The 172 kb prkA-addAB region from 83° to 97° of the chromosome contains several dysfunctional genes, the marker, many genes encoding transporter proteins, and the ubiquitous hit gene Free

Abstract

A 171 812 bp nucleotide sequence between and(83° to 97°) on the genetic map of the168 chromosome was determined and analysed. An accurate physical/genetic map of this previously poorly described chromosomal region was constructed. One hundred and seventy open reading frames (ORFs) were identified on this DNA fragment. These include the previously described genes andin this region corresponds to the marker. Among the striking features of this region are: an abundance of genes encoding (putative) transporter proteins, several dysfunctional genes, the ubiquitous gene, and five multidrug-resistance-like genes. These analyses have also revealed the existence of numerous paralogues of ORFs in this region: about two-thirds of the putative genes seem to have at least one paralogue in the

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1998-04-01
2024-03-29
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References

  1. Anagnostopoulos C., Piggot P.J., Hoch J.A. 1993 The genetic map of Bacillus subtilis. Sonenshein, A. L., Hoch J. A., Losick R. Bacillus subtilis and Other Gram-positive Bacteria: Biochemistry, Physiology, and Molecular Genetics Washington, DC:: American Society for Microbiology;425—461
    [Google Scholar]
  2. Barnes W.M. (1994); PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates.. Proc Natl Acad Sci USA 91:2216–2220 [View Article]
    [Google Scholar]
  3. Beall B., Moran C.P. Jr (1994); Cloning and characterization of spoVR, a gene from Bacillus subtilis involved in spore cortex formation.. Journal of Bacteriology 176:2003–2012 [View Article]
    [Google Scholar]
  4. Beijer L., Nilsson R., Holmberg C., Rutberg L. (1993); The glpP and glpF genes of the glycerol regulon in Bacillus subtilis.. Journal of General Microbiology 139:349–359 [View Article]
    [Google Scholar]
  5. Biaudet V., Samson F., Anagnostopoulos C., Ehrlich S.D., Bessiferes P.h. (1996); Computerized genetic map of Bacillus subtilis.. Microbiology 142:2669–2729 [View Article]
    [Google Scholar]
  6. Bron S. 1990 Plasmids.. In: Cutting CRHarwood Sc S M. eds. Molecular Biological Methods for Bacillus Chichester:: Wiley;75–174
    [Google Scholar]
  7. Bron S., Venema G. (1972); Ultraviolet inactivation and excision repair in Bacillus subtilis. I. Construction and characterization of a eightfold auxotrophic strain and two ultraviolet-sensitive derivatives.. Mutation Research 15:1–10 [View Article]
    [Google Scholar]
  8. Bult C.J., White O., Olsen G.J. (1996); & 37 other authors .
  9. Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii Science 273:1058–1073
    [Google Scholar]
  10. Cheng S., Chang S-Y, Gravitt P., Respess R. (1994); Long PCR.. Nature 369:684–685 [View Article]
    [Google Scholar]
  11. Dear S., Staden R. (1991); A sequence assembly and editing program for efficient management of large projects.. Nucleic Acids Research 19:3907–3911 [View Article]
    [Google Scholar]
  12. Dedonder R.A., Lepesant J-A, Lepesant-Kejzlarov& J., Billault A., Steinmetz M., Kunst F. (1977); Construction of a kit of .
  13. reference strains for rapid genetic mapping in Bacillus subtilis 168 Appl Environ Microbiol 33:989–993
    [Google Scholar]
  14. Fischer C., Geourjon C., Bourson C., Deutscher J. (1996); Cloning and characterization of the Bacillus subtilis prkA gene encoding a novel serine protein kinase.. Gene 168:55–60 [View Article]
    [Google Scholar]
  15. Fleischmann R.D., Adams M.D., White O. (1995); & 37 other authors, Whole-genome random sequencing and assembly of Haemophilus influenzae Rd.. Science 269:496–512 [View Article]
    [Google Scholar]
  16. Fraser C.M., Gocayne JD„ White. , O (1995); & 26 other authors, The minimal gene complement of Mycoplasma genitalium.. Science 270:397–403 [View Article]
    [Google Scholar]
  17. Goffeau A., Barrel! B.G., Bussey H. (1996); & 13 other authors, Life with 6000 genes.. Science 274:546–567 [View Article]
    [Google Scholar]
  18. Gowrishankar J. (1989); Nucleotide sequence of the osmoregulatory proU operon of Escherichia coli.. Journal of Bacteriology 171:1923–1931 [View Article]
    [Google Scholar]
  19. Hansson M., Hederstedt L. (1992); Cloning and characterization of the Bacillus subtilis hemEHY gene cluster, which encodes protoheme IX biosynthetic enzymes.. Journal of Bacteriology 174:8081–8093 [View Article]
    [Google Scholar]
  20. Harford N., Lepesant-Kejzlarova J., Lepesant J-A, Hamers R., Dedonder R. 1976 Genetic circularity and mapping of the replication origin region of the Bacillus subtilis chromosome.. In: Schlesinger D. eds. Microbiology -1976 Washington, DC:: American Society for Microbiology;28–34
    [Google Scholar]
  21. Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B-G, Herrmann R. (1996); Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae.. Nucleic Acids Research 24:4420–4449 [View Article]
    [Google Scholar]
  22. Holmberg G., Beijer L., Rutberg B., Rutberg L. (1990); Glycerol catabolism in Bacillus subtilis: nucleotide sequence of the genes encoding glycerol kinase (glpK) and glycerol-3-phosphate dehydrogenase (glpD).. Journal of General Microbiology 136:2367–2375 [View Article]
    [Google Scholar]
  23. Hulett F.M., Kim E.E., Bookstein G., Kapp N.V., Edwards G.W., Wyckoff H.W. (1991); Bacillus subtilis alkaline phosphatase III and IV. Cloning, sequencing, and comparisons of deduced amino acid squence with Escherichia coli alkaline phosphatase three-dimensional structure.. J Biol Chent 266:1077–1084 [View Article]
    [Google Scholar]
  24. Ish-Horowicz D., Burke F.J. (1981); Rapid and efficient cosmid cloning.. Nucleic Acids Research 9:2989–2999 [View Article]
    [Google Scholar]
  25. Itaya M., Tanaka T. (1991); Complete physical map of the.
  26. Bacillus subtilis 168 chromosome constructed by a gene-directed mutagenesis method Journal of Molecular Biology 220:631–648
    [Google Scholar]
  27. Jin S., Sonenshein A.L. (1994a); Identification of two distinct Bacillus subtilis citrate synthase genes.. Journal of Bacteriology 176:4669–4679 [View Article]
    [Google Scholar]
  28. Jin S., Sonenshein A.L. (1994b); Transcriptional regulation of Bacillus subtilis citrate synthase genes.. Journal of Bacteriology 176:4680–4690 [View Article]
    [Google Scholar]
  29. Kaneko T., Sato S., Kotani H. (1996) 21 other authors II Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp, Sequence determination of the entire genome and assignment of potential protein-coding regions.. strain PCC6803 3109–136
    [Google Scholar]
  30. Kontinen V.P., Saris P., Sarvas M. (1991); A gene (prsA) of Bacillus subtilis involved in a novel late stage of protein export.. Molecular Microbiology 5:1273–1283 [View Article]
    [Google Scholar]
  31. Kunst F., Ogasawara N., Moszer I. (1997); & 148 other authors.
  32. The complete genome sequence of the Gram-positive bacterium Bacillus subtilis Nature 390:249–256
    [Google Scholar]
  33. Lomovskaya O., Lewis K. (1992); Emr, an Escherichia coli locus for multidrug resistance.. Proc Natl Acad Sci USA 89:8938–8942 [View Article]
    [Google Scholar]
  34. Mandel M., Higa A. (1970); Calcium-dependent bacteriophage DNA infection.. Journal of Molecular Biology 53:159–162 [View Article]
    [Google Scholar]
  35. Mewes H.W., Albermann K., Bahr M. (1997); & 9 other authors .
  36. Overview of the yeast genome Nature 387:7–9
    [Google Scholar]
  37. Moszer I., Glaser P., Danchin A. (1995); SubtiList: a relational database for the Bacillus subtilis genome.. Microbiology 141:261–268 [View Article]
    [Google Scholar]
  38. Noback M.A., Terpstra P., Holsappel S., Venema G., Bron S. (1996); A 22 kb DNA sequence in the cspB—glpPFKD region at 75 ° on the Bacillus subtilis chromosome.. Microbiology 142:3021–3026 [View Article]
    [Google Scholar]
  39. O\\'Brien C. (1997); Entire E. coli genome sequenced - at last.. Nature 385:472–472 [View Article]
    [Google Scholar]
  40. Pearson J.D., DeWald D.B., Mathews W.R. (1990); & 10 other authors, Amino acid sequence and characterization of a protein inhibitor of protein kinase C.. } Biol Chem 265:4583–4591 [View Article]
    [Google Scholar]
  41. Pearson W.R., Lipman D.J. (1988); Improved tools for biological sequence comparison.. Proc Natl Acad Sci USA 85:2444–2448 [View Article]
    [Google Scholar]
  42. Perego M, Hoch J. A. (1988); Sequence analysis and regulation of the hpr locus, a regulatory gene for protease production and sporulation in Bacillus subtilis.. Journal of Bacteriology 170:2560–2567 [View Article]
    [Google Scholar]
  43. Sambrook J., Fritsch E.F., Maniatis T. (1989) Molecular Cloning: a Laboratory Manual Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory;
    [Google Scholar]
  44. Sanger F., Nicklen S., Coulsen A.R. (1977); DNA sequencing with chain-terminating inhibitors.. Proc Natl Acad Sci USA 74:5463–5467 [View Article]
    [Google Scholar]
  45. Seraphin B. (1992); The HIT protein family: a new family of proteins present in prokaryotes, yeast and mammals.. DNA Seq 3:177–179 [View Article]
    [Google Scholar]
  46. Stahl M.L., Ferrari E. (1984); Replacement of the Bacillus subtilis subtilisin structural gene with an in vitro-derived deletion mutation.. Journal of Bacteriology 158:411–418 [View Article]
    [Google Scholar]
  47. Stauffer G.V. 1983 Regulation of serine, glycine, and one- carbon biosynthesis.. In: Somerville KMHerrman Sc R L. eds. Amino Acids: Biosynthesis and Genetic Regulation Reading, MA:: Addison-Wesley;103–113
    [Google Scholar]
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