1887

Abstract

When co-cultivated with chitin-containing fungi, secretes the chitin-binding protein CHB2. Microscopical and immunological investigations revealed that CHBZ acts like a glue to mediate the contact between the fungal and the Streptomyces hyphae. CHBZ was purified to homogeneity, and the sequence of its N-terminal amino acids was determined and used to deduce an oligonucleotide, which was then used to probe a subgenomic library. The gene was cloned, sequenced and overexpressed. The deduced mature protein has a molecular mass of 18.6 kDa, and a large number of its amino acids are identical to those of CHBl from . CHB2 effectively targets different types of α-chitin, but no other polysaccharide. The dissociation constant (K) for binding to purified crab shell chitin is 0 27 μM. Immunological studies suggest that homologues of CHBl and CHBZ are secreted by streptomycetes while growing in the presence of a-chitin-containing substrates.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-144-5-1291
1998-05-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/144/5/mic-144-5-1291.html?itemId=/content/journal/micro/10.1099/00221287-144-5-1291&mimeType=html&fmt=ahah

References

  1. Beyer, M., Diekmann, H. (1985); The chitinase system of Streptomyces sp. ATCC 11238 and its significance for fungal cell wall degradation.. Appl Microbiol Biotechnol 23:140–146
    [Google Scholar]
  2. Blaak, H., Schrempf, H. (1995); Binding and substrate specificities of a Streptomyces olivaceoviridis chitinase in comparison with its proteolytically processed form.. Eur J Biocbem 229:(1)132–139 [View Article]
    [Google Scholar]
  3. Blaak, H., Schnellmann, J., Walter, S., Henrissat, B., Schrempf, H. (1993); Characteristics of an exochitinase from Streptomyces olivaceoviridis, its corresponding gene, putative protein domains and relationship to other chitinases.. Eur ] Biocbem 214:(3)659–669 [View Article]
    [Google Scholar]
  4. Dittrich, W., Betzler, M., Schrempf, H. (1991); An amplifiable and deletable chloramphenicol-resistance determinant of Streptomyces lividans 1326 encodes a putative transmembrane protein.. Molecular Microbiology 5:(11)2789–2797 [View Article]
    [Google Scholar]
  5. Gilkes, N. R., Jervis, E., Henrissat, B., Tekant, B., Miller, R. C. Jr et al. (1992); The adsorption of a bacterial cellulase and its two isolated domains to crystalline cellulose.. J Biol Cbem 267:(10)6743–6749 [View Article]
    [Google Scholar]
  6. Goldstein, M. A., Takagi, M., Hashida, S., Shoseyov, O., R., H. et al. (1993); Characterization of the cellulose-binding domain of the Clostridium cellulovorans cellulose-binding protein A.. Journal of Bacteriology 175:(18)5762–5768 [View Article]
    [Google Scholar]
  7. Hopwood, D. A., Bibb, M. J., Chater, K. F. & 7 other authors, 1985, Genetic Manipulation of Streptomyces-. a Laboratory Manual Norwich:: John Innes Foundation;
    [Google Scholar]
  8. Jeuniaux, C. (1966); Chitinases.. Methods in Enzymology, Vol 46: Research on Nitrification and Related Processes, Pt B 8:644–650
    [Google Scholar]
  9. Laemmli, U. K. (1970); Cleavage of structural proteins during the assembly of the head of bacteriophage T4.. Nature 227:(5259)680–685 [View Article]
    [Google Scholar]
  10. Miyashita, K., Fujii, T., Watanabe, A., Ueno, H., Muzzarelli, R. A. A. et al. (1997) Nucleotide sequence and expression of a gene {chiB) for a chitinase from Streptomyces lividans, Chitin. J Ferment Bioeng . vol. 83, 2nd edn. edition, Oxford, Molecular Cloning:: Pergamon,;26–31 1977
    [Google Scholar]
  11. Schlochtermeier, A., Walter, S., Schrbder, J., Moormann, M., Schrempf, H. (1992); The gene encoding the cellulase (Avicelase) Cell from Streptomyces reticuli and analysis of protein domains.. Molecular Microbiology 6:(23)3611–3621 [View Article]
    [Google Scholar]
  12. Schnellmann, J., Zeltins, A., Blaak, H., Schrempf, H. (1994); The novel lectin-like protein CHB1 is encoded by a chitin-inducible Streptomyces olivaceoviridis gene and binds specifically to a- chitin of fungi and other organisms.. Molecular Microbiology 13:(5)807–819 [View Article]
    [Google Scholar]
  13. Vara, J., Lewandowska-Skarbek, M., Wang, Y.-G., Donadio, S., Hutchinson, C. R. (1989); Cloning of genes governing the deoxysugar portion of the erythromycin biosynthesis pathway in Saccharopolyspora erythraea {Streptomyces erytbreus).. Journal of Bacteriology 171:(11)5872–5881 [View Article]
    [Google Scholar]
  14. Watanabe, T., Kobori, K., Miyashita, K., Fujii, T., Sakai, H. et al. (1993); Identification of glutamic acid-204 and aspartic acid-200 in chitinase-Al of Bacillus circulans WL-12 as essential residues for chitinase activity.. Journal of Biological Chemistry 268:(25)18567–18572 [View Article]
    [Google Scholar]
  15. West, S., Schrdder, J., Kunz, W. (1990); A multiple-staining procedure for the detection of different DNA fragments on a single blot.. Anal Biocbem 190:(2)254–258 [View Article]
    [Google Scholar]
  16. Zeltins, A., Schrempf, H. (1995); Visualization of a-chitin with a specific chitin-binding protein (CHB1) from Streptomyces olivaceoviridis.. Anal Biocbem 231:(2)287–294 [View Article]
    [Google Scholar]
  17. Zeltins, A., Schrempf, H. (1997); Specific interaction of the Streptomyces chitin-binding protein CHB1 with a-chitin: the role of individual tryptophan residues.. Eur J Biocbem 246:(2)557–564 [View Article]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-144-5-1291
Loading
/content/journal/micro/10.1099/00221287-144-5-1291
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error