1887

Abstract

A group of sea-ice-derived psychrophilic bacterial strains possessing the unusual ability to synthesize the polyunsaturated fatty acids eicosapentaenoic acid (20:5.3) and arachidonic acid (20:4.6) belong to the Family ( phylum), according to 16S rRNA sequence analysis. Surprisingly, the isolates were also found to cluster closely to the moderately halophilic and psychrotrophic species [] gondwanense (sequence similarity 97-8-98-1 %). The whole-cell fatty acid profiles of this group and [] were very similar and distinct from other related flavobacteria. The sea ice strains and [] differed substantially in terms of ecophysiology, possibly representing divergent adaptations to sympagic and planktonic marine habitats, respectively. Evidence based on phylogeny and fatty acid profiles supports the conclusion that the taxa are close relatives distinct from other bacterial groups. It is thus proposed that the sea ice strains represent a novel taxon designated gen. nov., sp., nov. (type strain ACAM 623) while [] becomes gen. nov., comb. nov.

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1998-06-01
2021-08-05
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References

  1. Bernardet, J.-F., Segers, P., Vancanneyt, M., Berthe, F., Kersters, K., Vandamme, P. (1996) Cutting a gordian knot: emended classification and description of the genus Flavobacterium, emended description of the Family Flavobacteriaceae and proposal of Flavobacterium hydatis nom.nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978).. Int J Syst Bacterial. 46,128–148
    [Google Scholar]
  2. Bowman, J. P.,, Brown, M. V., Nichols, D. S. (1997a); Biodiversity and ecophysiology of bacteria associated with Antarctic sea ice.. Antarct Sci 9,:(2)134–142 [View Article]
    [Google Scholar]
  3. Bowman, J. P., McCammon, S. A., Brown, M. V., Nichols, D. S., McMeekin, T. A. (1997b); Diversity and association of psychro- philic bacteria in Antarctic sea ice.. Appl Environ Microbiol 63,:(8)3068–3078 [View Article]
    [Google Scholar]
  4. Bowman, J. P., McCammon, S. A., Brown, J. L., Nichols, P. D., McMeekin, T. A. (1997c) Psychroserpens burtonensis gen. nov., sp. nov., and Gelidibacter algens gen. nov., sp. nov., psychrophilic bacteria from Antarctic lacustrine and sea ice habitats. . Int J Syst Microbiol 47,670–677
    [Google Scholar]
  5. Christian, J. R., Karl, D. M. (1995); Bacterial ectoenzymes in marine waters: activity ratios and temperature responses in three oceanographic provinces.. Limnology and Oceanography, 40,:(6)1042–1049 [View Article]
    [Google Scholar]
  6. Delille, D. (1996); Biodiversity and function of bacteria in the Southern Ocean.. Biodivers and Conserv 5,:(11)1505–1523 [View Article]
    [Google Scholar]
  7. DeLong, E. F., Franks, D. G., Yayanos, A. A. (1997); Evolutionary relationships of cultivated psychrophilic and barophilic deep-sea bacteria.. Appl Environ Microbiol 63,:(5)2105–2108 [View Article]
    [Google Scholar]
  8. Dobson, S. J., Colwell, R. R., McMeekin, T. A., Franzmann, P. D. (1993); Direct sequencing of the polymerase chain reaction- amplified 16S rRNA gene of Flavobacterium gondwanense sp. nov. and Flavobacterium salegens sp. nov., two new species from a hypersaline Antarctic lake.. Int Znt J Syst Bacteriol 43,:(1)77–83 [View Article]
    [Google Scholar]
  9. Felsenstein, J. (1993) PHYLIP (phylogeny inference package), version 3.57c. Seattle:: University of Washington.;
    [Google Scholar]
  10. Franzmann, P. D., Deprez, P. P., McGuire, A. J., McMeekin, T. A., Burton, H. R. (1990); The heterotrophic bacterial microbiota of Burton Lake, Antarctica. Polar Biol 10,:261–264
    [Google Scholar]
  11. Gosink, J. J., Staley, J. T. (1995); Biodiversity of gas vacuolate bacteria from Antarctic sea ice and water.. Appl Environ Microbiol 61,:(9)3486–3489 [View Article]
    [Google Scholar]
  12. Gosink, J. J., Woese, C. R., Staley, J. T. (1998) Polaribacter gen. nov., with three new species, filamentus sp. nov., gas vacuolate polar marine bacteria of the Cytophaga-Flavobacterium-Bacteroides group and reclassification of' Flectobacillus glomeratus ’ as Polaribacter glomeratus comb. nov.. Int J Syst Bacteriol. 48,223–235
    [Google Scholar]
  13. Helmke, E., Weyland, H. (1995); Bacteria in sea ice and underlying water of the eastern Weddell Sea in midwinter.. Mar Ecol Prog Ser 117,269–287
    [Google Scholar]
  14. Huss, V. A. R., Festl, H., Schleifer, K.-H. (1983); Studies on the spectrophotometric determination of DNA hybridization from renaturation rates.. Syst Appl Microbiol 4,:(2)184–192 [View Article]
    [Google Scholar]
  15. Irgens, R., Gosink, J. J., Staley, J. T. (1996); Polaromonas vacuolata gen. nov., sp. nov., a psychrophilic, marine, gas vacuolate bacterium from Antarctica.. Int J Syst Bacteriol 46,:(3)822–826 [View Article]
    [Google Scholar]
  16. Iwanami, H., Yamaguchi, T., Takeuchi, M. (1995); Fatty acid metabolism in bacteria that produce eicosapentaenoic acid isolated from sea urchin Strongylocentrotus nudus.. Nippon Suisan Gakkaisbi 61,:(2)205–210 [View Article]
    [Google Scholar]
  17. James, S. R., Burton, H. R., McMeekin, T. A., Mancuso, C. A. (1994); Seasonal abundance of Fialomonas meridiana, Halomonas subglaciescola, Flavobacterium gondwanense and Flavobacterium salegens.. Antarct Sci 6,:(3)325–332 [View Article]
    [Google Scholar]
  18. Johns, R. B., Perry, G. J. (1977); Lipids of the marine bacterium Flexibacter polymorpbus.. Arch of Microbiol 114,:(3)267–271 [View Article]
    [Google Scholar]
  19. Kaneda, T. (1991); Iso- and anteiso-fatty acids in bacteria: biosynthesis, function and taxonomic significance.. Microbiol Rev 55,:(2)288–302 [View Article]
    [Google Scholar]
  20. Malinsky-Rushansky, N. Z., Legrand, C. (1996); Excretion of dissolved organic carbon by phytoplankton of different sizes and subsequent bacterial uptake.. Mar Ecol Prog Ser 132,:249–255 [View Article]
    [Google Scholar]
  21. Marmur, J., Doty, P. (1962); Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature.. J Mol Biol 5,:(1)109–118 [View Article]
    [Google Scholar]
  22. Nichols, D. S., Brown, J. L., Nichols, P. D., McMeekin, T. A. (1997); Production of eicosapentaenoic acid and arachidonic acids by an Antarctic bacterium: response to growth temperature.. FEMS Microbiol Lett 152:(2)349–354 [View Article]
    [Google Scholar]
  23. Nichols, P. D., Guckert, J. B., White, D. C. (1986); Determination of monounsaturated fatty acid double-bond position and geometry for microbial monocultures and complex consortia by capillary GC-MS of their dimethyldisulphide adducts.. J Microbiol Methods 5,:(1)49–55 [View Article]
    [Google Scholar]
  24. Palmisano, A. C., Garrison, D. L. (1993) Microorganisms in Antarctic sea ice.. Edited by Friedmann, E. I. Antarctic Microbiology. New York:: Wiley-Liss,;
    [Google Scholar]
  25. Priddle, J., Leakey, R. J. G., Archer, S. D., Murphy, E. J. (1996); Eukaryotic microbiota in the surface waters and sea ice of the Southern Ocean : aspects of physiology, ecology and biodiversity in a ′two-phase′ ecosystem. . Biodivers Conserv 5,1473–1504
    [Google Scholar]
  26. Ratkowsky, D. A., Lowry, R. K., McMeekin, T. A., Stokes, A. N. , Chandler, R. E. (1983); Eukaryotic microbiota in the surface waters and sea ice of the Southern Ocean: aspects of physiology, ecology and biodiversity in a ‘two-phase’ ecosystem, Model for bacterial growth throughout the entire biokinetic range.. J Bacteriol 154,:1222–1226
    [Google Scholar]
  27. Sly, L. I., Blackall, L. L., Kraat, P. C., Tian-Shen, T., Sangkhobol, V. (1986); The use of second derivative plots for the determination of mol % guanine plus cytosine of DNA by the thermal denaturation method.. J Microbiol Methods 5,:(3-4)139–156 [View Article]
    [Google Scholar]
  28. Smibert, R. M., Krieg, N. R. (1994) Phenotypic characterization.. Edited by Gerhardt, P., Murray, R. G., Wood, W. A., Krieg, N. R. Methods for General and Molecular Microbiology. Washington, DC:: American Society for Microbiology,;611–654
    [Google Scholar]
  29. Sprecher, H., Luthria, D. L., Mohammed, B. S., Baykousheva, S. P. (1995); Re-evaluation of the pathways for the biosynthesis of polyunsaturated fatty acids.. J Lipid Res 36,:(12)2471–2477 [View Article]
    [Google Scholar]
  30. Sullivan, C. W., Palmisano, A. C. (1984); Sea ice microbial communities: distribution, abundance and diversity of ice bacteria in McMurdo Sound,. Antarctica in 1980 47,:788–795
    [Google Scholar]
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