1887

Abstract

Incubation of NCPF 2140 in growth medium containing 1 % chitin as sole carbon source led to induction of specific extracellular chitinolytic activity of 1.5 μmol GlcNAc released min (mg protein)-1. The effect was repressed by the inclusion of GlcNAc in the medium, indicating regulation by a negative feedback mechanism. Extracellular chitinase activity was inhibited by allosamidin (IC 0.12 μM). Multiple chitinolytic enzymes were detected on zymograms of extracellular preparations; levels of individual enzymes induced were dependent upon whether cells were incubated with purified colloidal chitin or a crude preparation of crystalline chitin. A major, inducible, 45 kDa chitinase was purified using ammonium sulphate precipitation, chitin affinity chromatography and a novel procedure involving the electroelution of the enzyme from a substrate gel containing glycol chitin. The enzyme is a glycoprotein with endochitinase activity.

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1998-06-01
2021-07-30
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References

  1. Adams, D. J., Escott, G. M. (1997) Chitinases of fungal pathogens and human blood cells.. Edited by Suzuki M., Suzuki S. Fungal Cells in Biodefense Mechanism. Tokyo:: Saikon Publishing,;233–238
    [Google Scholar]
  2. Barnes, A. J., Denning, D. W. (1993); Aspergilli - significance as pathogens.. Reviews in Medical Microbiology, 4,:176–180
    [Google Scholar]
  3. Bradford, M. M. (1976); A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein—dye binding.. Analytical Biochemistry, 72,:248–254
    [Google Scholar]
  4. Cabib, E. (1987); The synthesis and degradation of chitin.. Adv Enzymol, 59,:59–101
    [Google Scholar]
  5. Campbell, C. K. (1994) Forms of aspergillosis.. Edited by Powell, K. A., Renwick, A., Peberdy, J. F. The Genus Aspergillus. New York:: Plenum,;313–317
    [Google Scholar]
  6. Cohen, J. (1991) Clinical manifestations and management of aspergillosis in the compromised patient.. Edited by Warnock, D. W., Richardson, M. D. Fungal Infection in the Compromised Patient. Colchester:: Wiley,;117–152
    [Google Scholar]
  7. Dewald, B., Dulaney, J. T., Touster O. (1974); Solubilisation and polyacrylamide gel electrophoresis of membrane enzymes with detergents.. Methods in Enzymology, Vol 46: Research on Nitrification and Related Processes, Pt B, 32,:82–91
    [Google Scholar]
  8. Dickinson, K., Keer, V., Hitchcock, C. A., Adams, D. J. (1989); Chitinase activity from Candida albicans and its inhibition by allosamidin.. Journal of General Microbiology, 135,:1417–1421
    [Google Scholar]
  9. Escott, G. M., Adams, D. J. (1995); Chitinase activity in human serum and leukocytes.. Infection and Immunity, 63,:4770–4773
    [Google Scholar]
  10. Findlay, J. B. C., Pappin, D. J. C., Keen, J. N. (1989) Automated solid-phase microsequencing.. Edited by Findlay J. B. C., Geisow. C M. J. Protein Sequencing. A Practical Approach. Oxford:: IRL Press,;69–84
    [Google Scholar]
  11. Flach, J., Pilet, P. E., Jolles, P. (1992); What’s new in chitinase research?. Experientia, 48,:701–716
    [Google Scholar]
  12. Gooday, G. W. (1990); The ecology of chitin degradation.. Advances in Microbial Ecology, 11,:387M30
    [Google Scholar]
  13. Gooday, G. W. (1996) Aggressive and defensive roles for chitinases.. Edited by Muzzarelli, R. A. A. Chitin Enzymology. vol. 2: Grottammare:: Atec Edizione,;125–134
    [Google Scholar]
  14. Gooday, G. W., Zhu, W.-Y., O'Donnell, R. W. (1992); What are the roles of chitinases in the growing fungus?. Fems Microbiology Letters, 100,:387–392
    [Google Scholar]
  15. Graham, L. S., Sticklen, M. B. (1994); Plant chitinases.. Can J Bot 72:1057–1083
    [Google Scholar]
  16. Hearn, V. M., Escott, G. M., Evans, E. G. V. , Adams D. J. (1996); Chitinases of the cell surface and wall of Aspergillus fumigatus.. Edited by Muzzarelli, R. A. A. Chitin Enzymology. vol. 2: Grottammare:: Atec Edizione,;261–272
    [Google Scholar]
  17. Hearn, V. M., Escott, G. M., Evans, E. G. V., Adams, D. J. (1997) Edited by Suzuki M., Suzuki S. Intracellular and wall-associated chitinases of Aspergillus fumigatus.. Fungal Cells in Biodefense Mechanism. Tokyo:: Saikon Publishing,;247–252
    [Google Scholar]
  18. Hitchcock, C. A., Dickinson, K., Brown, S. B., Evans, E. G. V., Adams, D. J. (1989); Purification and properties of cytochrome P450-dependent 14a-sterol demethylase from Candida albicans.. Biochemical Journal, 263,:573–579
    [Google Scholar]
  19. Lewis, C. W., Anderson, J. G., Smith, J. E. (1994) Health -related aspects of the genus Aspergillus.. Edited by Smith, J. E. Aspergillus. New York:: Plenum,;219–261
    [Google Scholar]
  20. Mellor, K. J., Nicholas, R. O., Adams, D. J. (1994); Purification and characterisation of chitinase from Candida albicans.. Fems Microbiology Letters, 119,:111–118
    [Google Scholar]
  21. Molano, J., Duran, A., Cabib, E. (1977); A rapid and sensitive assay for chitinase using tritiated chitin.. Analytical Biochemistry, 83,:648–656
    [Google Scholar]
  22. Monreal, J., Reese, E. T. (1969); The chitinase of Serratia marcescens.. Canadian Journal of Microbiology, 15,:689–696
    [Google Scholar]
  23. Overdijk, B., Van Steijn, G. J. (1994); Human serum contains a chitinase: identification of an enzyme, formerly described as 4- methylumbelliferyl-tetra-N-acetylchitotetraoside hydrolase (MU-TACT hydrolase).. Glycobiology, 4,:797–803
    [Google Scholar]
  24. Pan, S. Q., Ye, X. S., Kuc, J. (1991); A technique for detection of chitinase, /?-l,3-glucanase and protein patterns after a single separation using polyacrylamide gel electrophoresis or isoelectric focusing.. Phytopathology, 81,:970–974
    [Google Scholar]
  25. Renkema, G. H., Boot, R. G., Muijsers, A. O., Donker-Koopman, W. E., Aerts, J. M. F. G. (1995); Purification and characterization of human chitotriosidase, a novel member of the chitinase family of proteins.. Journal of Biological Chemistry, 270,:2198–2202
    [Google Scholar]
  26. Sahai, A. S., Manocha, M. S. (1993); Chitinases of fungi and plants: their involvement in morphogenesis and host-parasite interaction.. Fems Microbiology Reviews, 11,:317–338
    [Google Scholar]
  27. St Leger, R. J., Staples, R. C., Roberts, D. W. (1993); Entomo- pathogenic isolates of Metarhizium anisopliae, Beauveria bassiana and Aspergillus flavus produce multiple extracellular chitinase isoenzymes.. Journal of Invertebrate Pathology, 61,:81–84
    [Google Scholar]
  28. Studer, M., Fliick, K., Zimmermanm, W. (1992); Production of chitinases by Aphanocladium album grown on crystalline and colloidal chitin.. Fems Microbiology Letters, 99,:213–216
    [Google Scholar]
  29. Tronsmo, A., Harman, G. E. (1993); Detection and quantification of N-acetyl-/?-D-glucosaminidase, chitobiosidase and endo- chitinase in solutions and on gels.. Analytical Biochemistry, 208,:74–79
    [Google Scholar]
  30. Tronsmo, A., Hjeljord, L., Klemsdal, S. S., V3rum, K. M., Nordtveit Hjerde, R. et al. (1996) Chitinolytic enzymes from the biocontrol agent Trichoderma harzianum.. Edited by Muzzarelli, R. A. A. Chitin Enzymology. vol. 2: Grottammare:: Atec Edizione,;235–244
    [Google Scholar]
  31. Trudel, J., Asselin, A. (1989); Detection of chitinase activity after polyacrylamide gel electrophoresis.. Analytical Biochemistry, 178,:362–366
    [Google Scholar]
  32. Ulhoa, C. J., Peberdy, J. F. (1991); Regulation of chitinase synthesis in Trichoderma harzianum.. Journal of General Microbiology, 137,:2163–2169
    [Google Scholar]
  33. Vogel, H. J. (1964); Distribution of lysine pathways among fungi: evolutionary implications.. American Naturalist, 98,:435–446
    [Google Scholar]
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