The 172 kb prkA-addAB region from 83° to 97° of the Bacillus subtilis chromosome contains several dysfunctional genes, the glyB marker, many genes encoding transporter proteins, and the ubiquitous hit gene
A 171 812 bp nucleotide sequence betweenprkA andaddAB(83° to 97°) on the genetic map of theBacillus subtilis168 chromosome was determined and analysed. An accurate physical/genetic map of this previously poorly described chromosomal region was constructed. One hundred and seventy open reading frames (ORFs) were identified on this DNA fragment. These include the previously described genes cspB, glpPFKD, spoVR, phoAIV, papQ, citRA, sspB, prsA, hpr, pbpF, hemEHY, aprE, comKandaddAB. ORF yhaFin this region corresponds to the glyB marker. Among the striking features of this region are: an abundance of genes encoding (putative) transporter proteins, several dysfunctional genes, the ubiquitous hit gene, and five multidrug-resistance-like genes. These analyses have also revealed the existence of numerous paralogues of ORFs in this region: about two-thirds of the putative genes seem to have at least one paralogue in theB. subtilis genome
AnagnostopoulosC.,
PiggotP.J.,
HochJ.A.1993 The genetic map of Bacillus subtilis. Sonenshein,A. L.,
HochJ. A.,
LosickR.Bacillus subtilis and Other Gram-positive Bacteria: Biochemistry, Physiology, and Molecular Genetics Washington, DC:: American Society for Microbiology;425—461
BarnesW.M.(1994); PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates.. Proc Natl Acad Sci USA 91:2216–2220 [View Article]
BeallB.,
MoranC.P.Jr(1994); Cloning and characterization of spoVR, a gene from Bacillus subtilis involved in spore cortex formation.. Journal of Bacteriology 176:2003–2012 [View Article]
BeijerL.,
NilssonR.,
HolmbergC.,
RutbergL.(1993); The glpP and glpF genes of the glycerol regulon in Bacillus subtilis.. Journal of General Microbiology 139:349–359 [View Article]
BronS.,
VenemaG.(1972); Ultraviolet inactivation and excision repair in Bacillus subtilis. I. Construction and characterization of a eightfold auxotrophic strain and two ultraviolet-sensitive derivatives.. Mutation Research 15:1–10 [View Article]
DearS.,
StadenR.(1991); A sequence assembly and editing program for efficient management of large projects.. Nucleic Acids Research 19:3907–3911 [View Article]
FischerC.,
GeourjonC.,
BoursonC.,
DeutscherJ.(1996); Cloning and characterization of the Bacillus subtilis prkA gene encoding a novel serine protein kinase.. Gene 168:55–60 [View Article]
FleischmannR.D.,
AdamsM.D.,
WhiteO.(1995); & 37 other authors, Whole-genome random sequencing and assembly of Haemophilus influenzae Rd.. Science 269:496–512 [View Article]
HanssonM.,
HederstedtL.(1992); Cloning and characterization of the Bacillus subtilis hemEHY gene cluster, which encodes protoheme IX biosynthetic enzymes.. Journal of Bacteriology 174:8081–8093 [View Article]
HarfordN.,
Lepesant-KejzlarovaJ.,
LepesantJ-A,
HamersR.,
DedonderR.1976 Genetic circularity and mapping of the replication origin region of the Bacillus subtilis chromosome.. In:
SchlesingerD.
eds. Microbiology -1976 Washington, DC:: American Society for Microbiology;28–34
HimmelreichR.,
HilbertH.,
PlagensH.,
PirklE.,
LiB-G,
HerrmannR.(1996); Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae.. Nucleic Acids Research 24:4420–4449 [View Article]
HolmbergG.,
BeijerL.,
RutbergB.,
RutbergL.(1990); Glycerol catabolism in Bacillus subtilis: nucleotide sequence of the genes encoding glycerol kinase (glpK) and glycerol-3-phosphate dehydrogenase (glpD).. Journal of General Microbiology 136:2367–2375 [View Article]
KanekoT.,
SatoS.,
KotaniH.(1996) 21 other authors II Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp, Sequence determination of the entire genome and assignment of potential protein-coding regions.. strain PCC68033109–136
KontinenV.P.,
SarisP.,
SarvasM.(1991); A gene (prsA) of Bacillus subtilis involved in a novel late stage of protein export.. Molecular Microbiology 5:1273–1283 [View Article]
NobackM.A.,
TerpstraP.,
HolsappelS.,
VenemaG.,
BronS.(1996); A 22 kb DNA sequence in the cspB—glpPFKD region at 75 ° on the Bacillus subtilis chromosome.. Microbiology 142:3021–3026 [View Article]
PearsonJ.D.,
DeWaldD.B.,
MathewsW.R.(1990); & 10 other authors, Amino acid sequence and characterization of a protein inhibitor of protein kinase C.. } Biol Chem 265:4583–4591 [View Article]
PeregoM,
HochJ. A.
(1988); Sequence analysis and regulation of the hpr locus, a regulatory gene for protease production and sporulation in Bacillus subtilis.. Journal of Bacteriology 170:2560–2567 [View Article]
StahlM.L.,
FerrariE.(1984); Replacement of the Bacillus subtilis subtilisin structural gene with an in vitro-derived deletion mutation.. Journal of Bacteriology 158:411–418 [View Article]
StaufferG.V.1983 Regulation of serine, glycine, and one- carbon biosynthesis.. In:
SomervilleKMHerrman Sc R L.
eds. Amino Acids: Biosynthesis and Genetic Regulation Reading, MA:: Addison-Wesley;103–113
The 172 kb prkA-addAB region from 83° to 97° of the Bacillus subtilis chromosome contains several dysfunctional genes, the glyB marker, many genes encoding transporter proteins, and the ubiquitous hit gene