1887

Abstract

Fatty acid composition was determined during several stages of sexual development in Neurospora crassa. Triacylglycerol was the predominant acyl lipid in cultures undergoing sexual development. The absolute amounts of triacylglycerol in fertilized cultures varied over time, in contrast to control (unfertilized or mock-fertilized) cultures, in which the amount of triacylglycerol decreased linearly with age. In cultures competent to undergo sexual development, -linoleate was the predominant fatty acid, ranging from 53 to 65% of the total fatty acid mass. -Linolenate was 3% or less of the total fatty acid, in marked contrast to the much higher levels (10--35%) typically reported for vegetative cultures. In fertilized cultures, a slightly higher mass ratio of oleate was also observed. This difference was due to the developing asci: in developing asci and mature ascospores, oleate replaced α-linoleate as the predominant fatty acid (45 to 50% of the total). In germinating ascospores, the fatty acid composition approached that of vegetative cultures 6 h after inducing germination by heat activation. These results show that the fatty acid composition of sexual tissues of Neurospora differs substantially from the composition of asexual tissues, and that extensive changes in fatty acid composition correlate with several events in the sexual stage of development.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-144-7-1713
1998-07-01
2021-09-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/144/7/mic-144-7-1713.html?itemId=/content/journal/micro/10.1099/00221287-144-7-1713&mimeType=html&fmt=ahah

References

  1. Aaronson L.R., Johnston A.M., Martin C.E. 1982; The effects of temperature acclimation on membrane sterols and phospholipids of Neurospora crassa. . Biochim Biophys Acta 713:456–462
    [Google Scholar]
  2. Baker N., Lynen F. 1971; Factors involved in fatty acyl CoA desaturation by fungal microsomes. The relative roles of acyl CoA and phospholipids as substrates.. Eur J Biochem 19:200–210
    [Google Scholar]
  3. Ballou L.R., Bianchi D.E. 1978; Lipids associated with microconidial differentiation in a mutant of Neurospora crmsa. . Curr Microbiol 1:111–115
    [Google Scholar]
  4. Bianchi D.E., Turian G. 1967; Lipid content of conidia of Neurospora crassa. . Nature 214:1344–1345
    [Google Scholar]
  5. Brooks J., Shaw G. 1978; Sporopollenin : a review of its chemistry, palaeochemistry and geochemistry.. Grana 17:91–97
    [Google Scholar]
  6. Cote G.G., Brody S. 1987; Circadian rhythms in Neurospora crassa : a clock mutant, prd-1, is altered in membrane fatty acid composition.. Biochim Biophys Acta 904:131–139
    [Google Scholar]
  7. Davis R.H., deSerres F.J. 1970; Genetic and microbiological research techniques for Neurospora crassa. . Methods Enzymol 17A:79–143
    [Google Scholar]
  8. Dyer P.S., Ingram D.S., Johnstone K. 1993; Evidence for the involvement of linoleic acid and other endogenous lipid factors in perithecial development of Nectria haematococca mating population VI. . Mycol Res 97:485–496
    [Google Scholar]
  9. Goodrich-Tanrikulu M., Stafford A.E., Lin J.-T., Makapugay M.I., Fuller G., McKeon T.A. 1994; Fatty acid biosynthesis in novel ufa mutants of Neurospora crassa. . Microbiology 140:2683–2690
    [Google Scholar]
  10. Goodrich-Tanrikulu M., Lin J.-T., Stafford A.E., Makapugay M.I., McKeon T.A., Fuller G. 1995; Novel Neurospora crassa mutants with altered synthesis of polyunsaturated fatty acids. . Microbiology 141:2307–2314
    [Google Scholar]
  11. Islam M.S. 1981; Sex pheromones in Neurospora crassa. . In Sexual Interactions in Eukaryotic Microbes pp. 131–154
    [Google Scholar]
  12. O̒Day D.H., Horgen P.A. Edited by New York: Academic Press;
  13. Kushwaha S.C, Kates M., Kramer J.K.G., Subden R.E. 1976; Lipid composition of Neurospora crassa. . Lipids 11:778–780
    [Google Scholar]
  14. Lindegren C.C, Beanfield V., Barber R. 1939; Increasing the fertility of Neurospora by selective inbreeding. . Bot Gaz 100:592–599
    [Google Scholar]
  15. Lingappa B.T., Sussman A.S. 1959; Endogenous substrates of dormant, activated and germinating ascospores of Neurospora tetrasperma. . Plant Physiol 34:466–472
    [Google Scholar]
  16. Lyudnikova T.A., Chernysheva E.K., Bezzubov A.A., Kritskii M.S. 1990; Interrelationship of desaturation of fatty acids of phospholipids and the synthesis of carotenoids during adaptation of Neurospora crassa cells to temperature and light influence. . Biochemistry (English translation of Biokhimiya) 55:1647–1653
    [Google Scholar]
  17. Marshall M.R., Hindal D.F., MacDonald W.L. 1982; Production of perithecia in culture by Ceratocystis ulmi. . Mycologia 74:376–381
    [Google Scholar]
  18. Martin C.E., Siegel D., Aaronson L.R. 1981; Effects of temperature acclimation on Neurospora phospholipids: fatty acid desaturation appears to be a key element in modifying phospholipid fluid properties. . Biochim Biophys Acta 665:399–407
    [Google Scholar]
  19. Mazur P., Meyers H.V., Nakanishi K., El-Zayat A.A.E., Champe S.P. 1990; Structural elucidation of sporogenic fatty acid metabolites from Aspergillus nidulans. . Tetrahedron Lett 31:3837–3840
    [Google Scholar]
  20. Mazur P., Nakanishi K., El-Zayat A.A.E., Champe S.P. 1991; Structure and synthesis of sporogenic psi factors from Aspergillus nidulans. . J Chem Soc D Chem Commun 20:1486–1487
    [Google Scholar]
  21. Nelson M.A. 1996; Mating systems in ascomycetes: a romp in the sac. . Trends Genet 12:69–74
    [Google Scholar]
  22. Nukina M., Sassa T., Ikeda M., Takahasi K., Toyota S. 1981; Linoleic acid enhances perithecial production in Neurospora crassa. . Agric Biol Chem 45:2371–2373
    [Google Scholar]
  23. Perkins D.D. 1986; Hints and precautions for the care, feeding and breeding of Neurospora. . Fungal Genet Newsl 33:35–41
    [Google Scholar]
  24. Perkins D.D., Pollard V.C. 1987; Newly mapped chromosomal loci of Neurospora crassa. . Fungal Genet Newsl 34:52–53
    [Google Scholar]
  25. Raju N.B. 1992; Genetic control of the sexual cycle in Neurospora. . Mycol Res 96:241–262
    [Google Scholar]
  26. Roeder P.E., Sargent M.L., Brody S. 1982; Circadian rhythms in Neurospora crassa: oscillations in fatty acids. . Biochemistry 21:4909–4916
    [Google Scholar]
  27. Saupe S., Descamps C., Turcq B., Bégueret J. 1994; Inactivation of the Podospora anserina vegetative incompatibility locus het-c, whose product resembles a glycolipid transfer protein, drastically impairs ascospore production. . Proc Natl Acad Sci USA 915927–5931
    [Google Scholar]
  28. Siddiq A.A., Ingram D.S., Johnstone K., Friend J., Ashby A.M. 1989; The control of asexual and sexual development by morphogens in fungal pathogens. . Asp Appl Biol 23:417–426
    [Google Scholar]
  29. Vokt J.P., Brody S. 1985; The kinetics of changes in the fatty acid composition of Neurospora crassa lipids after a temperature increase. . Biochim Biophys Acta 835:176–182
    [Google Scholar]
  30. Westergaard M., Mitchell H.K. 1947; Neurospora V. A synthetic medium favoring sexual reproduction. . Am J Bot 34:573–577
    [Google Scholar]
  31. Zaki A.I., Zentmyer G.A., Sims J.J., Keen N.T. 1983; Stimulation of sexual reproduction in the A2 mating type of Phytophthora cinnamomi by oleic acid and lipids from avocado roots. . Phytopathology 73:199–203
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-144-7-1713
Loading
/content/journal/micro/10.1099/00221287-144-7-1713
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error