1887

Abstract

A novel filamentous bacteriophage, fs-2, was isolated from Vibrio cholerae O139 strain MDO14. The fs-2 phage was a long filamentous particle 1200 nm long and 7 nm wide. The purified phage formed a turbid plaque when spotted on a lawn of the host organisms. The plaque-formation activity was stable following heating to 70 � but was inhibited by treatment with chloroform. fs-2 had a single-stranded DNA genome and was converted to a double-stranded replicative form in the host cell. Almost all V. cholerae O139 and O1 El Tor biotype strains tested were sensitive to the phage, but most O1 classical strains and non-O1 non-O139 strains were resistant. The fs-2 genome comprised 8651 nucleotides containing nine open reading frames, five of which had predicted protein products partially homologous to the reported protein products of other filamentous phages. Although the extent of the homology was not particularly high, the genetic organization of other filamentous phages appears to be preserved in fs-2. The phage was not integrated into the chromosome of its host, but a 715 nucleotide fragment located in the large intergenic region of fs-2 was highly homologous to a part of region RS2 (repetitive sequence 2) of the V. cholerae CTX sequence which is speculated to be required for integration of the phage into the V. cholerae chromosome at a specific site.

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1998-07-01
2024-04-23
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References

  1. Altschul S.F., Gish W., Miller W., Myers E.W., Lipman D.J. 1990; Basic local alignment search tool.. J Mol Biol 215:403–410
    [Google Scholar]
  2. Beck E., Zink B. 1981; Nucleotide sequence and genome organization of filamentous bacteriophages fl and fd.. Gene 16:35–58
    [Google Scholar]
  3. Birnboim H.C., Doly J. 1979; A rapid alkaline extraction procedure for recombinant plasmid DNA.. Nucleic Acids Res 7:1513–1523
    [Google Scholar]
  4. Ehara M., Shimodori S., Kojima F., Ichinose Y., Hirayama T., Albert M.J., Supawat K., Honma Y., Iwanaga M., Amako K. 1997; Characterization of filamentous phage of Vibrio cholerae0139 and O1.. FEMS Microbiol Lett 154:293–301
    [Google Scholar]
  5. Esposito D., Fitzmaurice W.P., Benjamin R.C., Goodman S.D., Waldman A.S., Scocca J.J. 1996; The complete nucleotide sequence of bacteriophage HP1 DNA.. Nucleic Acids Res 12:2360–2368
    [Google Scholar]
  6. Kar S., Ghosh R.K., Ghosh A.N., Ghosh A. 1996; Integration of the DNA of a novel filamentous bacteriophage VSK from Vibrio cholerae 0139 into the host chromosomal DNA.. FEMS Microbiol Lett 145:17–22
    [Google Scholar]
  7. Laemmli U.K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4.. Nature 227:680–685
    [Google Scholar]
  8. Luiten R.G.M., Putterman D.G., Schoenmakers J.G.G., Konings R.N.H., Day L.A. 1985; Nucleotide sequence of the genome of Pf3, an IncP-1 plasmid-specific filamentous bacteriophage of Pseudomonas aeruginosa.. J Virol 56:268–276
    [Google Scholar]
  9. McMaster G.K., Carmichael G.G. 1977; Analysis of single and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.. Proc Natl Acad Sci USA 744835–4838
    [Google Scholar]
  10. Model P., Russel M. 1988; Filamentous bacteriophage.. In The Bacteriophages pp. 375–456 Calender R. Edited by New York:: Plenum.;
    [Google Scholar]
  11. Peeters B.P.H., Peters R.M., Schoenmakers J.G.G., Konings R.N.H. 1985; Nucleotide sequence and genetic organization of the genome of the N-specific filamentous bacteriophage IKe. Comparison with the genome of the F-specific filamentous phages M13, fd and fl.. J Mol Biol 181:27–39
    [Google Scholar]
  12. Rammanurthy T., Garg S., Sharma R. 8 other authors 1993; Emergence of novel strain of Vibrio cholerae with epidemic potential in southern and eastern India.. Lancet 341:703–704
    [Google Scholar]
  13. Rowe B., Frost J.A. 1992; Vibrio phages and phage-typing.. In Cholera pp. 95–105 Dhihman B., William B.G. Edited by New York:: Plenum.;
    [Google Scholar]
  14. Sambrook J., Fritsch E.F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2nd edn.. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  15. Sanger F., Nicklen S., Coulson A.R. 1977; DNA sequencing with chain-terminating inhibitors.. Proc Natl Acad Sci USA 745463–5467
    [Google Scholar]
  16. Shimodori S., lida K., Kojima F., Takeda A., Ehara M., Amako K. 1997; Morphological features of a filamentous phage of Vibrio cholerae 0139 Bengal.. Microbiol Immunol 41:757–763
    [Google Scholar]
  17. Stassen A.P., Schoenmakers E.F., Yu M., Schoenmakers J.G., Konings R.N. 1992; Nucleotide sequence of the genome of the filamentous bacteriophage 12-2: module evolution of the filamentous phage genome.. J Mol Evol 34:141–152
    [Google Scholar]
  18. Taniguchi H., Sato K., Ogawa M., Udou T., Mizuguchi Y. 1984; Isolation and characterization of a filamentous phage, Vf33, specific for Vibrio parahaemolyticus.. Microbiol Immunol 28:327–337
    [Google Scholar]
  19. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.. Proc Natl Acad Sci USA 764350–4354
    [Google Scholar]
  20. Tseng Y.-H., Lo M.-C, Lin K.-C., Pan C.-C., Chang R.-Y. 1990; Characterization of filamentous bacteriophage ɸLf from Xanthomonas campestris pv. campestris.. J Gen Virol 71:1881–1884
    [Google Scholar]
  21. Van Wenzenbeek P.M.G.F., Hulsebos T.J.M., Schoenmakers J.G.G. 1980; Nucleotide sequence of the filamentous bacteriophage M13 DNA genome: comparison with phage fd.. Gene 11:129–148
    [Google Scholar]
  22. Von Heijne G. 1985; Signal sequences. The limits of variation.. J Mol Biol 184:99–105
    [Google Scholar]
  23. Waldor M.K., Mekalanos J.J. 1996; Lysogenic conversion by a filamentous phage encoding cholera toxin.. Science 272:1910–1914
    [Google Scholar]
  24. Waldor M.K., Rubin E.J., Pearson G.D., Kimsey H., Mekalanos J.J. 1997; Regulation, replication, and integration function of the Vibrio cholerae CTXɸ are encoded by region RS2.. Mol Microbiol 24:917–926
    [Google Scholar]
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