1887

Abstract

LPSs play an important role in bacterial pathogenesis. In this study, the LPS expression of the seven known species and its dependency on growth temperature was analysed by oxidative silver staining of proteinase-K-treated whole bacteria separated by Tricine-SDS-PAGE. The bordetellae were found to have extensively variable LPS in a species-specific way. In addition, the human and ovine strains exhibited host-specific LPS expression. LPSs from human strains grown at 37 and 25 °C were distinct. Growth temperature also affected LPS production by several strains. In some of these cases, BvgAS, the global regulator of virulence factors, was involved in this regulation of LPS biosynthesis. In contrast, no evidence was found for the involvement of the system in regulation of LPS synthesis. The obligate human pathogens and are closely related but were shown to produce immunologically distinct LPSs. These species are isolated from the upper respiratory tract and blood, respectively. This raises several interesting questions concerning the potential role of LPS as a virulence factor in the infection processes.

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1998-06-01
2021-05-09
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References

  1. Akerley, B. J., Miller, J. F. (1996); Understanding signal transduction during bacterial infection.. Trends in Microbiology, 4,:141–146
    [Google Scholar]
  2. van den Akker, W. M. R. (1997); Bordetella bronchiseptica has a BvgAS-controlled cytotoxic effect upon interaction with epithelial cells.. Fems Microbiology Letters, 156,:239–244
    [Google Scholar]
  3. Allen, A., Maskell, D. (1996); The identification, cloning and mutagenesis of a genetic locus required for lipopolysaccharide biosynthesis in Bordetella pertussis.. Molecular Microbiology, 19,:37–52
    [Google Scholar]
  4. Banemann, A., Gross, R. (1997); Phase variation affects longterm survival of Bordetella bronchiseptica in professional phagocytes.. Infection and Immunity, 65,:3469–3473
    [Google Scholar]
  5. Caroff, M., Chaby, R., Karibian, D., Perry, J., Deprun, C. et al. (1990); Variations in the carbohydrate regions of Bordetella pertussis lipopolysaccharides: electrophoretic, serological, and structural features.. ] Bacteriol, 172,:1121–1128
    [Google Scholar]
  6. Chaby, R., Caroff, M. (1988); Lipooligosaccharides of Bordetella pertussis endotoxin.. Edited by Wardlaw A. C., Parton. R. Pathogenesis and Immunity in Pertussis. Chichester:: Wiley,;247–271
    [Google Scholar]
  7. Cullinane, L. C., Alley, M. R., Marshall, R. B., Manktelow, B. W. (1987); Bordetella parapertussis from lambs.. N Z Vet J, 35,:175
    [Google Scholar]
  8. Di Fabio, J. L., Caroff, M., Karibian, D., Richards, J. C., Perry, M. B. (1992); Characterization of the common antigenic lipopolysaccharide O-chains produced by Bordetella bronchiseptica and Bordetella parapertussis.. Fems Microbiology Letters, 97,:275–282
    [Google Scholar]
  9. Ewanowich, C. A., Melton, A. R., Weiss, A. A., Sherburne, R. K., Peppier, M. S. (1989); Invasion of HeLa 229 cells by virulent Bordetella pertussis.. Infection and Immunity, 57,:2698–2704
    [Google Scholar]
  10. Gentry-Weeks, C. R., Provence, D. L., Keith, J. M., Curtiss, R. Ill et al. (1991); Isolation and characterization of Bordetella avium phase variants.. Infection and Immunity, 59,:4026–4033
    [Google Scholar]
  11. Goodnow, R. A. (1980); Biology of Bordetella bronchiseptica.. Microbiological Reviews, 44,:722–738
    [Google Scholar]
  12. Hitchcock, P. J., Brown T. M. (1983); Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels.. Journal of Bacteriology, 154,:269–277
    [Google Scholar]
  13. Holst, O., Ulmer, A. J., Brade, H., Flad, H.-D., Rietschel, E. T. (1996); Biochemistry and cell biology of bacterial endotoxins.. FEMS Immunol Med Microbiol, 16,:83–104
    [Google Scholar]
  14. Hertle, K.-H., Segers, A., Lievens, P., Siegmann, O., De Ley, J. (1984); Bordetella avium sp. nov., isolated from the respiratory tracts of turkeys and other birds.. International Journal of Systematic Bacteriology, 34,:56–70
    [Google Scholar]
  15. Laemmli, U. K. (1970); Cleavage of structural proteins during the assembly of the head of bacteriophage T4.. Nature, 227,:680–685
    [Google Scholar]
  16. Le Blay, K., Caroff, M., Richards, J. C., Perry, M. B., Chaby, R. (1994); Specific and cross-reactive monoclonal antibodies to Bordetella parapertussis and Bordetella broncbiseptica lipopoly- saccharides.. Microbiology, 140,:2459–2465
    [Google Scholar]
  17. Le Dur, A., Chaby, R., Szabó, L. (1980); Isolation of two protein- free and chemically different lipopolysaccharides from Bordetella pertussis phenol-extracted endotoxin.. / Bacteriol, 143,:78–88
    [Google Scholar]
  18. Lesse, A. J., Campagnari, A. A., Bittner, W. E., Apicella, M. A. (1990); Increased resolution of lipopolysaccharides and lipo- oligosaccharides utilizing tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis.. Journal of Immunological Methods, 126,:109–117
    [Google Scholar]
  19. Lindquist, S. W., Weber, D. J., Mangum, M. E., Hollis, D. G., Jordan, J. (1995); Bordetella holmesii sepsis in an asplenic adolescent.. Pediatric Infectious Disease Journal, 14,:813–815
    [Google Scholar]
  20. Linnemann, C. C., Perry E. B. (1977); Bordetella parapertussis. Recent experience and a review of the literature.. American Journal of Diseases of Children, 131,:560–563
    [Google Scholar]
  21. Mertsola, J. (1985); Mixed outbreak of Bordetella pertussis and Bordetella parapertussis infection in Finland.. European Journal of Clinical Microbiology & Infectious Diseases, 4,:123–128
    [Google Scholar]
  22. Mooi, F. R., van der Heide, H. G. J., ter Avest, A. R., Welinder, K. G., Livey, I. et al. (1987); Characterization of fimbrial subunits from Bordetella species.. Microb Pathog, 12,:127–135
    [Google Scholar]
  23. MUller, M., Hildebrandt, A. (1993) Nucleotide sequences of the 23S rRNA genes from Bordetella pertussis, avium, and their implications for phylogenetic analysis.. Edited by parapertussis, B., bronchiseptica, B. Nucleic Acids Res. 21,:3320
    [Google Scholar]
  24. Musser, J. M., Hewlett, E. L., Peppier, M. S., Selander, R. K. (1986); Genetic diversity and relationships in populations of Bordetella species.. Journal of Bacteriology, 166,:230–237
    [Google Scholar]
  25. Peppier, M. S. (1984); Two physically and serologically distinct lipopolysaccharide profiles of Bordetella pertussis and their phenotypic variants.. Infection and Immunity, 43,:224–232
    [Google Scholar]
  26. Peppier, M. S., Schrumpf, M. E. (1984); Phenotypic variation and modulation in Bordetella bronchiseptica.. Infection and Immunity, 44,:681–687
    [Google Scholar]
  27. Pittman, M. (1984) Genus Bordetella Moreno-Lopez 1952,178AL.. Edited by Krieg, N. R., Holt, J. G. Bergey's Manual of Systematic Bacteriology. vol. 1: Baltimore:: Williams &C Wilkins,;388–393
    [Google Scholar]
  28. Poolman, J. T., Kuipers, B., Vogel, M. L., Hamstra, H. J., Nagel, J. (1990); Description of a hybridoma bank towards Bordetella pertussis toxin and surface antigens.. Microb Pathog, 8,:377–382
    [Google Scholar]
  29. Porter, J. F., Connor, K., Donachie, W. (1994); Isolation and characterization of Bordetella parapertussis-like bacteria from ovine lungs.. Microbiology, 140,:255–261
    [Google Scholar]
  30. Porter, J. F., Connor, K., van der Zee, A., Reubsaet, F., Ibsen, P., Heron, I., Chaby, R., Le Blay, K., Donachie, W. (1995); Characterisation of ovine Bordetella parapertussis isolates by analysis of specific endotoxin (lipopolysaccharide) epitopes, filamentous haemagglutinin production, cellular fatty acid composition and antibiotic sensitivity.. Fems Microbiology Letters, 132,:195–201
    [Google Scholar]
  31. Preston, A., Mandrell, R. E., Gibson, B. W., Apicella, M. A. (1996); The lipooligosaccharides of pathogenic Gram-negative bacteria.. Crc Critical Reviews in Microbiology, 22,:139–180
    [Google Scholar]
  32. Reiman, D. A. (1995) Bordetella pertussis: determinants of virulence. In Bacterial Toxins and Virulence Factors in Disease.. Edited by Moss, J., Iglewski, B., Vaughan, M., Tu. A. T. Flandbook of Natural Toxins. vol. 8: New York:: Marcel Dekker,;367–405
    [Google Scholar]
  33. Stibitz, S., Miller, J. F. (1994) Coordinate regulation of virulence in Bordetella pertussis mediated by the vir {bvg) locus.. Edited by Miller, V. L., Kaper, J. B., Portnoy, D. A., Isberg. R. R. Molecular Genetics of Bacterial Pathogenesis. Washington, DC:: American Society for Microbiology,;407–422
    [Google Scholar]
  34. Tsai, C.-M., Frasch, C. E. (1982); A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels.. Anal Bio- chem, 119,:115–119
    [Google Scholar]
  35. Vandamme, P., Hommez, J., Vancanneyt, M., Monsieurs, M., Hoste, B. et al. (1995); Bordetella hinzii sp. nov., isolated from poultry and humans.. International Journal of Systematic Bacteriology, 45,:37–45
    [Google Scholar]
  36. Vandamme, P., Heyndrickx, M., Vancanneyt, M., Hoste, B., de Vos, P. et al. (1996); Bordetella trematum sp. nov., isolated from wounds and ear infections in humans, and reassessment of Alcaligenes denitrificans Riiger and Tan 1983.. International Journal of Systematic Bacteriology, 46,:849–858
    [Google Scholar]
  37. Verwey, W. F., Thiele, E. H., Sage, D. N., Schuchardt, L. F. (1949); A simplified liquid medium for the growth of Haemophilus pertussis.. Journal of Bacteriology, 58,:127–134
    [Google Scholar]
  38. Weiss, A. A., Falkow, S. (1984); Genetic analysis of phase change in Bordetella pertussis.. Infection and Immunity, 43,:263–269
    [Google Scholar]
  39. Weiss, A. A., Hewlett, E. L., Myers, G. A., Falkow, S. (1983); Tn-5 induced mutations affecting virulence factors of Bordetella pertussis.. Infection and Immunity, 42,:33–41
    [Google Scholar]
  40. Weyant, R. S., Hollis, D. G., Weaver, R. E. (1995) & 7 other authors, Bordetella holmesii sp. nov., a new gram-negative species associated with septicemia.. Journal of Clinical Microbiology, 33,:1–7
    [Google Scholar]
  41. Willems, R. J. L., van der Heide, H. G. J., Mooi, F. R. (1992); Characterization of a Bordetella pertussis fimbrial gene cluster which is located directly downstream of the filamentous haemagglutinin gene.. Molecular Microbiology, 6,:2661–2671
    [Google Scholar]
  42. Zarrouk, H., Karibian, D., Bodie, S., Perry, M. B., Richards, J. C. et al. (1997); Structural characterization of the lipid A of three Bordetella bronchiseptica strains: variability of fatty acid substitution.. Journal of Bacteriology, 179,:3756–3760
    [Google Scholar]
  43. van der Zee, A., Agterberg, C., van Agterveld, M., Peeters, M., Mooi, F. R. (1993); Characterization of IS1001, an insertion sequence element of Bordetella parapertussis.. Journal of Bacteriology, 175,:141–147
    [Google Scholar]
  44. van der Zee, A., Groenendijk, H., Peeters, M., Mooi, F. R. (1996); The differentiation of Bordetella parapertussis and Bordetella bronchiseptica from humans and animals as determined by DNA polymorphism mediated by two different insertion sequence elements suggests their phylogenetic relationship,. lnt J Syst Bacteriol, 46,:640–647
    [Google Scholar]
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