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Volume 144, Issue 4

Fructose and mannose metabolism in Aeromonas hydrophila: identification transport systems and catabolic pathways Free

Abstract

was examined for fructose and mannose transport systems. was shown to possess a phosphoenolpyruvate (PEP): fructose phosphotransferase system (fructose-PTS) and a mannose-specific PTS, both induced by fructose and mannose. The mannose-PTS of exhibited cross-reactivity with mannose-PTS proteins. The fructose-PTS proteins exhibited cross-reactivities with and fructose-PTS proteins. In grown on mannose as well as on fructose, the phosphorylated derivative accumulated from fructose was fructose 1-phosphate. Identification of fructose 1-phosphate was confirmed by C-NMR spectroscopy. 1-Phosphofructokinase (1-PFK), which converts the product of the PTS reaction to fructose 1,6-diphosphate, was present in grown with fructose but not on mannose. An inducible phosphofructomutase (PFM) activity, an unusual enzyme converting fructose 1-phosphate to fructose 6-phosphate, was detected in extracts induced by mannose or fructose. These results suggest that in cells grown on fructose, fructose 1-phosphate could be converted to fructose 1,6-diphosphate either directly by the 1-PFK activity or via fructose 6-phosphate by the PFM and 6-phosphofructokinase activities. In cells grown on mannose, the degradation of fructose 1-phosphate via PFM and the Embden-Meyerhof pathway appeared to be a unique route.

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  • Accepted:
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Keyword(s): Aeromonas hydrophila , catabolic pathways , fructose , mannose and phosphotransferase system
© Society for General Microbiology 1998
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1998-04-01
2024-04-18
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Fructose and mannose metabolism in Aeromonas hydrophila: identification transport systems and catabolic pathways
Microbiology 144, 1113 (1998); https://doi.org/10.1099/00221287-144-4-1113
/content/journal/micro/10.1099/00221287-144-4-1113
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