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Volume 144, Issue 6

Inducible chitinolytic system of Free

Abstract

Incubation of NCPF 2140 in growth medium containing 1 % chitin as sole carbon source led to induction of specific extracellular chitinolytic activity of 1.5 μmol GlcNAc released min (mg protein)-1. The effect was repressed by the inclusion of GlcNAc in the medium, indicating regulation by a negative feedback mechanism. Extracellular chitinase activity was inhibited by allosamidin (IC 0.12 μM). Multiple chitinolytic enzymes were detected on zymograms of extracellular preparations; levels of individual enzymes induced were dependent upon whether cells were incubated with purified colloidal chitin or a crude preparation of crystalline chitin. A major, inducible, 45 kDa chitinase was purified using ammonium sulphate precipitation, chitin affinity chromatography and a novel procedure involving the electroelution of the enzyme from a substrate gel containing glycol chitin. The enzyme is a glycoprotein with endochitinase activity.

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Keyword(s): Aspergillus fumigatus, , enzyme induction , extracellular chitinase and glycoprotein
© Society Society for General Microbiology, 1998
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1998-06-01
2024-04-25
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Inducible chitinolytic system of Aspergillus fumigatus
Microbiology 144, 1575 (1998); https://doi.org/10.1099/00221287-144-6-1575
/content/journal/micro/10.1099/00221287-144-6-1575
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