1887

Abstract

Four extradiol dioxygenase genes which encode enzymes active against catechol and substituted catechols were cloned from two different and their nucleotide sequences were determined. A catechol 2,3-dioxygenase gene () was shown to be identical to the previously described gene from the isopropylbenzene operon of Amino acid sequences deduced from the three other genes () were shown to have various degrees of homology to different extradiol dioxygenases. The EdoA and EdoB dioxygenases were classified as belonging to the third family of type I oxygenases and represented two new subfamilies, whereas the EdoD dioxygenase was a type II enzyme. Analysis of six strains revealed a wide distribution of the above dioxygenase genes. I1 was shown to harbour all four of the analysed dioxygenase genes. Nucleotide sequences homologous to the gene were present in all of the strains, including NCIMB 13065, which did not utilize any of the aromatic compounds analysed. The latter finding points to the existence of a silent pathway(s) for degradation of aromatic compounds in this

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-144-4-955
1998-04-01
2021-10-16
Loading full text...

Full text loading...

/deliver/fulltext/micro/144/4/mic-144-4-0955.html?itemId=/content/journal/micro/10.1099/00221287-144-4-955&mimeType=html&fmt=ahah

References

  1. Asturias J. A., Eltis L. D., Prucha M., Timmis K. N. (1994); Analysis of three 2,3-dihydroxybiphenyl 1,2-dioxygenases found in Rhodococcus globerulus P6.. Journal of Biological Chemistry 269:(10),7807–7815 [View Article]
    [Google Scholar]
  2. Curragh H., Flynn O., Larkin M. J., Stafford T. M., Hamilton J. T. G. et al. (1994); Haloalkane degradation and assimilation by Rhodococcus rhodochrous NCIMB 13064.. Microbiology 140:(6),1433–1442 [View Article]
    [Google Scholar]
  3. Eltis L. D., Bolin J. T. (1996); Evolutionary relationship among extradiol dioxygenases.. Journal of Bacteriology 178:(20),5930–5937 [View Article]
    [Google Scholar]
  4. Finnerty W. R. (1992); The biology and genetics of the genus Rhodococcus.. Annual Review of Microbiology 46:(1),193–218 [View Article]
    [Google Scholar]
  5. Gibson D. T. (1971); Assay of enzymes of aromatic metabolism.. Methods Microbiol, 6:463–478
    [Google Scholar]
  6. Han S., Eltis L. D., Timmis K. N., Muchmore S. W., Bolin J. T. (1995); Crystal structure of biphenyl-cleaving extradiol dioxygenase from PCB-degrading pseudomonad.. Science, 270:(5238),976–980 [View Article]
    [Google Scholar]
  7. Happe B., Eltis L. D., Poth H., Hedderich R., Timmis K. N. (1993); Characterization of 2,2',3-trihydroxybiphenyl dioxygenase, an extradiol dioxygenase from the dibenzofuran- and dibenzo-p-dioxin-degrading bacterium Spbingomonas sp. strain RW1.. Journal of Bacteriology, 175:(22),7313–7320 [View Article]
    [Google Scholar]
  8. Harayama S., Rekik M. (1989); Bacterial aromatic ring-cleavage enzymes are classified in two different gene families.. Journal of Biological Chemistry, 264:(26),15328–15333 [View Article]
    [Google Scholar]
  9. Harayama S., Rekik M., Wasserfallen A., Bairoch A. (1987); Evolutionary relationships between catabolic pathways for aromatics: conservation of gene order and nucleotide sequences of catechol oxidation genes of pWWO and NAH7 plasmids. Molecular & General Genetics, 210241–247
    [Google Scholar]
  10. Harayama S., Kok M., Neidle E. L. (1992); Functional and evolutionary relationships among diverse dioxygenases.. Annual Review of Microbiology, 46:(1),565–601 [View Article]
    [Google Scholar]
  11. Hauschild J. E., Masai E., Sugiyama K., Hatta T., Kimbara K. et al. (1996); Identification of an alternative 2,3- dihydroxybiphenyl 1,2-dioxygenase in Rhodococcus sp. strain RHA1 and cloning of the gene.. Appl Environ Microbiol, 62:(8),2940–2946 [View Article]
    [Google Scholar]
  12. Hayaishi O. (1974) Molecular Mechanism of Oxygen Activation New York:: Academic Press;
    [Google Scholar]
  13. Higgins D. G., Sharp P. M. (1988); clustal: a package for performing multiple sequence alignments on a microcomputer.. Gene, 73:(1),237–244 [View Article]
    [Google Scholar]
  14. Hofer B., Eltis L. D., Dowling D. N., Timmis K. N. (1993); Genetic analysis of a Pseudomonas locus encoding a pathway for biphenyl/polychlorinated biphenyl degradation. Gene, 13047–55
    [Google Scholar]
  15. Kabisch M., Fortnagel P. (1990); Nucleotide sequence of metapyrocatechase I (catechol 2,3-oxygenase) gene mpcl from Alcaligenes eutrophus JMP222.. Nucleic Acids Research, 18:(11),3405–3406 [View Article]
    [Google Scholar]
  16. Keen N. T., Tamaki S., Kobayashi D., Trollinger D. (1988); Improved broad-host-range plasmids for DNA cloning in Gramnegative bacteria. Gene, 70191–197
    [Google Scholar]
  17. Kesseler M., Dabbs E. R., Averhoff B., Gottschalk G. (1996); Studies on the isopropylbenzene 2,3-dioxygenase and 3-iso- propylcatechol 2,3-dioxygenase genes encoded by the linear plasmid of Rhodococcus erythropolis BD2. Microbiology, 1423241–3251
    [Google Scholar]
  18. Kim E., Kim Y., Kim C.-K. (1996); Genetic structures of genes encoding 2,3-dihydroxybiphenyl 1,2 dioxygenase and 2-hydroxy- 6-oxo-6-phenylhexa-2,4-dienoic acid hydrolase from biphenyl- and 4-chlorobiphenyl-degrading Pseudomonas sp. strain DJ-12.. Appl Environ Microbiol, 62:(1),262–265 [View Article]
    [Google Scholar]
  19. Kuhm A. E., Stolz A., Ngai K.-L., Knackmuss H. J. (1991); Purification and characterization of a 1,2-dihydroxynaphthalene dioxygenase from a bacterium that degrades naphthalenesulfonic acids. Journal of Bacteriology, 1733795–3802
    [Google Scholar]
  20. Kulakova A. N., Stafford T. M., Larkin M. J., Kulakov L. A. (1995); Plasmid pRTLl controlling 1-chloroalkane degradation by Rhodococcus rhodochrous NCIMB13064.. Plasmid, 33:(3),208217–217 [View Article]
    [Google Scholar]
  21. Kulakova A. N., Reid K. A., Larkin M. J., Allen C. C. R., Kulakov L. A. (1996); Isolation of Rhodococcus rhodochrous NCIMB13064 derivatives with new biodegradative abilities.. Fems Microbiology Letters, 145:(2),227–231 [View Article]
    [Google Scholar]
  22. Kulakova A. N., Larkin M. J., Kulakov L. A. (1997); The plasmid-located haloalkane dehalogenase gene from Rhodococcus rhodochrous NCIMB 13064. Microbiology, 143109–115
    [Google Scholar]
  23. Larkin M. J., Day M. J. (1986); The metabolism of carbaryl by three bacterial isolates, Pseudomonas spp. (NCIB12042 and 12043) and Rhodococcus sp. (NCIB12038) from garden soil.. Journal of Applied Bacteriology, 60:(3),233–242 [View Article]
    [Google Scholar]
  24. Maeda M., Chung S.-Y., Song E., Kudo T. (1995); Multiple genes encoding 2,3-dihydroxybiphenyl 1,2-dioxygenase in the Gram-positive polychlorinated biphenyl degrading bacterium Rhodococcus erythropolis TA421, isolated from termite ecosystem; (1995 Appl Environ Microbiol, 61:549–555
    [Google Scholar]
  25. Masai E., Yamada A., Healey J. M., Hatta T., Kimbara K. et al. (1995); Characterization of biphenyl catabolic genes of Gram-positive polychlorinated biphenyl degrader Rhodococcus sp. strain RHA1.. Appl Environ Microbiol, 61:(6),2079–2085 [View Article]
    [Google Scholar]
  26. Miller J. H. (1972) Experiments in Molecular Genetics Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory;
    [Google Scholar]
  27. Noda Y., Nishikawa S., Shozuka K.-l., Kadokura H., Nakajima H. et al. (1990); Molecular cloning of protocatechuate 4,5- dioxygenase genes of Pseudomonas paucimobilis.. Journal of Bacteriology, 172:(5),2704–2709 [View Article]
    [Google Scholar]
  28. Patel T. R., Barnsley E. A. (1980); Naphthalene metabolism by pseudomonads: purification and properties of 1,2-dihydroxynaphthalene oxygenase.. Journal of Bacteriology, 143:(2),668–673 [View Article]
    [Google Scholar]
  29. Pearson W. R., Lipman D. J. (1988); Improved tools for biological sequence comparison.. Proc Natl Acad Sci USA, 85:(8),2444–2448 [View Article]
    [Google Scholar]
  30. Sambrook J., Fritsch E. F., Maniatis T. (1989) Molecular Cloning: a Laboratory Manual, 2nd edn.. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory;
    [Google Scholar]
  31. Schuppler M., Mertens F., Schbn G., Gobel U. B. (1995); Molecular characterization of nocardioform actinomycetes in activated sludge by 16S rRNA analysis. Microbiology, 141513–521
    [Google Scholar]
  32. Shine J., Dalgarno L. (1974); The S\\'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosomal binding sites.. Proc Natl Acad Sci USA, 71:(4),1342–1346 [View Article]
    [Google Scholar]
  33. Van de Peer Y., De Wachter R. (1994); treecon for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment.. Computer Applications in The Biosciences, 10:569–570
    [Google Scholar]
  34. Zuckerkandl E., Pauling L. (1965) Evolutionary divergence and convergence in proteins.. Edited by Bruson V., Vogel H. J. In Evolving Genes and Proteins New York:: Academic Press;97–166
    [Google Scholar]
  35. Zukowski M. M., Gaffiney D. F., Speck D., Kauffman M., Findeli A. et al. (1983); Chromogenic identification of genetic regulatory signals in Bacillus subtilis based on expression of a cloned Pseudomonas gene.. Proc Natl Acad Sci USA, 80:(4),1101–1105 [View Article]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-144-4-955
Loading
/content/journal/micro/10.1099/00221287-144-4-955
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error