1887

Abstract

A single Borrelia burgdorferi bacterium may contain six or more different 32 kb circular plasmids (cp32s). Although these plasmids are homologous throughout much of their sequences, two loci have been identified at which they can vary significantly. The cp32 plasmids and their relatives each contain two adjacent genes, orfC and orf3, that vary in sequence between plasmids found within clones of individual bacteria. The orfC gene product is homologous to proteins involved in partitioning of bacterial plasmids, and the differences at this locus between plasmids may account for their compatibility. The orfC-orf3 loci are located approximately 5 kb from another variable locus called erp. The orfC-orf3 loci were used as physically linked markers to assess genetic rearrangements in the erp loci; this revealed examples of recombination involving both individual genes and entire erp loci. Recombination of the genes encoding the Erp antigens might contribute to the evasion of the mammalian immune response and could play roles in the establishment and persistence of B. burgdorferi infections in mammalian hosts.

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1998-07-01
2021-07-28
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References

  1. Abeles A.L, Snyder K.M., Chattoraj D.K. 1984; PI plasmid replication: replicon structure.. J Mol Biol 173:307–324
    [Google Scholar]
  2. Akins D.R., Porcella S.F., Popova T.G., Shevchenko D., Baker S.I., Li M., Norgard M.V. 1995; Evidence for in vivo but not in vitro expression of a Borrelia burgdorferi outer surface protein F (OspF) homologue.. Mol Microbiol 18:507–520
    [Google Scholar]
  3. Amouriaux P., Assous M., Margarita D., Baranton G., SaintGirons I. 1993; Polymerase chain reaction with the 30-kb circular plasmid of Borrelia burgdorferi B31 as a target for detection of the Lyme borreliosis agents in cerebrospinal fluid.. Res Microbiol 144:211–219
    [Google Scholar]
  4. Austin S., Abeles A. 1983a; Partition of unit-copy mini-plasmids to daughter cells. I. PI and F miniplasmids contain discrete, interchangeable sequences sufficient to promote equi- partition.. J Mol Biol 169:353–372
    [Google Scholar]
  5. Austin S., Abeles A. 1983b; Partition of unit-copy mini-plasmids to daughter cells. II. The partition region of miniplasmid PI encodes an essential protein and a centromere-like site at which it acts.. J Mol Biol 169:373–387
    [Google Scholar]
  6. Barbour A.G. 1988; Plasmid analysis of Borrelia burgdorferi, the Lyme disease agent.. J Clin Microbiol 26:475–478
    [Google Scholar]
  7. Barbour A.G., Carter C.J., Bundoc V., Hinnebusch J. 1996; The nucleotide sequence of a linear plasmid of Borrelia burgdorferi reveals similarities to those of circular plasmids of other prokaryotes.. J Bacteriol 178:6635–6639
    [Google Scholar]
  8. Baril C., Richaud C., Baranton G., SaintGirons I. 1989; Linear chromosome of Borrelia burgdorferi.. Res Microbiol 140:507–516
    [Google Scholar]
  9. Barthold S.W. 1993; Antigenic stability of Borrelia burgdorferi during chronic infections of immunocompetent mice.. Infect Immun 61:4955–4961
    [Google Scholar]
  10. Barthold S.W., de Souza M.S., Janotka J.L., Smith A.L., Persing D.H. 1993; Chronic Lyme borreliosis in the laboratory mouse.. Am J Pathol 143:959–972
    [Google Scholar]
  11. Burgdorfer W., Barbour A.G., Hayes S.F., Benach J.L., Grunwaldt E., Davis J.P. 1982; Lyme disease -a tick-borne spirochetosis?. Science 216:1317–1319
    [Google Scholar]
  12. Casjens S., Huang W.M. 1993; Linear chromosomal physical and genetic map of Borrelia burgdorferi, the Lyme disease agent.. Mol Microbiol 8:967–980
    [Google Scholar]
  13. Casjens S., van Vugt R., Tilly K., Rosa P.A., Stevenson B. 1997; Homology throughout the multiple 32-kilobase circular plasmids present in Lyme disease spirochetes.. J Bacteriol 179:217–227
    [Google Scholar]
  14. Craft J.E., Fischer D.K., Shimamoto G.T., Steere A.C. 1986; Antigens of Borrelia burgdorferi recognized during Lyme disease: appearance of a new immunoglobulin M response and expansion of the immunoglobulin G response late in the illness.. J Clin Invest 78:934–939
    [Google Scholar]
  15. Das S., Barthold S.W., Stocker Giles S., Montgomery R.R., Telford S.R., Fikrig E. 1997; Temporal pattern of Borrelia burgdorferi p21 expression in ticks and the mammalian host.. J Clin Invest 99:987–995
    [Google Scholar]
  16. Davidson B.E., MacDougail J., Saint Girons I. 1992; Physical map of the linear chromosome of the bacterium Borrelia burgdorferi212 a causative agent of Lyme disease, and localization of rRNA genes.. J Bacteriol 174:3766–3774
    [Google Scholar]
  17. Dunn J.J., Buchstein S.R., Butler L.-L., Fisenne S., Polin D.S., Lade B.N., Luft B.J. 1994; Complete nucleotide sequence of a circular plasmid from the Lyme disease spirochete, Borrelia burgdorferi.. J Bacteriol 176:2706–2717
    [Google Scholar]
  18. Dykhuizen D.E., Polin D.S., Dunn J., Wilske B., Preac-Mursic V., Dattwyler R.J., Luft B.J. 1993; Borrelia burgdorferi is clonal: implications for taxonomy and vaccine development.. Proc Natl Acad Sci USA 90:10163–10167
    [Google Scholar]
  19. Engstrom S.M., Shoop E., Johnson R.C. 1995; Immunoblot interpretation criteria for serodiagnosis of early Lyme disease.. J Clin Microbiol 33:419–427
    [Google Scholar]
  20. Ferdows M.S., Barbour A.G. 1989; Megabase-sized linear DNA in the bacterium Borrelia burgdorferi, the Lyme disease agent.. Proc Natl Acad Sci USA 86:5969–5973
    [Google Scholar]
  21. Fraser C.M., Casjens S., Huang W.M. & 35 other authors 1997; Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi.. Nature 390:580–586
    [Google Scholar]
  22. Fuchs R., Jauris S., Lottspeich F., Preac-Mursic V., Wilske B., Soutschek E. 1992; Molecular analysis and expression of a Borrelia burgdorferi gene encoding a 22kDa protein (pC) in Escherichia coli.. Mol Microbiol 6:503–509
    [Google Scholar]
  23. Gibbs C.P., Livey I., Dorner F. 1996; The role of re-combination in OspC variation in Lyme disease Borrelia.. Acta Dermatovenereol Alpina Pannonica Adriatica 5:179–183
    [Google Scholar]
  24. Higgins D.G., Bieasby A.J., Fuchs R. 1992; CLUSTAL V: improved software for multiple sequence alignment.. Comput Appl Biosci 8:189–191
    [Google Scholar]
  25. Jauris-Heipke S., Liegl G., Preac-Mursic V., Robler D., Schwab E., Soutschek E., Will G., Wilske B. 1995; Molecular analysis of genes encoding outer surface protein C (OspC) of Borrelia burgdorferi sensu lato: relationship to ospA genotype and evidence of lateral gene exchange of ospC.. J Clin Microbiol 33:1860–1866
    [Google Scholar]
  26. Lam T.T., Nguyen T.-P.K., Montgomery R.R., Kantor F.S., Fikrig E., Flavell R.A. 1994; Outer surface proteins E and F ofBorrelia burgdorferi, the agent of Lyme disease.. Infect Immun 62:290–298
    [Google Scholar]
  27. Livey I., Gibbs C.P., Schuster R., Dorner F. 1995; Evidence for lateral transfer and recombination in OspC variation in Lyme disease Borrelia.. Mol Microbiol 18:257–269
    [Google Scholar]
  28. Maniatis T., Fritsch E.F., Sambrook J. 1982 Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  29. Marconi R.T., Sung S.Y., Norton Hughes C.A., Carlyon J.A. 1996; Molecular and evolutionary analyses of a variable series of genes in Borrelia burgdorferi that are related to ospE and ospF, constitute a gene family, and share a common upstream homology box.. J Bacteriol 178:5615–5626
    [Google Scholar]
  30. Martin K.A., Davis M.A., Austin S. 1991; Fine-structure analysis of the PI plasmid partition site.. J Bacteriol 173:3630–3634
    [Google Scholar]
  31. Motallebi-Veshareh M., Rouch D.A., Thomas C.M. 1990; Afamily of ATPases involved in active partitioning of diverse bacterial plasmids.. Mol Microbiol 4:1455–1463
    [Google Scholar]
  32. Nguyen T.-P.K., Lam T.T., Barthold S.W., Telford S.R. III Flavell R.A., Fikrig E. 1994; Partial destruction of Borrelia burgdorferi within ticks that engorged on OspE- or OspF- immunized mice.. Infect Immun 62:2079–2084
    [Google Scholar]
  33. Novick R.P. 1987; Plasmid incompatibility.. Microbiol Rev 51:381–395
    [Google Scholar]
  34. Ogura T., Hiraga S. 1983; Partition mechanism of F plasmid: two plasmid gene-encoded products and a cis-acting region are involved in partition.. Cell 32:351–360
    [Google Scholar]
  35. Persing D.H., Mathiesen D., Podzorski D., Barthold S.W. 1994; Genetic stability of Borrelia burgdorferi recovered from chronically infected immunocompetent mice.. Infect Immun 62:3521–3527
    [Google Scholar]
  36. Porcella S.F., Popova T.G., Akins D.R., Li M., Radolf J.D., Norgard M.V. 1996; Borrelia burgdorferi supercoiled plasmids encode multi-copy tandem open reading frames and a lipoprotein gene family.. J Bacteriol 178:3293–3307
    [Google Scholar]
  37. Saint Girons I., Davidson B.E. 1992; Genome organization of Borrelia burgdorferi. . In Lyme Disease: Molecular and Immuno¬logic Approaches pp. 111–118 Ingilis J., Sitkowski J.W. Edited by Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  38. Simpson W.J., Garon C.F., Schwan T.G. 1990a; Analysis of supercoiled circular plasmids in infectious and non-infectious Borrelia burgdorferi.. Microb Pathog 8:109–118
    [Google Scholar]
  39. Simpson W.J., Garon C.F., Schwan T.G. 1990b; Borrelia burgdorferi contains repeated DNA sequences that are species specific and plasmid associated.. Infect Immun 58:847–853
    [Google Scholar]
  40. Stevenson B., Barthold S.W. 1994; Expression and sequence of outer surface protein C among North American isolates of Borrelia burgdorferi.. FEMS Microbiol Lett 124:367–372
    [Google Scholar]
  41. Stevenson B., Bockenstedt L.K., Barthold S.W. 1994; Expression and gene sequence of outer surface protein C of Borrelia burgdorferi reisolated from chronically infected mice.. Infect Immun 62:3568–3571
    [Google Scholar]
  42. Stevenson B., Schwan T.G., Rosa P.A. 1995; Temperature- related differential expression of antigens in the Lyme disease spirochete, Borrelia burgdorferi.. Infect Immun 63:4535–4539
    [Google Scholar]
  43. Stevenson B., Tilly K., Rosa P.A. 1996; A family of genes located on four separate 32-kilobase circular plasmids in Borrelia burgdorferi B31.. J Bacteriol 178:3508–3516
    [Google Scholar]
  44. Stevenson B., Casjens S., van Vugt R., Porcella S.F., Tilly K., Bono J.L., Rosa P. 1997; Characterization of cpl8, a naturally truncated member of the cp32 family of Borrelia burgdorferi plasmids.. J Bacteriol 179:4285–4291
    [Google Scholar]
  45. Stevenson B., Bono J.L., Schwan T.G., Rosa P. 1998; TheBorrelia burgdorferi Erp proteins are immunogenic in tick-bite- infected mammals and their synthesis is inducible in cultured bacteria.. Infect Immun (in press)
    [Google Scholar]
  46. Suk K., Das S., Sun W., Jwang B., Barthold S.W., Flavell R.A., Fikrig E. 1995; Borrelia burgdorferi genes selectively expressed in the infected host.. Proc Natl Acad Sci USA 92:4269–4273
    [Google Scholar]
  47. Theisen M., Frederiksen B., Lebech A.-M., Vuust J., Hansen K. 1993; Polymorphism in ospC gene of Borrelia burgdorferi and immunoreactivity of OspC protein: implications for taxonomy and for use of OspC protein as a diagnostic antigen.. J Clin Microbiol 31:2570–2576
    [Google Scholar]
  48. Theisen M., Borre M., Mathiesen M.J., Mikkelsen B., Lebech A.-M., Hansen K. 1995; Evolution of the Borrelia burgdorferi outer surface protein OspC.. J Bacteriol 177:3036–3044
    [Google Scholar]
  49. Tilly K., Casjens S., Stevenson B., Bono J.L., Hogan D., Rosa P. 1997; The Borrelia burgdorferi circular plasmid cp26: conservation of plasmid structure and targeted inactivation of the ospC gene.. Mol Microbiol 25:361–373
    [Google Scholar]
  50. Wallich R., Brenner C., Kramer M.D., Simon M.M. 1995; Molecular cloning and immunological characterization of a novel linear-plasmid-encoded gene, pG, of Borrelia burgdorferi expressed only in vivo.. Infect Immun 63:3327–3335
    [Google Scholar]
  51. Watanabe E., Wachi M., Yamasaki M., Nagai K. 1992; ATPase activity of SopA, a protein essential for active partitioning of F plasmid.. Mol Gen Genet 234:346–352
    [Google Scholar]
  52. Wilske B., Preac-Mursic V., Schierz G., Busch K.V. 1986; Immunochemical and immunological analysis of European Borrelia burgdorferi strains.. Zentralbl Bakteriol Hyg A 263:92–102
    [Google Scholar]
  53. Xu Y., Johnson R.C. 1995; Analysis and comparison of plasmid profiles of Borrelia burgdorferi sensu lato strains.. J Clin Microbiol 33:2679–2685
    [Google Scholar]
  54. Zhang J.-R., Hardham J.M., Barbour A.G., Norris S.J. 1997; Antigenic variation in Lyme disease borreliae by promiscuous recombination of VMP-like sequence cassettes.. Cell 89:1–20
    [Google Scholar]
  55. Zückert W.R., Meyer J. 1996; Circular and linear plasmids ofLyme disease spirochetes have extensive homology: charac-terization of a repeated DNA element.. J Bacteriol 178:2287–2298
    [Google Scholar]
  56. Zückert W.R., Filipuzzi-Jenny E., Meister-Turner J., Stålmmar-Carlemalm M., Meyer J. 1994; Repeated DNA sequences on circular and linear plasmids of Borrelia burgdorferi sensu lato.. In Lyme Borreliosis pp. 253–260 Axford J.S., Rees D. H. E. Edited by New York: Plenum Press;
    [Google Scholar]
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