1887

Abstract

Summary: DNA gyrase, an essential type II topoisomerase, mediates negative supercoiling of the bacterial chromosome, thereby affecting the processes of DNA replication, transcription, recombination and repair. The gene from the Gram-negative soil bacterium was sequenced. The sequence predicts a protein of 815 amino acid residues displaying significant homology to all known GyrB proteins. A 6-His-GyrB fusion protein was overexpressed in and purified to near homogeneity using affinity chromatography on Ni-nitrilotriacetic acid-agarose and novobiocin-Sepharose columns. The fusion protein bound novobiocin and cross-reacted with anti- GyrB antibodies, indicating structural and functional similarities to the DNA GyrB. The gene was mapped to the region of the origin of replication (

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/content/journal/micro/10.1099/00221287-144-6-1641
1998-06-01
2019-10-13
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-144-6-1641
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