1887

Abstract

The telomers of several linear plasmids of (formerly ) were studied. The plasmids pHG201, pHG204 and pHG205 carry proteins bound to their ends, as shown by gel retardation experiments. A sequence hybridizing with the terminal sequence of pHG207, a recombinant linear plasmid consisting of the left part of pHG204 and the right part of pHG205, which was analysed in a previous study by the authors, could be detected in all linear plasmids of the wild-type strains MR11 and MR22. However, only pHG204 and pHG206 carry terminal inverted repeats (TIRs) like pHG207. Cloning and sequencing of the terminal fragment of pHG204 revealed a nearly perfect TIR of 1016 bp. In contrast, the termini of pHG201 and pHG205 share little homology. Sequence analysis of the two end fragments of pHG201 revealed a similarity of only 65% within the terminal 34/32 bp and a perfect TIR of only 3 bp. The results support the assumption that long TIRs are not absolutely necessary for replication and maintenance of linear plasmids.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-144-5-1271
1998-05-01
2021-10-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/144/5/mic-144-5-1271.html?itemId=/content/journal/micro/10.1099/00221287-144-5-1271&mimeType=html&fmt=ahah

References

  1. Aggag, M., Schlegel, H. G. (1973); Studies on a Gram-positive hydrogen bacterium, Nocardia opaca lb.. I. Description and physiological characterization. Arch Microbiol 88:299–318
    [Google Scholar]
  2. Altschul, S. F., Gish, W., Miller, W., Myers, E. W., Lipman, D. J. (1990); Basic local alignment search tool.. J 215:(3)403–410 [View Article]
    [Google Scholar]
  3. Ausubel, F. M., Brent, R., Kingston, R. E., More, D. D., Seidman, J. G. et al. (1987) Current Protocols in Molecular Biology New York:: Wiley;
    [Google Scholar]
  4. Chang, P.-C., Cohen, S. N. (1994); Bidirectional replication from an internal origin in a linear Streptomyces plasmid.. Science 265:(5174)952–954 [View Article]
    [Google Scholar]
  5. Chen, C. W. (1996); Complications and implications of linear bacterial chromosomes.. Trends in Genetics 12:(5)192–196 [View Article]
    [Google Scholar]
  6. Chen, C. W., Yu, T.-W., Lin, Y.-S., Kieser, H. M., Hopwood, D. A. (1993); The conjugative plasmid SLP2 of Streptomyces lividans is a 50 kbp linear molecule.. Molecular Microbiology 7:(6)925–932 [View Article]
    [Google Scholar]
  7. Court, D. A., Bertrand, H. (1991); A method for sequencing uncloned termini of linear plasmids.. Nucleic Acids Research 19:(7)1714–1714 [View Article]
    [Google Scholar]
  8. Crespi, M., Messens, E., Caplan, A. B., Van Montagu M, Desomer, J. (1992); Fasciation induction by the phytopathogen Rhodococcus fascians depends upon a linear plasmid encoding a cytokinin synthase gene.. Embo Journal 11:(3)795–804 [View Article]
    [Google Scholar]
  9. Dabrock, B., Riedel, J., Bertram, J., Gottschalk, G. (1992); Isopropylbenzene (cumene) - a new inducer substrate for the isolation of trichloroethene-degrading bacteria.. Archives of Microbiology 158:(1)9–13 [View Article]
    [Google Scholar]
  10. Dabrock, B., KeBeler, M., Averhoff, B., Gottschalk, G. (1994); Identification and characterization of a transmissible linear plasmid from Rhodococcus erythropolis BD2 specifying isopropylbenzene and trichloroethene (TCE) catabolism.. Appl Environ Microbiol 60:(3)853–860 [View Article]
    [Google Scholar]
  11. Devereux, J., Haeberli, P., Smithies O. (1984); A comprehensive set of sequence analysis programs for the VAX.. Nucleic Acids Research 12:(1Part1)387–395 [View Article]
    [Google Scholar]
  12. Escarmis, C., Salas, M. (1981); Nucleotide sequence at the termini of the DNA of Bacillus subtilis phage ^29.. Proc Natl Acad Sci USA 78:(3)1446–1450 [View Article]
    [Google Scholar]
  13. Graham, F. L., Rudy, J., Brinkley, P. (1989); Infectious circular DNA of human adenovirus type 5: regeneration of viral DNA termini from molecules lacking terminal sequences.. Embo Journal 8:(7)2077–2085 [View Article]
    [Google Scholar]
  14. Gravius, B., Glocker, D., Pigac, J., Pandza, K., Hranueli, D., Cullum, J. (1994); The 387 kbp linear plasmid pPZGIOl of Streptomyces rimosus and its interactions with the chromosome.. Microbiology 140:(9)2271–2277 [View Article]
    [Google Scholar]
  15. Grzeszik, C., LUbbers, M., Reh, M., Schlegel, H. G. (1997a); Genes encoding the NAD-reducing hydrogenase of Rhodococcus opacus MR11.. Microbiology 143:(4)1271–1286 [View Article]
    [Google Scholar]
  16. Grzeszik, C., Roß, K., Schneider K., Reh, M., Schlegel, H. G. (1997b); Location, catalytic activity, and subunit composition of NAD-reducing hydrogenases of some Alcaligenes strains and Rhodococcus opacus MR22.. Archives of Microbiology 167:(2–3)172–176 [View Article]
    [Google Scholar]
  17. Hirochika, H., Sakaguchi, K. (1982); Analysis of linear plasmids isolated from Streptomyces: association of protein with the ends of the plasmid DNA.. Plasmid 7:(1)59–65 [View Article]
    [Google Scholar]
  18. Hirochika, H., Nakamura, K., Sakaguchi, K. (1984); A linear DNA plasmid from Streptomyces rochei with terminal repetition of 614 base pairs.. Embo Journal 3:(4)761–766 [View Article]
    [Google Scholar]
  19. Kalkus, J., Reh, M., Schlegel H. G. (1990); Hydrogen autotrophy of Nocardia opaca strains is encoded by linear megaplasmids.. ] Gen Microbiol 136:(6)1145–1151 [View Article]
    [Google Scholar]
  20. Kalkus, J., Dttrrie, C., Fischer, D., Reh, M., Schlegel, H. G. (1993); The giant linear plasmid pHG207 from Rhodococcus sp. encoding hydrogen autotrophy: characterization of the plasmid and its termini.. Journal of General Microbiology 139:(9)2055–2065 [View Article]
    [Google Scholar]
  21. Khandjian, E. W. (1987); Optimized hybridization of DNA blotted and fixed to nitrocellulose and nylon membranes.. Bio/ Technology 5:165–167
    [Google Scholar]
  22. Kinashi, H., Shimaji-Murayama, M., Hanafusa, T. (1991); Nucleotide sequence analysis of the unusually long terminal inverted repeats of a giant linear plasmid.. SCP1 Plasmid 26:(2)123–130 [View Article]
    [Google Scholar]
  23. Klatte, S., Kroppenstedt, R. M., Rainey, F. A. (1994); Rhodococcus opacus sp. nov., an unusual nutritionally versatile Rhodococcus species.. Systematic and Applied Microbiology 17:(3)355–360 [View Article]
    [Google Scholar]
  24. Leblond, P., Fischer, G., Francou, F.-X., Berger, F., Guerineau, M., Decaris, B. (1996); The unstable region of Streptomyces ambofaciens includes 210 kbp terminal inverted repeats flanking the extremities of the linear chromosomal DNA.. Molecular Microbiology 19:(2)261–271 [View Article]
    [Google Scholar]
  25. Lezhava, A., Mizukami, T., Kajitano, T., Kameoka, D., Redenbbach, M., Shinkawa, M., Nimi, O., Kinashi, H. (1995); Physical map of the linear chromosome of Streptomyces griseus.. Journal of Bacteriology 177:(22)6492–6498 [View Article]
    [Google Scholar]
  26. Lin, Y.-S., Kieser, H. M., Hopwood, D. A., Chen, C. W. (1993); The chromosomal DNA of Streptomyces lividans 66 is linear.. Molecular Microbiology 10:(5)923–933 [View Article]
    [Google Scholar]
  27. McClelland, M., Jones, R., Patel, Y., Nelson, M. (1987); Restriction endonucleases for pulsed field mapping of bacterial genomes.. Nucleic Acids Research 15:(15)5985–6005 [View Article]
    [Google Scholar]
  28. Mendez, J., Blanco, L., Esteban, J. A., Bernad, A., Salas, M. (1992); Initiation of φ29 DNA replication occurs at the second 3' nucleotide of the linear template: a sliding-back mechanism for protein-primed DNA replication.. Proc Natl Acad Sci USA 89:(20)9579–9583 [View Article]
    [Google Scholar]
  29. OelmUller, U., KrUger, N., SteinbUchel, A., Friedrich, C. G. (1990); Isolation of prokaryotic RNA and detection of specific messenger- RNA with biotinylated probes.. J Microbiol Methods 11:(1)72–81 [View Article]
    [Google Scholar]
  30. Picardeau, M., Vincent, V. (1997); Characterization of large linear plasmids in mycobacteria.. Journal of Bacteriology 179:(8)2753–2756 [View Article]
    [Google Scholar]
  31. Rainey, F. A., Burghardt, J., Kroppenstedt, R. M., Klatte, S., Stackebrandt, E. (1995); Phylogenetic analysis of the genera Rhodococcus and Nocardia and evidence for the evolutionary origin of the genus Nocardia from within the radiation of Rhodococcus species.. Microbiology 141:(2)523–528 [View Article]
    [Google Scholar]
  32. Sakaguchi, K. (1990); Invertrons, a class of structurally and functionally related genetic elements that includes linear DNA plasmids, transposable elements, and genomes of Adeno-type viruses.. Microbiological Reviews 54:(1)66–74 [View Article]
    [Google Scholar]
  33. Salas, M., Freire, R., Soengas, M. S., Esteban, J. A., Mendez, J. et al. (1995); Protein-nucleic acid interactions in bacteriophage ^29 DNA replication.. Fems Microbiology Reviews 17:73–82
    [Google Scholar]
  34. Sanger, F., Nicklen, H., Coulson, A. R. (1977); DNA sequencing with chain-terminating inhibitors.. Proc Natl Acad Sci USA 77:(5)2611–2615 [View Article]
    [Google Scholar]
  35. Schneider, K., Cammack, R., Schlegel, H. G. (1984); Content and localization of FMN, Fe—S clusters and nickel in the NAD-linked hydrogenase of Nocardia opaca lb.. European Journal of Biochemistry 142:(1)75–84 [View Article]
    [Google Scholar]
  36. Sensfuss, C., Reh, M., Schlegel, H. G. (1986); No correlation exists between the conjugative transfer of the autotrophic character and that of plasmids in Nocardia opaca strains.. Journal of General Microbiology 132:997–1007
    [Google Scholar]
  37. Shiftman, D., Cohen, S. N. (1992); Reconstruction of a Streptomyces linear replicon from separately cloned fragments: existence of a cryptic origin of circular replication within the linear plasmid.. Proc Natl Acad Sci USA 89:(13)6129–6133 [View Article]
    [Google Scholar]
  38. Tabor, S., Richardson, C. C. (1989); Selective inactivation of the exonuclease activity of bacteriophage T7 DNA polymerase by in vitro mutagenesis.. Journal of Biological Chemistry 264:(11)6447–6458 [View Article]
    [Google Scholar]
  39. Vantomme, R., Elia, S., Swings, J., De Ley, J. (1982); Coryne- bacterium fascians (Tilford 1936) Dowson 1942, the causal agent of leafy gall on lily crops in Belgium.. Parasitica 38:183–192
    [Google Scholar]
  40. Vieira, J., Messing, J. (1987); Production of single-stranded plasmid DNA.. Methods in Enzymology, Vol 46: Research on Nitrification and Related Processes, Pt B 153:3–11
    [Google Scholar]
  41. Volff, J.-N., Viell, P., Altenbuchner, J. (1997); Artificial circularization of the chromosome with concomitant deletion of its terminal inverted repeats enhances genetic instability and genome rearrangement in Streptomyces lividans.. Molecular & General Genetics 253:(6)753–760 [View Article]
    [Google Scholar]
  42. Waterbury, P. G., Lane, M. J. (1987); Generation of lambda phage concatemers for use as pulsed field electrophoresis size markers.. Nucleic Acids Research 15:(9)3930–3930 [View Article]
    [Google Scholar]
  43. Wu, X., Roy, K. L. (1993); Complete nucleotide sequence of a linear plasmid from Streptomyces clavuligerus and characterization of its RNA transcript.. Journal of Bacteriology 175:(1)37–52 [View Article]
    [Google Scholar]
  44. Yoshikawa, H., Friedmann, T., Ito, J. (1981); Nucleotide sequences at the termini of ^29 DNA.. Proc Natl Acad Sci USA 78:(3)1336–1340 [View Article]
    [Google Scholar]
  45. Zaborosch, C., Schneider, K., Schlegel, H. G., Kratzin, H. (1989); Comparison of the NH2-terminal amino acid sequences of the four non-identical subunits of the NAD-linked hydrogenases from Nocardia opaca lb and Alcaligenes eutrophus H16.. European Journal of Biochemistry 181:(1)175–180 [View Article]
    [Google Scholar]
  46. Zakrzewska-Czerwinska, J., Schrempf, H. (1992); Characterization of an autonomously replicating region from the Streptomyces lividans chromosome.. Journal of Bacteriology 174:(8)2688–2693 [View Article]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-144-5-1271
Loading
/content/journal/micro/10.1099/00221287-144-5-1271
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error