RT Journal Article SR Electronic(1) A1 Gu, X. X. A1 Rossau, R. A1 Jannes, G. A1 Ballard, R. A1 Laga, M. A1 Van Dyck, EYR 1998 T1 The rrs (16S)-rrl (23S) ribosomal intergenic spacer region as a target for the detection of Haemophilus ducreyi by a heminested-PCR assay JF Microbiology, VO 144 IS 4 SP 1013 OP 1019 DO https://doi.org/10.1099/00221287-144-4-1013 PB Microbiology Society, SN 1465-2080, AB The intergenic spacer region between the rrs and rrl ribosomal RNA genes of Haemophilus ducreyi was analysed and the DNA sequence was used for the selection of specific PCR primers. A highly sensitive and specific heminested-PCR assay for the identification of H. ducreyi was developed. The assay showed a sensitivity of 96% on genital ulcer specimens from patients with clinically diagnosed chancroid, compared with a sensitivity of 56% for culture methods. These results indicate that this PCR assay has the potential to become an accurate and easy reference method for the detection of H. ducreyi., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-144-4-1013