1887

Microbiology Society logo

Volume 144, Issue 7

Starvation recovery of Staphylococcus aureus 8325-4 Free

Abstract

Nutrient limitation of Staphylococcus aureus induces a starvation-survival state which enables it to survive until sufficient nutrients become available to support growth. The response of starved S. aureus cells to nutritional upshift was analysed to characterize the recovery mechanism which results in the resumption of rapid growth. S. aureus 8325-4 starved for 7 d in a chemically defined medium limited for glucose was able to resume growth upon the addition of complex medium (brain heart infusion broth) or a mixture of amino acids and glucose. The addition of either glucose or amino acids alone did not lead to recovery of cells. Prior to the first cell division event, a lag period of about 120--150 min was observed, the duration of which was independent of the length of starvation survival. During this lag period, RNA synthesis increased immediately upon the addition of nutrients whilst protein synthesis was delayed by approximately 5 min. Cells rapidly enlarged within 30 min of recovery, and initiation of chromosome replication could be detected after 90 min. Changes in the profile of proteins expressed during the recovery period revealed that several starvation-specific proteins were down-regulated within 30 min, whilst other proteins were common to both starvation and recovery. Two proteins were identified which were only transiently expressed during the first 60 min of recovery. Protein synthesis could be detected during recovery even if the cells had been treated with the RNA synthesis inhibitor rifampicin for 30 min prior to the addition of recovery nutrients, demonstrating that several proteins are translated from long-lived mRNA transcripts present in starved cells.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): long-lived mRNA , recovery , recovery-specific proteins , Staphylococcus aureus and starvation
© Society for General Microbiology 1998
Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-144-7-1755
1998-07-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/144/7/mic-144-7-1755.html?itemId=/content/journal/micro/10.1099/00221287-144-7-1755&mimeType=html&fmt=ahah

References

  1. Ākerlund T., Nordström K., Bernander R. 1995; Analysis of cell size and DNA content in exponentiallygrowing and stationary phase batch cultures of Escherichia coli. . J Bacteriol 177:6791–6797
     [Google Scholar]
  2. Albertson N. H., Nystrom T., Kjelleberg S. 1990a; Functional mRNA half-lives in the marine Vibrio sp. S14 during starvation and recovery.. J Gen Microbiol 136:2195–2199
     [Google Scholar]
  3. Albertson N. H., Nystrom T., Kjelleberg S. 1990b; Macromolecular synthesis during recovery of the marineVibrio sp. S14 from starvation.. J Gen Microbiol 136:2201–2207
     [Google Scholar]
  4. Aldea M., Hernandezchico C., Delacampa A.G., Kushner S.R., Vicente M. 1988; Identification, cloning and expression of bolA,an ftsZ dependent morphogene of Escherichia coli. . J Bacteriol 170:5169–5176
     [Google Scholar]
  5. Ball C.A., Osuna R., Ferguson K.C., Johnson R.C. 1992; Dramatic changes in Fis levels upon nutrient upshift in Escherichia coli. . J Bacteriol 174:8043–8056
     [Google Scholar]
  6. Diaper J.P., Edwards C. 1994; Survival of Staphylococcus aureus in lakewater monitored by flow-cytometry.. Microbiology 140:35–42
     [Google Scholar]
  7. Donachie W.D., Robinson A.C. 1987; Cell division: parameter values and the process. In Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology . 2: pp. 1578–1593 Neidhardt F.C. Edited by others Washington, DC:: American Society for Microbiology.;
     [Google Scholar]
  8. Fiil N., Friesen J.D. 1968; Isolation of relaxed mutants of Escherichia coli. . J Bacteriol 175:53–63
     [Google Scholar]
  9. Flärdh K., Cohen P.S., Kjelleberg S. 1992; Ribosomes exist in large excess over the apparent demand for protein synthesis during carbon starvation in marine Vibrio sp. Strain CCUG 15956.. J Bacteriol 174:6780–6788
     [Google Scholar]
  10. Givskov M., Eberl L., Moller S., Poulsen L. K., Molin S. 1994a; Responses to nutrient starvation in Pseudomonas putida KT2442: analysis of general cross protection, cell shape and macromolecular content.. J Bacteriol 176:7–14
     [Google Scholar]
  11. Givskov M., Eberl L., Molin S. 1994b; Responses to nutrient starvation in Pseudomonas putida KT2442: two-dimensional electrophoretic analysis of starvation- and stress-induced proteins.. J Bacteriol 176:4816–4824
     [Google Scholar]
  12. Hengge-Aronis R. 1996; Regulation of gene expression during entry into stationary phase. In. Escherichia coli and Salmonella: Cellular and Molecular Biology pp. 1497–1512 Neidhardt F.C. Edited by others Washington, DC:: American Society for Microbiology.;
     [Google Scholar]
  13. Higgins C.F., Causton H.C., Dance G.S.C., Mudd E.A. 1993; The role of 3′-end in mRNA stability and decay.. In Control of Messenger RNA Stability pp. 13–30 Belasco J., Brawerman G. Edited by San Diego, CA:: Academic Press.;
     [Google Scholar]
  14. Hussain M., Hastings J., White P.J. 1991; A chemically defined medium for slime production by coagulase-negative staphylococci.. J Med Microbiol 34:143–147
     [Google Scholar]
  15. Kaprelyants A.S., Mukamolova G.V., Kell D.B. 1994; Estimation of dormant Micrococcus luteus cells by penicillin lysis and by resuscitation in cell-free spent culture medium at high dilution.. FEMS Microbiol Lett 115:347–352
     [Google Scholar]
  16. Kjelleberg S., Albertson N., Flärdh K., Holmquist L., Jouperjaan A., Marouga R., Ostling J., Svenblad B., Weichart D. 1993; How do non-differentiating bacteria adapt to starvation ?. Antonie Leeuwenhoek 63:331–341
     [Google Scholar]
  17. Kloos W.E., Bannerman T.L. 1994; Update on clinical significance of coagulase-negative staphylococci.. Clin Microbiol Rev 7:117–140
     [Google Scholar]
  18. Kolter R., Siegele D.A., Tormo A. 1993; The stationary-phase of the bacterial life-cycle.. Annu Rev Microbiol 47:855–874
     [Google Scholar]
  19. Marouga R., Kjelleberg S. 1996; Synthesis of immediate upshift (Iup) proteins during recovery of marine Vibrio sp. strain S14 subjected to long-term carbon starvation.. J Bacteriol 178:817–822
     [Google Scholar]
  20. Mosteller R.D. 1978; Evidence that glucose starvation-sensitive mutants are altered in relB locus.. J Bacteriol 133:1034–1037
     [Google Scholar]
  21. Mukamolova G. V., Yanopolskaya N. D., Votyakova T. V., Popov V. I., Kaprelyants A. S., Kell D. B. 1995; Biochemical changes accompanying the long-term starvation of Micrococcus luteus cells in spent growth-medium.. Arch Microbiol 163:373–379
     [Google Scholar]
  22. Mulvey M.R., Switala J., Borys A., Loewen P.C. 1990; Regulation of transcription of katE and katF in Escherichia coli. . J Bacteriol 172:6713–6720
     [Google Scholar]
  23. Novick R.P. 1967; Properties of a high-frequency transducing phage in Staphylococcus aureus. . Virology 33:155–156
     [Google Scholar]
  24. Nystrom T., Olsson R.M., Kjelleberg S. 1991; Survival, stress resistance, andalteration in protein expression in the marine Vibrio sp. strain S14 during starvation for different individua nutrients.. Appl Environ Microbiol 58:55–65
     [Google Scholar]
  25. Oliver J.D. 1993; Formation of viable but non-culturable cells. In Starvation in Bacteria, . pp. 239–268 Kjelleberg S. Edited by New York:: Plenum.;
     [Google Scholar]
  26. Siegele D.A., Guynn L.J. 1996; Escherichia coli proteins synthesized during recovery from starvation.. J Bacteriol 178:6352–6356
     [Google Scholar]
  27. Siegele D.A., Kolter R. 1993; Isolation and characterisation of an Escherichia coli mutant defective in resuming growth after starvation.. Genes Dev 7:2629–2640
     [Google Scholar]
  28. Spector M.P., Cubitt C.L. 1992; Starvation-inducible loci of Salmonella typhimurium - regulation and roles in starvation- survival.. Mol Microbiol 6:1467–1476
     [Google Scholar]
  29. Spector M.P., Aliabadi Z., Gonzalez T., Foster J.W. 1986; Global control in Salmonella typhimurium - two-dimensional electrophoretic analysis of starvation-inducible, anaerobiosis- inducible, and heat shock-inducible proteins.. J Bacteriol 168:420–424
     [Google Scholar]
  30. Uhde C., Schmidt R., Jording D., Selbitschka W., Pühler A. 1997; Stationary-phase mutants of Sinorhizobium meliloti are impaired in stationary-phase survival or in recovery to logarithmic growth.. J Bacteriol 179:6432–6440
     [Google Scholar]
  31. Votyakova T.V., Kaprelyants A.S., Kell D.B. 1994; Influence of viable cells on the resuscitation of dormant cells in Micrococcus luteus cultures held in an extended stationary phase: the population effect.. Appl Environ Microbiol 60:3284–3291
     [Google Scholar]
  32. Watson S.P., Clements M.O., Foster S.J. 1998; Characterisation of the starvation-survival response of Staphylococcus aureus. . J Bacteriol 180:1750–1758
     [Google Scholar]
  33. Xu H.S., Roberts N., Singleton F.L., Attwell R.W., Grimes D.J., Colwell R.R. 1982; Survival and viability of non- culturable Escherichia coli and Vibrio cholerae in the estuarine and marine environment.. Microb Ecol 8:313–323
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-144-7-1755
Loading
Starvation recovery of Staphylococcus aureus 8325-4
Microbiology 144, 1755 (1998); https://doi.org/10.1099/00221287-144-7-1755
/content/journal/micro/10.1099/00221287-144-7-1755
/content/journal/micro/10.1099/00221287-144-7-1755
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error