RT Journal Article SR Electronic(1) A1 Gerike, Ursula A1 Hough, David W. A1 Russell, Nicholas J. A1 Dyall-Smith, Michael L. A1 Danson, Michael J.YR 1998 T1 Citrate synthase and 2-methylcitrate synthase: structural, functional and evolutionary relationships JF Microbiology, VO 144 IS 4 SP 929 OP 935 DO https://doi.org/10.1099/00221287-144-4-929 PB Microbiology Society, SN 1465-2080, AB Following the complete sequencing of the Escherichia coli genome, it has been shown that the proposed second citrate synthase of this organism, recently described by the authors, is in fact a 2-methylcitrate synthase that possesses citrate synthase activity as a minor component. Whereas the hexameric citrate synthase is constitutively produced, the 2-methylcitrate synthase is induced during growth on propionate, and the catabolism of propionate to succinate and pyruvate via 2-methylcitrate is proposed. The citrate synthases of the psychrotolerant eubacterium DS2-3R, and of the thermophilic archaea Thermoplasma acidophilum and Pyrococcus furiosus, are approximately 40% identical in sequence to the Escherichia coli 2-methylcitrate synthase andalso possess 2-methylcitrate synthase activity. The data are discussed with respect tothe structure, function and evolution of citrate synthase and 2-methylcitrate synthase., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-144-4-929