:2831–2838
    [Google Scholar]
  22. Kassner R.J., Kamen M.D. 1968; Trace metal composition of photosynthetic bacteria.. Biochim Biophys Acta 153:270–278
    [Google Scholar]
  23. Kiley P.J., Kaplan S. 1988; Molecular genetics of photo-synthetic membrane biosynthesis in Rhodobacter sphaeroides.. Microbiol Rev 52:50–69
    [Google Scholar]
  24. Lascelles J. 1959; Adaptation to form bacteriochlorophyll in Rhodopseudomonas sphaeroides: changes in activity of enzymes concerned in pyrrole synthesis.. Biochem J 72:508–518
    [Google Scholar]
  25. Lazazzera B.A., Beinert H., Khoroshilova N., Kennedy M.C., Kiley P.J. 1996; DNA binding and dimerization of the Fe-S-containing FNR protein from Escherichia coli are regulated by oxygen.. J Biol Chem 271:2762–2768
    [Google Scholar]
  26. Lee J.K., Kaplan S. 1992; cis-acting regulatory elements involved in oxygen regulation of puc operon transcription in Rhodobacter sphaeroides.. J Bacteriol 174:1158–1171
    [Google Scholar]
  27. McEwan A.G. 1994; Photosynthetic electron transport and anaerobic metabolism in purple non-sulfur bacteria.. Antonie Leeuwenhoek 66:151–164
    [Google Scholar]
  28. Meinhardt S.W., Kiley P.J., Kaplan S., Crofts A.R., Harayama S. 1984; Characterization of light-harvesting mutants of Rhodopseudomonas sphaeroides. I. Measurement of the efficiency of energy transfer from the light-harvesting complexes to the reaction centre.. Arch Biochem Biophys 236:130–139
    [Google Scholar]
  29. Niehaus F., Hantke K., Unden G. 1991; Iron content and FNR-dependent gene regulation in Escherichia coli.. FEMS Microbiol Lett 84:319–324
    [Google Scholar]
  30. Pemberton J.M., Bowen A.R.St G. 1981; High frequency chromosome transfer in Rhodopseudomonas sphaeroides promoted by the broad host range plasmid RP1 carrying the mercury transposon Tn501.. J Bacteriol 161:469–472
    [Google Scholar]
  31. Penfold R.J., Pemberton J.M. 1991; A gene from the photosynthetic gene cluster of Rhodobacter sphaeroides induces trans suppression of bacteriochlorophyll and carotenoid levels in R. sphaeroides and R. capsulatus.. Curr Microbiol 23:259–263
    [Google Scholar]
  32. Penfold R.J., Pemberton J.M. 1994; Sequencing, chromo-somal inactivation and functional expression in Escherichia coli of ppsR, a gene which represses carotenoid and bacteriochlorophyll synthesis in Rhodobacter sphaeroides.. J Bacteriol 176:2869–2876
    [Google Scholar]
  33. Ponnampalam S.N., Bauer C.E. 1997; DNA binding characteristics of CrtJ.. J Biol Chem 272:18391–18396
    [Google Scholar]
  34. Ponnampalam S.N., Buggy J.J., Bauer C.E. 1995; Purification and characterization of the aerobic repressor CrtJ that coordinately regulates bacteriochlorophyll, carotenoid and light-harvesting II expression in Rhodobacter capsulatus.. In Abstracts of the Diversity, Genetics and Physiology of Photosynthetic Prokaryotes, Indiana University Bloomington, Indiana, USA abstract 37
    [Google Scholar]
  35. Rouault T.A., Klausner R.D. 1996; Iron-sulfur clusters as biosensors of oxidants and iron.. Trends Biochem Sci 21:174–177
    [Google Scholar]
  36. Sambrook J., Fritsch E.F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2nd edn.. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  37. Simon R., Priefer U., Puhler A. 1983; A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram-negative bacteria.. Bio/Technology 1:784–791
    [Google Scholar]
  38. Smith P.K., Krohn R.I., Hermanson G.T. 7 other authors 1985; Measurement of protein using bicinchoninic acid.. Anal Biochem 150:76–85
    [Google Scholar]
  39. Staggs T.M., Perry R.D. 1992; Fur regulation in Yersinia species.. Mol Microbiol 6:2507–2516
    [Google Scholar]
  40. Trageser M., Unden G. 1989; Role of cysteine residues and of metal ions in the regulatory functioning of FNR, the transcriptional regulator of anaerobic respiration in Escherichia coli.. Mol Microbiol 3:593–599
    [Google Scholar]
  41. Unden G., Becker S., Bongaerts J., Holighaus G., Schirawaski J., Six S. 1995; O2-sensing and O2-dependent gene regulation in facultatively anaerobic bacteria.. Arch Microbiol 164:81–90
    [Google Scholar]
  42. Weaver P.F., Wall J.D., Gest H. 1975; Characterization of Rhodopseudomonas capsulata.. Arch Microbiol 105:207–216
    [Google Scholar]
  43. Williams R.J.P. 1982; Free manganese (II) and iron (II) cations can act as intracellular cell controls.. FEBS Lett 140:3–10
    [Google Scholar]
  44. Zeilstra-Ryalls J.H., Kaplan S. 1995; Aerobic and anaerobic regulation in Rhodobacter sphaeroides 2.4.1: the role of the fnrL gene.. J Bacteriol 177:6422–6431
    [Google Scholar]
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