1887

Abstract

The zygomycete fungus (previously called ) secretes an aspartic proteinase containing two asparagine-linked, high-mannose type oligosaccharide chains at Asn and Asn. For structural elucidation of the carbohydrate moieties, the protein was divided into two portions, an N-terminal portion containing Asn and a C-terminal portion containing Asn, by a specific autocatalytic cleavage under alkaline conditions. Each of the asparagine-linked oligosaccharides was then released by peptide--glycosidase F digestion and pyridylaminated with a fluorescent reagent, 2-aminopyridine, at the reducing end. High-performance liquid chromatography analyses showed that the structure of the asparagine-linked oligosaccharide chain attached to residue Asn was Man GlcNAc, and that bound to residue Asn was Man GlcNAc and ManGlcNAc. These observations suggest that the processing of mannose residues in asparagine-linked oligosaccharides in the Golgi apparatus of resembles that in mammalian cells.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-144-5-1369
1998-05-01
2021-07-31
Loading full text...

Full text loading...

/deliver/fulltext/micro/144/5/mic-144-5-1369.html?itemId=/content/journal/micro/10.1099/00221287-144-5-1369&mimeType=html&fmt=ahah

References

  1. Aikawa, i, Yamashita, T., Nishiyama, M., Horinouchi, S., Beppu, T. (1990); Effect of glycosylation on the secretion and enzyme activity of Mucor rennin, an aspartic proteinase of Mucor pusillus, produced by recombinant yeast.. Journal of Biological Chemistry 265:(23)13955–13959 [View Article]
    [Google Scholar]
  2. Arima, K., Iwasaki, S., Tamura, G. (1967); Milk clotting enzyme from micro-organisms. Part I. Screening test and the identification of potent fungus.. Agric Biol Chem 31:540–545
    [Google Scholar]
  3. Arima, K., Yu, J., Iwasaki, S., Tamura, G. (1968); Milk-clotting enzyme from micro-organisms. V. Purification and crystallization of Mucor rennin from Mucor pusillus var.. Lindt. Appl Microbiol 16:(11)1727–1733 [View Article]
    [Google Scholar]
  4. Chu, F. K. (1986); Requirements of cleavage of high mannose oligosaccharides in glycoproteins by peptide N-glycosidase F.. Journal of Biological Chemistry 261:(1)172–177 [View Article]
    [Google Scholar]
  5. Etoh, Y., Shoun, H., Arima, K., Beppu, T. (1982); Photo-oxidation on a histidyl residue of milk-clotting acid protease, Mucor rennin.. Journal of Biochemistry 91:(3)747–753 [View Article]
    [Google Scholar]
  6. Hardy, N. R., Townsend, R. R., Lee, Y. C. (1988); Monosaccharide analysis of glycoconjugates by anion-exchange chromatography with pulsed amperometric detection.. Analytical Biochemistry 170:(1)54–62 [View Article]
    [Google Scholar]
  7. Hase, S., Kikuchi, N., Ikenaka, T., Inoue, K. (1985); Structures of sugar chains of the third component of human complement.. ] Biochem 98:863–874
    [Google Scholar]
  8. Hiramatsu, R., Horinouchi, S., Uchida, E., Hayakawa, T., Beppu, T. (1991); The secretion leader of Mucor pusillus rennin which possesses an artificial Lys-Arg sequence directs secretion of mature human growth hormone by Saccharomyces cerevisiae.. Appl Environ Microbiol 57:(7)2052–2056 [View Article]
    [Google Scholar]
  9. Iwasaki, S., Tamura, G., Arima, K. (1967); Milk clotting enzyme from micro-organisms. Part II. The enzyme production and the properties of crude enzyme.. Agric Biol Chem 31:540–545
    [Google Scholar]
  10. Kornfeld, R., Kornfeld, S. (1985); Assembly of asparagine-linked oligosaccharides.. Annual Review of Biochemistry 54:(1)631–664 [View Article]
    [Google Scholar]
  11. Kukuruzinska, M. A., Bergh, M. L. E., Jackson, B. J. (1987); Protein glycosylation in yeast.. Annual Review of Biochemistry 56:(1)915–944 [View Article]
    [Google Scholar]
  12. MacKinlay, A. G., Wake, R. G. (1971) K-Casein and its attack by rennin (chymosin).. Edited by McKenzie, H. A. Milk Proteins. vol. 2 New York:: Academic Press,;175–215
    [Google Scholar]
  13. Murakami, K., Aikawa, J., Horinouchi, S., Beppu, T. (1993); Characterization of an aspartic proteinase of Mucor pusillus expressed in Aspergillus oryzae.. Molecular & General Genetics 241:(3–4)312–318 [View Article]
    [Google Scholar]
  14. Murakami, K., Aikawa, J., Wada, M., Horinouchi, S., Beppu, T. (1994); A Mucor pusillus mutant defective in asparagine-linked glycosylation.. ] Bacteriol 176:(9)2635–2639 [View Article]
    [Google Scholar]
  15. Pitts, J. E., Dhanaraj, V., Dealwis, C. G., Mantafounis, D., Nugent, P. et al. (1992); Multidisciplinary cycles for protein engineering: site- directed mutagenesis and X-ray structural studies of aspartic proteinases.. Scand J Clin Lab Invest 52:(sup210)39–50 [View Article]
    [Google Scholar]
  16. Salovuori, I., Makarow, M., Rauvala, H., Knowles, J., Kaariainen, L. (1987); Low molecular weight high-mannose type glycans in a secreted protein of the filamentous fungus Trichoderma reesei.. Bio/Technology 5:152–156
    [Google Scholar]
  17. Takegawa, K., Kawasaki, N., Iwahara Y., Tochi-kura T., Mikami, B., Morita, Y. (1989); Primary structure of an N-linked sugar chain derived from glucoamylase of Rhizopus niveus.. Biochimica Et Biophysica Acta 990:(1)98–100 [View Article]
    [Google Scholar]
  18. Tarentino, A. L., Plummer, T. H. J., Maley F. (1974); The release of intact oligosaccharides from specific glycoproteins by endo-jS- N-acetylglucosaminidase H.. Journal of Biological Chemistry 249:(3)818–824 [View Article]
    [Google Scholar]
  19. Tomiya, N., Awaya, J., Kurono, M., Endo, S., Arata, Y. et al. (1988); Analyses of N-linked oligosaccharides using a two-dimensional mapping technique, Cloning and sequencing of a gene for Mucor rennin, an aspartic protease from Mucor pusillus.. Analytical Biochemistry 171:(1)73–90 [View Article]
    [Google Scholar]
  20. Yamashita, T., Tonouchi, N., Uozumi, T., Beppu, T. (1987); Secretion of Mucor rennin, a fungal aspartic protease of Mucor pusillus, by recombinant yeast cells.. Mol Gen Genet 210,462—467.
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-144-5-1369
Loading
/content/journal/micro/10.1099/00221287-144-5-1369
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error